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현학철,주충노,Hyun, Hak-Chul,Joo, Chung-No 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.4
쥐 간의 aldehyde oxidase (ALO)와 xanthine oxidase (XO)의 활성 분포와 이 효소들에 미치는 인삼사포닌 분획의 영향을 조사하였다. ALO나 XO, 다같이 주로 시토졸 분획에 존재하였으며 ALO의 경우에는 미토콘드리아와 미크로좀분획에서도 약간의 활성이 관찰되었으나, XO 활성은 마토콘드리아나 미크로좀에서 전혀 관찰되지 않았다. ALO, XO 다같이 적당량 ($10^{-7}-10^{-3}%$)의 인삼 사포닌 존재하에서 활성화되었으며, 인삼 사포닌의 농도가 $10^{-5}%$일때 ALO나 XO 모두 최대활성을 나타내었다. 이것은 본 연구실에서 관찰한 적당량의 인삼 사포닌이 대부분의 효소를 비특이적으로 활성화한다는 실험결과와 잘 일치한다. The distribution of aldehyde oxidase (ALO) and xanthine oxidase (XO) in rat liver was investigated and effect of saponin fraction of panax ginseng (C.A. Meyer) roots on the above enzymes was examined. Both ALO and XO were present exclusively in the cytosol fraction and a little ALO activity was detected in mitochondria and microsomal fraction, but no XO activity was detected either in mitochondria or in microsomal fraction. Both ALO and XO were stimulated in the presence of ginseng saponin fraction ($10^{-7}-10^{-3}%$) and they showed their maximum activities at the saponin concentration of $10^{-5}%$. This is in good agreement with the previous results obtained with other enzymes in this laboratory that moderate amounts of ginseng saponin stimulates most enzymes nonspecifically.
쥐 간 세포의 Aldehyde oxidase , Xanthine oxidase 의 활성분포와 이들 효소에 미치는 인삼 사포닌 분획의 영향
현학철,주충노 ( Hak Chul Hyun,Chung No Joo ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.4
The distribution of aldehyde oxidase (ALO) and xanthine oxidase (XO) in rat liver was investigated and effect of saponin fraction of panax ginseng (C.A. Meyer) roots .on the above enzymes was examined. Both ALO and XO were present exclusively in the cytosol fraction and a little ALO activity was detected in mitochondria and microsomal fraction, but no XO activity was detected either in mitochondria or in microsomal fraction. Both ALO and XO were stimulated in the presence of ginseng saponin fraction (10^(-7)-10^(-3)%) and they showed their maximum activities at the saponin concentration of 10^(-5)%. This is in good agreement with the previous results obtained with other enzymes in this laboratory that moderate amounts of ginseng saponin stimulates most enzymes nonspecifically.