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모유와 우유의 지질에 관한 연구 : I. 중성지질 함량 및 지방산 조성 I. Fatty Acid Compositions and Contents in Neutral Lipid
차광종,황주환,유제현 한국낙농학회 1985 韓國酪農學會誌 Vol.7 No.1
本 實驗은 母乳와 牛乳의 脂質構成을 Thin-layer chromatography(TLC)와 densitometry에 依해 比較, 檢討하고, Gas-liquid chromatography(GLC)에 依해 中性脂質 脂肪酸組成을 分析, 比較하여 調製粉乳 製造의 基礎的인 資料를 探索하기 爲해 實施하였으며, 그 結果를 要約하면 다음과 같다. 1. 總脂質은 母乳 3.36%, 牛乳 3.49%가 抽出되었으며, 그중에서 中性脂質의 比率은 各各 99.32%, 99.38였다. 2. 中性脂質構咸은 triglyceride가 母乳 92.76%, 牛乳93.15%로 가장 많았고, 1.3-diglyceried, free fatty acid, 1.2-diglyceride 等이 共通的으로 檢出되었으며, 特히 母乳는 牛乳보다 遊離脂肪酸含量이 높았다. 3. 中性脂質의 脂肪酸組成은 母乳는 牛乳와 比較하여 底賠防酸含量이 낮았지만 oleic acid와 linoleic acid 等 高級脂肪酸의 含量이 높았다. This experiment was carried out to compare and examine the composition of lipid in Human and Cow milk by Thin layer chromatography and Densitometry, to analyze and compare the composition of fatty acids of Neutral lipid by Gas-liquid chromatography and to get basic data for the manufacture of modified milk powder. The results obtained were as follows: 1. The total lipid content was 3.36% in human milk, 3.49% in cow milk, of which the percentage of neutral lipid was 99.32% and 99.38%, respectively. 2. The neutral lipid mainly consisted of triglyceride (92.76% in human milk, 93.15% in cow milk) and both milk samples contained 1.3-diglyceride, free fatty acid and 1.2-diglyceride. Especially, cow milk was much higher than human milk in the contents of free fatty acid. 3. As for the fatty acids composition of neutral lipid, the content of low molecular fatty acid was low in human milk in comparison with cow milk, But high molecular fatty acid such as oleic acid and linoleic acid were high in human milk.
리더쉽理論의 變遷過程에 관한 硏究 : Layiug Stress on Change of Process
車光鍾 단국대학교 대학원 1982 學術論叢 Vol.6 No.-
Someone says that modern society is the rapidly changing period. F. Emery and Trist said that the repidly changing period was the turbulent field. Our surrounding society is not the exception, too. We can call that all relations, including Korea, is the turbulent field. And we can not anticipate the complicated situations and the rapid changing of all relations, and can not control the circumstances. In these circumstances, we can recognize the rise of problems, how we can smoothly accomplish the national administration, and how we can formulate and perform actively all problems, for example, the protection of national rights and interests, the promotion of welfare. First of all, I am premised as the following, and I will examine the problems, that is, in all the developing country that is place in he turbulence of rapid social change, one of most important variables for national development is the leadership that the policy-making body can perfom actively the function as a reformer of social change and a administrator. Because only the policy-making body that has the readership can continuously assimilate the change of circumstances and he can lead the circumstances to the definite direction. In his premise, we will be confronted with the problems what is the mean of leadership, and how can we apply and develop the leadership. In this thesis, I have prescribed the notion of leadership, comparing with the opinions of numerous scholars and similar notions. And I have examined how such this theory on the leadership has changed.
차광종,송진욱,조홍찬,김용휘,유대열,이중복,이종기,곡구효보,유제현,Cha, Kwang-Jong,Song, Jin-Ook,Cho, Hong-Chan,Kim, Yong-Hee,Yu, Dae-Yeul,Lee, Joong-Bok,Lee, Chong-Kee,Koki, Taniguchi,Yu, Jae-Hyeun 대한미생물학회 1999 Journal of Bacteriology and Virology Vol.28 No.1
Rotaviruses belong to Reoviridae causes diarrhea in human beings as well as domestic animals. This study was conducted to see what type of human rotaviruses are distributed in Seoul and Kyung-gi province. Twenty two of 81 patients showed rotavirus positive with diagnostic kit and RNA electropherosis. We isolated all of rotaviruses from the patients. Electropherotypes of 22 isolates showed 4:2:3:2 pattern whereas those migration patterns were long type. All of those isolates belonged to group A. Twenty out of 22 isolates reacted with monoclonal antibodies specific to G1, P1A and subgroup II, whereas rest of them, A-29 and K-30 reacted with subgroup I specific monoclonal antibody. The nucleotide sequence of an isolate K-21 showed $98{\sim}100%$ and $90{\sim}96%$ homologies with those of Wa and KU strain, respectively.
락토페린이 국내분리 유아 로타바이러스의 MA 104세포 감염에 미치는 영향
차광종,유대열,이종기,유제현,Cha, Kwang-Jong,Yu, Dae-Yeul,Lee, Chong-Kee,Yu, Jae-Hyeun 대한미생물학회 1999 Journal of Bacteriology and Virology Vol.28 No.1
It has long been known that lactoferrin prevents human beings from infection of virus. To prove this activity of lactoferrin, we evaluated the activities of different lactoferrins to an isolate human rotavirus K-21. Bovine lactoferrin inhibited infection of K-21 to MA-104 cell at the concentration of $25.9\;{\mu}M$ whereas bovine hydrolysed lactoferrin prevented rotavirus infection at $103.8\;{\mu}M$. However human lactoferrin prevented infection of K-21 at the concentration of $217.5\;{\mu}M$. These data suggested that lactoferrin activity may be unaffected by the intestinal digestive enzymes and bovine lactoferrin is more active than human lactoferrin with respect to prevention of rotavirus infection.
락토페린이 국내분리 유아 로타바이러스의 MA 104세포 감염에 미치는 영향
차광종(Gwang Jong Cha),유대열(Dae Yeol Yoo),이종기(Jong Gee Lee),유제현(Je Hyun Yoo) 대한바이러스학회 1999 Journal of Bacteriology and Virology Vol.29 No.2
It has long been known that lactoferrin prevents human beings from infection of virus. To prove this activity of lactoferrin, we evaluated the activities of different lactoferrins to an isolate human rotavirus K-21. Bovine lactoferrin inhibited infection of K-21 to MA-104 cell at the concentration of 25.9 ㎛ whereas bovine hydrolysed lactoferrin prevented rotavirus infection at 103.8 ㎛. However human lactoferrin prevented infection of K-21 at the concentration of 217.5 ㎛. These data suggested that lactoferrin activity may be unaffected by the intestinal digestive enzymes and bovine lactoferrin is more active than human lactoferrin with respect to prevention of rotavirus infection.
차광종(Gwang Jong Cha),송진욱(Jin Wook Song),조홍찬(Hong Chan Jo),김용휘(Yong Hwi Kim),유대열(Dae Yeol Yoo),이중복(Joong Bok Lee),이종기(Jong Gee Lee),유제현(Je Hyun Yoo),Taniguchi Koki 대한바이러스학회 1999 Journal of Bacteriology and Virology Vol.29 No.2
Rotaviruses belong to Reoviridae causes diarrhea in human beings as well as domestic animals. This study was conducted to see what type of human rotaviruses are distributed in Seoul and Kyung-gi province. Twenty two of 81 patients showed rotavirus positive with diagnostic kit and RNA electropherosis. We isolated all of rotaviruses from the patients. Electropherotypes of 22 isolates showed 4:2:3:2 pattern whereas those migration patterns were long type. All of those isolates belonged to group A. Twenty out of 22 isolates reacted with monoclonal antibodies specific to G1, P1A and subgroup II, whereas rest of them, A-29 and K-30 reacted with subgroup I specific monoclonal antibody. The nucleotide sequence of an isolate K-21 showed 98-100% and 90-96% homologies with those of Wa and KU strain, respectively.
Mozzarella Cheese의 무기질 함량에 관한 연구
차광종 ( Gwang Jong Cha ),연제한 ( Je Han Yeon ),유제현 ( Je Hyeon Yu ) 한국유가공기술과학회 1996 Journal of Dairy Science and Biotechnology (JMSB) Vol.14 No.2
본 실험은 국내에서 생산, 판매되고 있는 5개사의 Mozzarella cheese 25개 제품을 구입 하여 일반 성분과 Ca, P, Mg, Na, K, Fe 등의 무기물을 분석, 비교하므로서 국산Mozzarella cheese의 품질을 검토하기 위하여 실시하였으며 그 결과는 다음과 같다. Mozzarella cheese의 평균 일반 조성은 수분 49.01%, 지방 22.54%, FiDM 44.16%, 단백질 24.36%, 회분 2.55%였으며 PH는 5.29였다. Mozzarella cheese의 평균 무기물 함량은 Ca 696.44mg, P 466.31mg, Mg 22.08mg, Na 310.8mg, K 82.84mg, Fe 0.36mg이었다. Ca, P, Mg 등은 C사 제품이 797.41mg, 536.61mg, 25.54mg으로 가장 높았고, Na은 A사 제품이 450.83mg으로 가장 높았으며, K, Fe 등은 B사제품이 98.92mg, 0.51mg으로 가장 높게 나타났다. 그리고 Ca, P의 비율은 1.5: 1로 적절한 수준을 유지했고, Mozzarella cheese 645g에는 Ca, P, Na 등의 무기물 1일 권장량이 충분히 함유된 것으로 나타났다. This experiment was carried out to find general composition and mineral contents such as Ca, P, Mg, Na, K and Fe of domestic commercials Mozzarella cheese. Twenty-five products of 5 company were analyzed. The results obtaied were as fellow: The average contents of moisture, fat, fat in dry matter, protein and ash in Mozzarella cheese were 49.01, 22.54, 44.16, 24.36 and 2.55%, respectively and average pH was 5.29. The average contents of Ca, P, Mg, Na, K and Fe were 696.44, 466.31, 22.08, 310.8, 82.84 and 0.36mg, respectively. Among products, product C was highest in levels of Ca, P and Mg, which were 797.4, 536.6 and 25.5mg, respectively, Na was highest for product A which contained 450.8mg. K and Fe were highest for product B which contained 98.9 and 0.51mg, respectively. Generally, the ratio of Ca to P was 1.5 : 1 and within the proper range. It was found that 645g of Mozzarella cheese was able to fully meet the daily recommendation of minerals including Ca, P and Na.