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적조원인종인 Prorocentrum minimum의 광도에 따른 생화학적 조성의 변화
조진하,이택견,신경순,이우성,장만 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.4
적조원인종인 Prorocentrum minimum의 생화학적 조성의 변화에 대한 광도의 영향을 조사하였다. 성장조건은 14:10h(L:D)로 설정하였으며, 15, 50, 120μ㏖·m^-2·s^-1의 광도범위에서 실험을 수행하였고, 지수함수기에 세포를 모아 생화학적 구성성분을 분석하였다. 50μ㏖·m^-2·s^-1 이상에서는 성장유형이 거의 유사하였고, 세포내 단백질, 탄수화물 및 지질의 양은 각각 120, 50, 15μ㏖·m^-2·s^-1에서 가장 많이 존재하는 것으로 나타났다. 단백질/탄수화물의 비율과 MGDG/DGDG의 비율은 50μ㏖·m^-2·s^-1에서 가장 높았으며 photosystem Ⅱ의 활성도는 120μ㏖·m^-2·s^-1 이상의 광도에서 성장한 세포에서 가장 낮았다. 따라서 50μ㏖·m^-2·s^-1의 광도에서 성장한 세포가 세포활성도 및 광합성능이 가장 높은 것으로 관찰되었다. Effects of light intensity on the biochemical composition of Prorocentrum minimum, a dinoflagellate causing red tide, were examined. Cultures were grown under a 14:10h light: dark(L:D) regime at three light intensity ranging from 15 to 120μ㏖·m^-2·s^-1 and harvested at late-logarithmic phase for analysis of biochemical composition. Growth pattern was similar to over the 50μ㏖·m^-2·s^-1. The highest levels of carbohydrate, protein and lipid were present in the cells from cultures grown at 120, 50 and 15μ㏖·m^-2·s^-1, respectively. The protein/carbohydrate ratio and MGDG/DGDG ratio was highest in cells from culture grown at 50μ㏖·m^-2·s^-1. The activity of photosystem Ⅱ of the culture grown 120μ㏖·m^-2·s^-1 was lower than others. Therefore the cells grown 50μ㏖·m^-2·s^-1 represented highest cellular activity and photosynthetic activity.
조진하,배은영,이태경,김명현,이승웅,김병수,임치환 한국약용작물학회 2016 한국약용작물학회지 Vol.24 No.2
Background: Sanguisorba officinalis has been used in traditional Asian medicine owing to its beneficial effects on various diseases. The purpose of this study was to evaluate the effect of S. officinalis on the antioxidant system of Streptozotocin (STZ) and Alloxan (ALL) induced diabetic rats. Methods and Results: Triglyceride and Low-Density Lipoprotein (LDL)-cholesterol levels decreased in the STZ-induced diabetic groups treated with S. officinalis extract (SOE) compared to the corresponding levels in the control groups. Moreover, in the ALLinduced diabetic groups, SOE reduced triglyceride, LDL-cholesterol, and High-Density Lipoprotein (HDL)-cholesterol levels. Malondialdehyde (MDA) levels decreased significantly in the STZ and ALL-induced groups treated with SOE compared to the corresponding levels in the control group. Further, Glutathione (GSH) levels increased but did not reach statistical significance. The levels of Superoxide Dismutase (SOD) and Glutathione-S-Transferase (GST) showed a tendency to recover with SOE treatment in the STZ and ALL-induced diabetic groups. In addition, Catalase (CAT) levels in the SOE treatment group decreased significantly compared to those in the control group. Conclusions: These results suggest that SOE might be an effective agent in attenuating oxidative stress in diabetic patients by improving blood lipid profiles and inducing the anti-oxidative enzyme systems.