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단백질 수준 및 DL - Methionine 첨가가 초생추의 성장율 및 혈액 Cholesterol 함량에 미치는 효과
지규만,육종융,이영상 한국축산학회 1969 한국축산학회지 Vol.11 No.2
This experiment was carried out to investigate the effects of protein levels and DI-methionine supplementation on the plasma cholesterol levels and the growth rate of chicks fed semi-purified diets containing soybean oil meal and perilla oil meal as dietary protein sources. One hundred male chicks of 10 day old S.C.W. Leghorn were alloted into 10 lots. The treatments imposed were three different levels of protein (15, 20 and 25%) and three levels of DL-methionine supplementation (0, 0.1 and 0.2%) as 3×3 factorial design. Fish meal was added at the level of 12% to compare its effect on various items with vegetable protein source only at optimal protein level (20%). The experimental results obtained were as follows: 1) The growth rate was highly significantly (P$lt;0.01) improved as the level of protein was increased. But there was no significant difference between the levels of methionine. Also, highly significant improvement was appeared in growth rate by fish meal fed chickens. 2) Highly significant difference was observed in the amount of feed-intake by the chicks fed the diet containing different level of dietary protein. Chicks fed 15% protein consumed significantly (P$lt;0.05) less amounts of diet than those fed 20 or 25% protein level S. Dietary protein level of either 20 or 25% did not result any significant difference in the feed consumption, 3) Feed efficiency was significantly (P$lt;0.01) improved as the level of protein was increased and also by supplementing excess amount of DL-methionine. Fish meal increased not only feed consumption amount but also improved feed efficiency as 2.47 compared with 3.11 of 0.1% DL-methionine supplementation group. 4) Plasma cholesterol levels were significantly (P$lt;0.01) increased when chicks were fed diets containing 15% protein level. Chicks fed the diet of 25% protein level showed the lowest level of plasma cholesterol. In comparison with 0.1% and 0.2% of DL-methionine supplementation groups in each protein level, feeding of the excess amount of methionine significantly (P$lt;0.01) depressed cholesterol levels in chicks fed 15 and 20% protein diet. No difference was found in plasma cholesterol level in fish meal lot. 5) Although, no significant difference was observed in metabolizability of energy between chicks of 0.1 and 0.2% DL-methionine supplementation groups, the former showed higher values than those of the latter. Chicks fed 25% protein diet showed the lowest value of metabolizable energy (M.E.) (P$lt;0.01). Fish meal increased the valve of M.E. with no significance. 6) The percentages of Nitrogen retention was the lowest in groups of 25% protein level (P$lt;0.01) but showed no difference between each level of methionine supplementation or between each treatment of 20% protein diet.
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지규만,Donald W. Bannister 한국가금학회 1980 韓國家禽學會誌 Vol.7 No.2
가금의 생화학적기전에 대한 연구는 아직도 다른 분야의 연구에 비해 많이 뒤떨어져 있지만, 중간대사의 주요과정들이 포유동물의 것과 본질적으로 매우 유사하다는 정도는 알려져 있다. 가금과 포유동물 사이의 중요한 생화학적 차이점들에 대해서 최근의 총설(Pearce, 1977)에서 자세히 다뤄졌으며 여기서는 그중 두 가지에 대해서만 간단히 언급코자한다. (중략)
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Paired - Feeding 방식에 의한 대두단백질 , 카제인 및 어유의 급여가 병아리의 혈액과 간내 콜레스테롤 함량에 미치는 영향
지규만,최인숙 한국축산학회 1995 한국축산학회지 Vol.37 No.2
Two feeding trials, with the same diets, were conducted for periods of 4 weeks each to investigate possible interrelationships in control mechanisms of blood cholesterol level by casein and fish oil in young chicks. Comparisons were made between influences of dietary casein vs. soy protein, fish oil(EPA 24.8%, DHA 10.1%) vs. com oil and cholesterol added at levels of 0 vs. 0.3% on plasma and liver cholesterol. The chicks in Exp. 1 were fed the diets ad libitum while those in Eop. 2 were paired-fed to equalize the amount of dietary intake in order to remove any effects arising from differences in feed intake. Chicks consuming the casein in Exp. 1 showed reduced feed intake and body weight gain compared to those fed soy protein diet(P$lt;0.01). The same response in body weight gain was obtained in chicks of Exp. 2. In Exp. 1, casein intake increased total cholesterol in the plasma and liver(P$lt;0.05), mainly due to high levels of plasma low density lipoprotein cholesterol(LDL-C). Although fish oil ingestion did not affect plasma cholesterol levels elevated by casein feeding (Exp. 1), paired-feeding of the casein + fish oil diet(Exp. 2) resulted in the same level of total plasma cholesterol as that obtained by the soy protein +- fish oil by lowering the LDL-C. The reduction of casein-induced high plasma cholesterol levels by addition of hsh oil is not enough to confirm the existence of an interrelationship between the control mechanisms of casein and fish oil. Further research needs to be conducted to confirm the observations in the present study.
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계란의 콜레스테롤 함량과 지방산의 조절 - 현재와 전망
지규만 한국가금학회 2004 韓國家禽學會誌 Vol.31 No.1
현대인이 섭취하고 있는 식품 중에서 계란의 콜레스테롤처럼 많은 연구자의 관심을 끌었던 성분도 많지 않을 것이다. 콜레스테롤 섭취와 심장혈관계 질환과의 연관성에 대한 우려는 계란이 영양적으로 매우 우수한 식품임에도 불구하고 그 소비에 막대한 지장을 주고 있다.(중략)
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고온 환경조건의 육용종계에서 비타민 섭취에 의한 난각질 저하 방지 효과
지규만,정만기 한국가금학회 2003 한국가금학회 심포지움 Vol.- No.-
Heat stress환경의 육용 종계에서 사료에 첨가한 비타민 C(200 ㎎/㎏)와 비타민 E (250 ㎎/㎏)가 난각 품질 및 경골 강도에 미치는 영향을 확인하고자 실험을 실시하였다.<br/> 강제 환우에서 회복된 83주령의 Ross품종 육용 종계 160수를 4처리 4반복 10수씩 개별 케이지에 수용 한 뒤, 10일간의 적응 기간을 둔 뒤, 3주간에 걸쳐 32 ℃에서 지속적으로 온도를 유지하면서 사료 섭취량, 산란율, 폐사율, 난중, Haugh unit, 난각의 품질, 경골 파괴강도 그리고 혈액 중의 혈구 세포 등을 조사하였다.<br/> Heat stress는 폐사율, 산란율, 난중, 그리고 Haugh unit를 감소시키는 경향이 있었으나 통계적으로 유의하지는 않았다. 난각의 SWUSA와 압축 파괴강도는 비타민 C/E 첨가구에서 2,3주차에 무첨가구보다 유의하게 높았으나 (P<0.05), 비타민 C 또는 E 첨가구 사이에서는 유의차가 없었다. Heterophil과 lymphocyte의 숫자는 heat stress 동안에 각각 증가 또는 감소하였다. 처리구간에는 비타민 C/E 첨가구가 H/L/ratio 수준이 가장 낮았으며, 무첨가구가 가장 높은 경향을 나타내었다. 경골의 파괴강도는 비타민 C 첨가구에서 가장 높았다(P<0.05). 혈액 중의 비타민 C 농도는 비타민 C첨가구와 비타민 C/E 첨가구에서 각각 12.73 g/ml과 8.23 g/ml으로 높았다 (P<0.05). 비타민 E 첨가구와 비타민 C/E 첨가구에서 혈액 중 비타민E농도가 유의하게 높았다 (P<0.05). Corticosterone 농도는 무첨가구에서 5.97 g/ml으로 통계적으로 유의하게 높았다(P<0.05).
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