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- 저자 : 조종래(Chongrae Cho) (검색결과 3 건)
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Vitek Ⅱ에 의한 균동정 및 항균제감수성 결과에 균주보관시간이 미치는 영향
조종래,엄태현 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3
Objective : It would be frequent that routine tests in clinical microbiology laboratory are interrupted more than 2 days because of the five-day workweek legislation, which is thought to be launched near future in Korea. We investigated the impacts of the extended storage of bacterial colonies in secondary culture media on the bacterial identification and antibiotic susceptibility tests. Methods and Materials : Five strains of Staphylococcus aureus, 4 coagulase negative staohylococcus(CNS), 3 Escherichia coli. 4 Pseudomonas aeruginosa, and 4 Acineto-bacter baumannii, which are frequently isolated from clinical samples in Ilsan Paik Hospital were used for this study. We did the identification and antibiotic susceptibility tests by VITEK 2 system, using colonies stored for 2 days, 4 days, and 6 days in room temperature. Fesults : There were no changes in identification until 6 day storage. The percentages of the organism-antibiotic combinations in which minimum inhibitory concentration(MIC) changed more than 1 dilution were as follows; S. aureus 9.0%, CNS 17.5%, E. coli 10.0%, P. aeruginosa 23.7%, and A. baumannii 22.4%. The percentages with more than 2 dilution changes, which are considered to be significant, were as follows: S. aureus 0%, CNS 3.8%, 2. coli 1.7%. P. aeruginosa 5.3%, and A. baumannii 6.6%. The very major errors observed in S. aureus, CNS, E. coli. P. aeruginosa and A. baumannnii were 0%, 1.3%, 1.7%, 0% and 2.6%, respectively; for minor errors, 1.0%, 0%, 1.7%, 6.6% and 6.6%, respectively. Major errors were not observed in any of the organism-antibiotic combinations studied. Conclusion : We thought that it would be acceptable for clinical microbiology laboratory to delay the antibiotic susceptibility tests during holidays.
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엄태현,조종래 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3
Objective : ADVIA?? 1650(Bayer Inc., NY, USA) is an automated chemistry analyzer introduced into Korea in 1999. We evaluated the performance of ADVIA 1650 for precision, linearity, recovery, and compared it with CX7 autoanalyzer(Bookman Instruments, Inc., CA. USA). Methods and Materials : We used pool serum and commercial qualify control materials, such as Control 1 and Control 2(Bayer Inc., NY, USA). A total of 22 test items, such as glucose, blood urea nitrogen(BUN), creatinine, uric acid, total protein, albumin, aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phsphatase(ALP), total bilirubin, choesterol, triglyceride(TG), phosphorus(P), magnesium(Mg), creatine kinase(CK), lactate dehydrogenase(LD). ?-glutamyl transferase(GGT), amylase, sodium(Na), potassium(K), and chloride(Cl) were evaluated. Results : Within-run coefficients of variations(CVs) of all test items except total bilirubin were less than 5%. Between-day CVs were less than 5% except creatinine, AST, ALT, total bilirubin. All test items showed good linearity with CVs higher than 0.99. Recovery rates were in the range of 90% to 106%. The correlations with CX7 were good with CVs higher than 0.97 in most of the test items, except creatinine, total protein and sodium, which showed CVs lower than 0.90. Conclusion : We think that ADVIA 1650 is a useful automatic chemistry anaylzer for high-volume laboratories.
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