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주완택,민경수,박노동,Ju, Wan-Taek,Min, Kyung-Soo,Park, Ro-Dong 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.4
유색보리 4품종과 보통보리 4품종 종실의 종피에 함유되어 있는 색소의 특성을 분석하여 해당작물의 신품종 육성시 우수계통 선발에 유용한 자료를 제공코자 하였다. 유색(검정)쌀보리인 Ab2231을 표준시료로 하여 실험한 결과 에탄올의 농도가 80%일 때 색소추출 효율이 양호하였다. 80% 에탄올에 1.0% 염산을 첨가한 것이 추출효율이 가장 좋았다. 추출시간에 따른 추출효율성을 조사한 결과 1시간 추출이면 충분하였다. 추출된 색소의 안정성을 유지하기 위하여 첨가시키는 산의 적정농도를 알아본 결과 1.0% HCI을 첨가한 농도에서 가장 낮은 색소량 감소율이 나타났다. 각 보리 품종 시료에서 추출한 색소의 흡광스펙트럼을 조사하였던 바, Ab2231의 그것은 535 nm에서 최대흡광파장을 나타내어 안토시아닌계 색소로 판단되었으며, 호계4호의 그것은 안토시아닌계 이외의 색소를 주로 함유하는 것으로 보였다. 한편, 추출된 색소의 특성에 따라 유색보리의 품종은 Ab2231와 모찌무기로 대표되는 쌀보리 그룹과, Lion과 호계4호로 대표되는 겉보리 그룹으로 분류되었다. The characteristics of the pigments extracted from grains of the colored barley varieties Ab2231, Mozzimugi, Lion, and Hogye 4 were studied. Extraction of the pigments from powder (80 mesh) of the barley grains was most efficient in 80% ethanol containing 1.0% HCI for 1 hr at $60^{\circ}C$. The pigments were also stable in 80% ethanol containing 1.0% HCI. Depending on the absorption spectra, maximal peak wavelength, and the ratio of $A_{440}/A_{max}$, the varieties were separated into two groups, colored naked barley (Ab2231 and Mozzimugi) and colored covered barley (Lion and Hogye 4).
Streptococcus mutans Ingbritt sucrose-glucan glucosyltransferase특성과 그 활성에 미치는 키틴 유도체들의 효과
주완택 ( Wan Taek Ju ),지명심 ( Myeong Sim Ji ),박노동 ( Ro Dong Park ) 한국응용생명화학회(구 한국농화학회) 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.3
Sucrose-glucan glucosyltransferase (Gtf) is an importantenzyme involved in the cavity formation process where insolubleglucan is synthesized. In this study, we purified Gtf from Streptcoccusmutans Ingbritt through ammonium sulfate precipitation, SephadexG-150, CM-Sephadex, and DEAE-Sephadex column chromato-graphies. A 13-fold of purification was achieved with a total yieldof 6.3%. The apparent molecular mass of the enzyme was determinedto be 66kDa on sodium dodecyl sulfate-polyacrylamide gelelectrophoresis (SDS-PAGE). The optimal pH and temperaturewere established to be 6.0 and 40oC, respectively. The enzymeactivity could be inhibited to 22-59% by 1mM Hg2+, Cu2+ andAl3+, and to 68% by 1mM EDTA. It was also inhibited 40% by2mM xylitol and 35-45% by 0.05% soluble chitosan, glycolchitosan, and glycol chitin. This is the first report to reveal theinhibition effect of chitin derivatives on Gtf activity, which maybe further applicable to develop gargles to overcome cavity.
한국산 뽕나무 잎 12종의 플라보노이드에 대한 UPLC-DAD-QTOF/MS 분석
주완택 ( Wan-taek Ju ),권오철 ( O-chul Kwon ),김현복 ( Hyun-bok Kim ),성규병 ( Gyoo-byung Sung ),김용순 ( Yong-soon Kim ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-
Flavonoids are a large group of polyphenolic compounds found in fruits, vegetables, and herbs. Plants of the genus Morus are known to be rich in flavonoids, including quercetin 3-(6-malonylglucoside), rutin, isoquercitin, cyanidin 3-rutinoside, and cyanidin 3-glucoside. These compounds are known to have potential antioxidant properties and probable roles in preventing oxidative stress-associated diseases. In this study, the total flavonoids in leaves of 12 varieties of Korean mulberry (Morus alba L.) were determined. Seventeen flavonoids were isolated and analyzed using ultra-performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS). To determine the flavonoid contents, HPLC analysis was performed on these 17 flavonoids. The total flavonoid contents of the 12 varieties of mulberry leaves ranged from 748.5 to 1297.9 mg, with the highest obtained from the Cheong Su variety (1297.9±112.0 mg). Among the 17 flavonoids analyzed, quercetin 3-O-rutinoside (rutin) and quercetin 3-O-glucoside (isoquercitrin) had highest contents in the Cheong Su variety. Furthermore, the Dae Dang Sang variety gave the highest quercetin 3-O-rutinoside (rutin) content among the mulberry leaves investigated, at 425.5±45.9 mg. Major flavonols from Dae Dang Sang were detected by UPLC-DAD-QTOF/MS. A total of 17 flavonoid compound peaks were identified in the analysis time range of 5-40 min, all of which were kaempferol and quercetin glycosides. It has been observed that seven of the 17 compounds identified are reported in mulberry leaves for the first time, and further research will be. This study could provide basic information for mulberry breeding.
키틴, 키토산, 유카추출물이 오이 모잘록병의 생물학적 방제 효과 증진에 미치는 영향
김성모 ( Sung Mo Kim ),주완택 ( Wan Taek Ju ),송용수 ( Yong Su Song ),김영주 ( Young Ju Kim ),정우진 ( Woo Jin Jung ),박노동 ( Ro Dong Park ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.1
To test the effect of biological control on the dampping-off (Rhizoctonia solani) of cucumber, chitin (CTN), chitosan (CSN), yucca extract (YE), and microorganism formulations [Bacillus subtilus (BIO), Trichoderma harzianum GBF-0208 (TRI), and Bacillus subtilus GB-0365 (CIL)] were applied to the cucumber seedlings in an environment-controlled chamber. Growth characteristics and the incidence of dampping-off (IDO) were investigated in the treated cucumber seedlings for 9 days after inoculation of R. solani. When treated with mixture of 1% yucca extract and 1% chitin, growth of cucumber seedlings was the highest. IDO decreased less than 50% in the plants treated with the mixture of chitin, chitosan, and yucca extract, while IDO was over 50% in the single treatment of chitin, chitosan, and yucca extract. Especially, yucca extract decreased IDO significantly. IDOs were 22.2% in the plants treated with BIO+YE and 25% with CIL+YE, suggesting the synergistic effect of YE with BIO and CIL. All these results indicated that chitin, chitosan, and yucca extract showed a bio-control effect against the cucumber dampping-off R. solani disease and the application of the microorganism formulations together with them was also proven the synergistic effect on controlling the cucumber dampping-off R. solani disease.
발효 뽕잎의 배양조건에 따른 알파-글루코시다제 저해활성
권오철 ( O-chul Kwon ),주완택 ( Wan-taek Ju ),김현복 ( Hyun-bok Kim ),성규병 ( Gyoo-byung Sung ),김용순 ( Yong-soon Kim ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-
Mulberry (Morus alba L.) has widely distributed from the tropical, sub-tropical, and temperate regions of the world. It has long been used as a traditional medicine and foods in Korea, China, and Japan. Mulberry leaves possess the biologically active compounds such as 1-deoxynojirimycin (DNJ), rutin, quercetin, isoquercitrin, chorogenic acid, and caffeic acid. In particular, mulberry leaves have rich in iminosugar (N-containing sugar) including DNJ, and these compounds have a strong inhibitory effect for α-glucosidase. α-glucosidase inhibitor is usually used to prevent or medical care of type II diabetes. These inhibitors combine with intestine α-glucosidase and blocking the absorption of postprandial blood glucose. Researchers have reported that the human and rat small intestinal maltase and sucrase were strongly inhibited by mulberry leaf extract. These results were confirmed that the inhibition of maltase and sucrase was mainly due to the presence of DNJ. Mulberry leaves containing DNJ have been recognized as a potentially important source for prevent or treat hyperglycemia. However, DNJ content of natural mulberry leaf are as low as 0.1%. Thus, the most effective method for increasing α-glucosidase inhibitory activity with the DNJ high-production is needed. In this study, we investigated the influence of α-glucosidase inhibitory activity according to different pH values (6-9) and inoculation amounts (0.1-0.5%) when Bacillus subtilis cultured on mulberry leaf powder media. We confirmed that α-glucosidase inhibitory activity was difference according to culture conditions of different pH values, inoculation amounts, and fermentation times. The results of mulberry leaf fermentation according to pH values and inoculation amounts were shown that the optimal conditions for α-glucosidase inhibitory activity were defined as pH 7 and 9, inoculation amount 0.4%, and incubation until 2 to 4 days. These results can be provided a basic data for the optimal culture conditions increasing α-glucosidase inhibitory activity from mulberry leaf fermentation.
홍성현 ( Seong Hyun Hong ),조경현 ( Gyung Hyun Jo ),주완택 ( Wan Taek Ju ),박노동 ( Ro Dong Park ) 한국키틴키토산학회 2010 한국키틴키토산학회지 Vol.15 No.2
매미 허물로부터 화학적 처리법으로 키틴을 분리하고 그 물리화학적 특성을 조사하였다. 얻어진 키틴은 백색의 분말 형태였으며 그 수율은 31.7%였다. 매미 키틴은 30℃에서 진한 염산 가수분해 반응으로 올리고당으로 분해되었으며, 반응시간에 따라 키틴의 분해율은 증가하여 24시간에는 44.0%의 분해율을 기록하였다. GlcNAc1-3의 함량 비율은 반응 0.5시간에 66.5%, 반응 12시간에는 84.4%로 증가하였다. 반응 24시간에는 단당 GlcNAc의 함량이 79.4%를 차지하였으며, 4당 이상은 검출되지 않았다. 매미허물 키틴은 게, 새우 키틴과 같은 형태의 FT-IR 스펙트럼 양상이 나타나 α-chitin으로 확인되었고, 아세틸화도는 88.1%로 조사되었다. 매미 허물 키틴의 X-선 회절 분석결과 2θ = 9.4˚, 19.4˚에서 면간격이 9.39 Å와 4.60 Å로 각각 측정되었으며, 결정화도 및 결정크기의 값은 각각 81.9%, 4.6 nm로 α-chitin인 게, 새우 키틴의 특성치와 유사하였다. In this study, the physicochemical properties of the chitin isolated from cicadae (Platypleura kaempferi) periostracum was investigated. The prepared chitin was white flake form and the yield was 31.7%. The chitin was degraded into oligosaccharide during acid hydrolysis at 30℃ with concentrated HCl. The hydrolysis rate was 6.8% only after 0.5 h reaction, but increased to 44.0% after 24 h reaction. In distribution of the oligosaccharides in the hydrolyzates, ratio of GlcNAc1-3 content increased from 66.5% after 0.5 h reaction to 84.4% after 12 h reaction. The ratio of monomer GlcNAc content was 79.4% after 24 h reaction and no oligosaccharides bigger than GlcNAc4 was detected. It is found that cicadae periostracum chitin belongs to α-chitin resulting from the FT-IR spectra similar with those of crab and shrimp chitins. In X-ray diffractometer, two peaks were exhibited at 2θ = 9.5˚ (interplanar distance: 9.36Å) and 2θ = 19.4o(4.58Å). The crystallinity index and crystal size of the chitin were determined to be 81.9% and 4.6 nm, respectively, which are similar with those of crab and shrimp chitins, again confirming that cicadae periostracum chitin belongs to α-chitin. The degree of acetylation of cicadae periostracum chitin was calculated to be 88.1%.