Pitch Measurement of 150 nm ID-grating Standards Using an Nanometrological Atomic Force Microscope

진종한,Ichiko Misumi,Satoshi Gonda,Tomizo Kurosawa 한국정밀공학회 2004 International Journal of Precision Engineering and Vol.5 No.3

Pitch measurements of 150 nm one-dimensional grating standards were carried out using a contact mode atomic force microscopy with a high resolution three-axis laser interferometer. This measurement technique was named as the nano-metrological AFM. In the nano-metrological AFM, three laser interferometers were aligned precisely to the end of an AFM tip. Laser sources of the three-axis laser interferometer in the nano-metrological AFM were calibrated with an I2-stabilized He-Ne laser at a wavelength of 633 nm. Therefore, the Abbe error was minimized and the result of the pitch measurement using the nano-metrological AFM could be used to directly measure the length standard. The uncertainty in the pitch measurement was estimated in accordance with the Guide to the Expression of Uncertainty in Measurement (GUM). The primary source of uncertainty in the pitch-measurements was derived from the repeatability of the pitch-measurements, and its value was about 0.186 nm. The average pitch value was 146.65 nm and the combined standard uncertainty was less than 0.262 nm. It is suggested that the metrological AFM is a useful tool for the nano-metrological standard calibration.

펨토초 펄스 레이저를 이용한 반도체/디스플레이 공정에서의 정밀 형상 측정 기술

진종한(Jonghan Jin),박정재(Jungjae Park),안흘비(Heulbi Ahn),배재석(Jaeseok Bae) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11

높은 동적 범위를 갖는 간섭 기반 두께 측정 기술

진종한(J. Jin),박정재(J. Park),배재석(J. Bae) Korean Society for Precision Engineering 2023 한국정밀공학회 학술발표대회 논문집 Vol.2023 No.11

OPU 유래 한우 수정란 생산 및 이식

진종인,최병현,김성수,조현태,선두원,임현태,이정규,민찬식,공일근 한국수정란이식학회 2014 한국동물생명공학회지 Vol.29 No.3

This study was carried out to establish the system of OPU derived embryo production, management of recipients as well as offspring production. OPU derived embryo production system was carried out of aspiration of immature oocytes 2 times per week, total 24 times for 3 months by an ultrasonographic guided follicular aspiration system and then produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture system. This work was collected total 13,866 oocytes, average 8.2±4.5 oocytes per session and 8,170 G1 + G2 grade oocytes, average 4.8 oocytes per session by 1,692 times session of total 71 donors for 4 years from 2010 to 2013. The rate of cleavage and blastocyst developmental competence were obtained 11,825 (85.3%) and 5,032 (36.3%) that was 7.0±3.8 cleaved embryos and 3.0±2.5 blastocysts per session. OPU derived embryo transfer were taken place in 2, 4, 6 and 7 local governments at 2010, 2011, 2012 and 2013 for 4 years and pregnancy rate were obtained 41.2, 43.9, 46.5 and 49.7% in each years. It means that pregnancy rate was continuously improved according of every year for 4 years. Pregnancy rate was significantly different according to individual local government in which was 62.7% in B, but 24.2% in F at 2012. Paternity identification was carried out total 26 offspring in C local government of 2012 and then confirmed 100% agreement of its analysis. In conclusion, the results obtained the possibility of mass production of elite cow embryos as well as offspring by OPU derived embryo production system, of which could be decreased the required time of genetic improvement.

OPU 유래 한우 수정란 생산 및 이식

진종인,최병현,김성수,조현태,선두원,임현태,이정규,민찬식,공일근 사단법인 한국동물생명공학회 2014 한국동물생명공학회지 Vol.29 No.3

This study was carried out to establish the system of OPU derived embryo production, management of recipientsas well as offspring production. OPU derived embryo production system was carried out of aspiration of immatureoocytes 2 times per week, total 24 times for 3 months by an ultrasonographic guided follicular aspiration system andthen produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture system. This work wascollected total 13,866 oocytes, average 8.2 ± 4.5 oocytes per session and 8,170 G1 + G2 grade oocytes, average 4.8oocytes per session by 1,692 times session of total 71 donors for 4 years from 2010 to 2013. The rate of cleavageand blastocyst developmental competence were obtained 11,825 (85.3%) and 5,032 (36.3%) that was 7.0 ± 3.8 cleavedembryos and 3.0 ± 2.5 blastocysts per session. OPU derived embryo transfer were taken place in 2, 4, 6 and 7 localgovernments at 2010, 2011, 2012 and 2013 for 4 years and pregnancy rate were obtained 41.2, 43.9, 46.5 and 49.7%in each years. It means that pregnancy rate was continuously improved according of every year for 4 years. Pregnancyrate was significantly different according to individual local government in which was 62.7% in B, but 24.2% in Fat 2012. Paternity identification was carried out total 26 offspring in C local government of 2012 and then confirmed100% agreement of its analysis. In conclusion, the results obtained the possibility of mass production of elite cowembryos as well as offspring by OPU derived embryo production system, of which could be decreased the requiredtime of genetic improvement.

OPU 유래 수정란의 대량생산을 위한 고능력 공란우 반복사용 가능성에 관한 연구

진종인,최병현,김성수,박번영,이정규,공일근 사단법인 한국동물생명공학회 2015 한국동물생명공학회지 Vol.30 No.3

This study was designed to know the possibility in repeat uses of elite donor cows for getting mass production of OPU-derived embryo production (OPU-IVP). Ultrasound transvaginal ovum pick-up (OPU) performed in 6 Korean native cows was aged 4 to 10 years old. The aspiration of immature oocytes for OPU derived embryo was carried out 2 times per week, and OPU-IVP of 1st period was carried out 22∼48 sessions from each donors. And the break time for OPU-IVP of 2nd period after 1st OPU from each donors were 2∼25 months. The OPU-IVP of 2nd period each donors conducted total 15∼65 times for 2∼8 months by an ultrasonographic, was guided follicular aspiration system. The average numbers of collected oocytes, grade 1 + grade 2(G1+G2) oocytes and cleavage embryo from 1st period OPU-IVP were significantly differences between donors (p<0.05). Total collected oocytes of donor D were significantly higher compared with donors of A, B, C, E and F (average 17.0 per session vs. 11.2, 10.1, 8.5, 10.2 and 9.6; p<0.05) and also oocytes of G1+G2 were significantly higher compared with r A and D and subsequently to donors of B, C, E and F (average 7.9 and 8.5 per session vs. 5.0, 2.7, 6.0 and 1.6; p<0.05). Cleavage rate of donor D was significantly higher compared with donors of A, B, C, E and F (average 13.1 per session vs. 10.1, 9.1, 6.9, 8.9 and 6.7; p<0.05). The average numbers of OPU-IVP for 1st period was significantly higher from donors of B, D and E than those from donors of A, C and F (average 6.5, 7.1 and 6.5 per session vs. 3.5, 4.2 and 2.8; p<0.05). The possibility investigation of 2nd OPU-IVP was carried out after 2∼25 months rest periods from 1st period OPU session. Total average numbers of collected oocytes, cleavages and blastocyst development rates were significantly higher from 1st period OPU compared with 2nd period one (p<0.05). The OPU-IVP efficiency by break for more embryo production from elite cow was analysis comparing without rest of donor A, under 6 months rest period as B and over 6 months rest period as C and then the average numbers of collected oocytes, cleavages and blastocysts were significantly higher from A group (11.8, 9.5 and 5.2 per session) than those from B and C groups (7.9, 6.2 and 2.6 vs. 9.2, 7.5 and 3.9, p<0.05), and also C group was significantly higher than B group. In conclusion, 1st period OPU-IVP was more efficient compared with 2nd period repeated uses of donor, and the break times for additional production of embryo on donor were needed more than over 6 months after 1st period OPU-IVP. This repeating uses of elite donor cows given more emphasis for getting the opportunity on mass production of elite cow OPU-IVP embryo should be increased G1+G2 possibility of genetic improvement of livestock within short period.

OPU(Ovum Pick-Up) 채란기간이 난자 및 수정란 생산에 미치는 영향

진종인,권태현,최병현,김성수,조현태,공일근 韓國受精卵移植學會 2010 한국동물생명공학회지 Vol.25 No.1

본 연구에서는 OPU를 통한 체내 유래 난자를 이용한 수정란 생산 시 1개월에서 6개월까지의 연구 기간에 따른 난포 생성수, 난자 회수율, 난자 등급율, 배반포 생성률을 분석하여 공란우의 활용 기간에 관하여 조사하였다. 1. 채란 기간에 따른 난포 생성수는 1개월에서 5개월까지의 난포 생성수는 , , , , 개로 유의적인 차이가 없었으나, 6개월째에는개로 급격하게 줄어든 것을 알 수 있었다. 2. 채란 기간에 따른 난자 회수개수는 1개월에서 3개월까지는 This study was performed to identify the optimal timing for oocyte donor replacement during OPU procedure. OPU was carried out to collect oocytes from every donor at an interval of days (2 times a week). The collected oocytes were matured in vitro in TCM-199 supplemented with 10% FBS, 10 mg/ml of FSH and 1 mg/ml of estradiol for 24 h. After 24 h of exposure to sperm, the presumptive zygotes were cultured in CR1aa medium supplemented with 4 mg/ml of BSA for 3 days before being changed to CR1aa medium with 10% of FBS for another days. The mean numbers of retrieved oocytes were remained constantly up to 3 months (, , ), but significantly decreased at over 4 to 6 months (, , ) (p<0.05). The blastocyst development potential was also very similar rate from 1 to 3 months (37.2%, 40.4% and 44.6%), but significantly decreased from 4 to 6 months (24.8%, 29.3% and 28.6%, respectively) (p<0.05). The production of OPU derived embryos in periods of 1 to 3 months (, and ) were significantly higher than those in 4 to 6 months (, and , respectively) (p<0.05). In conclusion, the efficient periods for the production of OPU derived embryos was until 4 months, twice per week to produce over 64 transferable embryos and then replace new donor after 3 months use. The best replacement time is 3 months and could be maximized production of OPU derived embryos.

반도체 패키징 TSV 측정 기술

진종한(J. Jin),김재완(J. W. Kim),김종안(J. -A. Kim),강주식(C. -S. Kang),이성현(S. H. Lee) 한국정밀공학회 2011 한국정밀공학회 학술발표대회 논문집 Vol.2011 No.6월

간호대학생의 투지(Grit), 학업탄력성 및 심리적 안녕감

진종임(Jin, Jong Im),김남초(Kim, Nam Cho) 한국간호교육학회 2017 한국간호교육학회지 Vol.23 No.2

Purpose: This study was conducted to investigate the relationship among grit, academic resilience, and psychological well-being among nursing students. Methods: The participants were 271 nursing students, enrolled in C university. Data were collected from September 5 to 30, 2016 and analyzed using descriptive analysis, t-test, ANOVA, Pearson correlation coefficients, and stepwise multiple regression with the SPSS/WIN 21.0 program. Results: The mean scores for academic resilience were above average. The mean score for grit and psychological well-being presented an appropriate level. Psychological well-being in nursing students was significantly correlated with academic resilience (r=.65, p<.001) and grit (r=.52, p<.001). The significant predictors of psychological well-being for nursing students were academic resilience (β=.47, p<.001), grit (β=.26, p<.001), and major satisfaction (β=.20, p<.001) which explained 53.1% of the variance in psychological well-being. Conclusion: The results indicate that it is necessary to develop grit and academic resilience enhancement programs to improve the psychological well-being of nursing students.

OPU 유래 수정란의 대량생산을 위한 고능력 공란우 반복사용 가능성에 관한 연구

진종인,최병현,김성수,박번영,이정규,공일근 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.3

This study was designed to know the possibility in repeat uses of elite donor cows for getting mass production of OPU-derived embryo production (OPU-IVP). Ultrasound transvaginal ovum pick-up (OPU) performed in 6 Korean native cows was aged 4 to 10 years old. The aspiration of immature oocytes for OPU derived embryo was carried out 2 times per week, and OPU-IVP of 1st period was carried out 22∼48 sessions from each donors. And the break time for OPU-IVP of 2nd period after 1st OPU from each donors were 2∼25 months. The OPU-IVP of 2nd period each donors conducted total 15∼65 times for 2∼8 months by an ultrasonographic, was guided follicular aspiration system. The average numbers of collected oocytes, grade 1 + grade 2(G1+G2) oocytes and cleavage embryo from 1st period OPU-IVP were significantly differences between donors (p<0.05). Total collected oocytes of donor D were significantly higher compared with donors of A, B, C, E and F (average 17.0 per session vs. 11.2, 10.1, 8.5, 10.2 and 9.6; p<0.05) and also oocytes of G1+G2 were significantly higher compared with r A and D and subsequently to donors of B, C, E and F (average 7.9 and 8.5 per session vs. 5.0, 2.7, 6.0 and 1.6; p<0.05). Cleavage rate of donor D was significantly higher compared with donors of A, B, C, E and F (average 13.1 per session vs. 10.1, 9.1, 6.9, 8.9 and 6.7; p<0.05). The average numbers of OPU-IVP for 1st period was significantly higher from donors of B, D and E than those from donors of A, C and F (average 6.5, 7.1 and 6.5 per session vs. 3.5, 4.2 and 2.8; p<0.05). The possibility investigation of 2nd OPU-IVP was carried out after 2∼25 months rest periods from 1st period OPU session. Total average numbers of collected oocytes, cleavages and blastocyst development rates were significantly higher from 1st period OPU compared with 2nd period one (p<0.05). The OPU-IVP efficiency by break for more embryo production from elite cow was analysis comparing without rest of donor A, under 6 months rest period as B and over 6 months rest period as C and then the average numbers of collected oocytes, cleavages and blastocysts were significantly higher from A group (11.8, 9.5 and 5.2 per session) than those from B and C groups (7.9, 6.2 and 2.6 vs. 9.2, 7.5 and 3.9, p<0.05), and also C group was significantly higher than B group. In conclusion, 1st period OPU-IVP was more efficient compared with 2nd period repeated uses of donor, and the break times for additional production of embryo on donor were needed more than over 6 months after 1st period OPU-IVP. This repeating uses of elite donor cows given more emphasis for getting the opportunity on mass production of elite cow OPU-IVP embryo should be increased G1+G2 possibility of genetic improvement of livestock within short period.