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노랑초파리의 GD STERILITY 에 관한 온도 및 AGE 의 영향에 관한 연구
이혜영,지관자 한국유전학회 1987 Genes & Genomics Vol.9 No.4
An experiment was carried out to investigate the influence of temperature and aging on GD sterility from reciprocal crosses with π_2strain and Canton-S strain of Drosophila melanogaster. It was found that the GD sterility traits were observed display a characteristic reciprocal cross effect by low egg production and strongly depend on female aging effect and sensitive in various temperature. It was concluded that the GD sterility was induced by high and narrow range of temperature 25℃-29℃ and age a female produced high egg production.
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쥐 유방암종에서 흉선세포 융합에 의한 전이능 증가 기작에 관한 연구
김철우,지관자,김종재 한국유전학회 1993 Genes & Genomics Vol.15 No.2
We have obtained three hybrid cell lines- FA4, FB4 and FB6 - through hybridization process between rat NMU mammary carcinoma cell line and allogeneic thymocytes. Those hybrid cell lines were inoculated with matrigel into the interscapular subcutaneous tissue in newborn Sprague-Dawley rats. About 5-14 weeks later, metastatic nodules were developed in the lungs and axillary lymph nodes among the animals, which were inoculated by FB6 or transplanted subsequently by those tumor masses. The expression of Thy 1.1 and T cell antigen was higher in FB6-12, one of the established cell lines which were gained from metastatic tumor lesions by inoculation of FB6, than in other cell lines. Also we have obtained FB6-HM subclone which was selected as a highly metastatic clone from FB6 by limiting dilution method. The evidence of hybridization between NMU and thymocytes was disclosed by the fact that FB6-HM clone showed a positive reaction against polyclonal rabbit sera raised by repeated immunization with S-D rat thymocytes. CD44 molecule, which has been known that the degree of its expression in the neoplastic epithelial cells was positively correlated with the increased metastatic potential, was also more expressed in FB6-12 than in any other cell lines. The results of this study strongly supported the hypothesis that epithelial tumor cells could gel metastatic potential through hybridization with lymphoreticular lineage cells. Additionally, by establishing the metastasizing mammary carcinoma in Sprague-Dawley rat model, we could perform proper experiments on the study of mechanism of hematogenous and lymphatogenous metastasis in cancer.
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이상범,남주현,김용만,지관자,김종혁,목정은,김영탁 대한부인종양 콜포스코피학회 2000 Journal of Gynecologic Oncology Vol.11 No.1
Until recently, cytogenetic studies failed to identify any landmark chromosomal aberrations associated with cervical carcinomas, Recent comparative genome hybridization(CGH) studies have, however, demonstrated that one of the most consistent chromosomal abnormality that marks the transition of carcinoma in situ (CIS) of the cervix to invasive carcinoma is the gain of specific chromosome 3q sequences. Although HPV infection has been demonstrated to be a most important initiating event in the development of most cervical cancers, other additional genetic events are also required for eventual development or progression of invasive cancer. The genetic alterations in cervical cancer were investigated by CGH method using fresh frozen specimens. CGH is based on two-color in situ hybridization where genomic tumor DNA is labeled with fluorochrome (FITC) and a normal reference genome is labeled with fluorochrome (Rhodamine) by nick translation. Following co-hybridization of the deferentially labeled genomes to normal reference metaphase chromosomes, Computer Assisted Image Analyzer interprets multicolor fluorescence. The volumetric change in the dual color is typically detected as gain or loss of the DNA sequences in specific region of chromosome. In this study, the pattern of chromosomal aberrations in cervical cancer was not similar to that reported previously by other authors. Overall chromosomal aberration was observed in 46.2%(12/26 cases). The gain of chromosome 5, 11q, 15q, and 17q was most frequent. In deletion, 1q loss was most frequent. There was some cell to cell variability of CGH results in a same tumor sample. The results await careful interpretation and there are several possible reasons of such difference. First reason is a possibility of racial difference in the pattern of chromosomal alteration necessary for cervical carcinogenesis and second one is a possibility of faulty analysis by either improper standardization of computer software or heterogeneity of specimen due to normal cell contamination. However, from the finding that there was no chromosomal aberration in all myometria used for normal control, threshold of normal fluorescence profile in our CGH seems to be reliable. The genetic studies on cervical cancer by this CGH technique should provide new insights into the molecular pathogenesis of lower genital tract cancer and allow for more logical and targeted approach to the cervical cancer management.
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