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RISS 인기검색어
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- 저자 : 조영롱 ( Young Long Cho ) (검색결과 3 건)
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Kaempferol 및 Kaempferol Rhamnosides의 항산화 활성 및 항염 효과에 관한 연구
이근하 ( Keun Ha Lee ),조영롱 ( Young Long Cho ),주철규 ( Chul Gue Joo ),주연정 ( Yeon Jeong Joo ),권순상 ( Sun Sang Kwon ),안수미 ( Soo Mi Ahn ),오수진 ( Su Jin Oh ),노호식 ( Ho Sik Rho ),박청 ( Chung Park ) 대한화장품학회 2011 대한화장품학회지 Vol.37 No.3
본 연구에서는 kaempferol 및 그 배당체의 항산화 및 항염증 효능을 평가하기 위해 free radical 소거활성, ROS inhibition assay, TARC 생성 억제 관련 실험을 수행하였다. 또한 kaempferol과 그들의 rhamnosides (α-rhamnoisorobin, afzelin, kaempferitrin)의 구조에 따른 생리적 활성의 상관관계를 조사하였다. Kaempferol과 α-rhamnoisorobin은 free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성(FSC50)에서 각각 62.5, 50.0 μM이 측정되었으며 afzelin과 kaempferitrin은 농도와 상관없이 free radical 소거활성을 나타내지 않았다. ROS inhibition assay에서는 시료 농도를 10, 50, 100 μM을 처리한 결과 kaempferol은 대조군(100 %)에 비하여 ROS 발현 농도를 각각 97.5, 57.8, 47.8 %로 감소하였으며 α-rhamnoisorobin은 93.1, 59.1, 41.4 %의 감소를 나타내었다. TARC (thymus and activation regualted chemokine) 생성 억제능 실험을 통해 시료의 항염증 효능을 평가한 결과 kaempferol은 10, 50, 100 μM 농도에서 대조군(100 %)에 대비하여 각각 48.8, 5.5, 4.4 %로 농도 의존적으로 TARC 생성을 감소시켰으며, α-rhamnoisorobin은 88.1, 19.0, 1.0 %로 TARC 생성의 감소 효능을 나타내었다. 결론적으로 kaempferol과 그들의 rhamnoside 중 kaempferol과 α-rhamnoisorobin은 항산화, 항염증에 대한 우수한 효능을 나타내는 것으로 사료되며 더 나아가서는 항노화 및 항염증에 효과가 있는 기능성 화장품에 응용 가능성이 있음을 시사한다. In this study, to evaluate the antioxidative activities and anti-inflammatory effects of kaempferol and its rhamnosides, we performed the free radical scavenging assay, ROS inhibition assay and TARC (thymus and activation- regulated chemokine) assay. Also, we studied physiological activity of kaempferol and its rhamnosides (α-rhamnoisorobin, afzelin, kaempferitn) by structure-activity relations. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities were determined with kaempferol (62.5 μM) and α-rhamnoisorobin (50.0 μM) but afzelin and kaempferitrin did not show free radical scavenging activities. Kaempferol showed a 97.5, 57.8, 47.8 % inhibition of ROS (reactive oxygen species) generated at concentrations of 10, 50 and 100 μM, compared to control (100 %). α-rhamnoisorobin showed a 93.1, 59.1 and 41.4 % inhibition of ROS at the same concentration. We investigated the inhibitory effects of kaempferol and its rhamnosides on TARC expression. Kaempferol showed a 48.8, 5.5 and 4.4 % inhibition of TARC generated at 10, 50 and 100 μM, compared to control. α-Rhamnoisorobin showed a 88.1, 19.0 and 1.0 % inhibition of TARC generated at the same concentration. In conclusion, these results indicate that kaempferol and α-rhamnoisorobin have good antioxidative activities and anti-inflammatory effects that could be applicable to new functional cosmetics for anti-aging and anti-inflammation.
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흑두 추출물이 모발 주기 anagen 시기의 증식과 분화에 미치는 영향
조영롱,이은정,조경혜 서울여자대학교 2010 자연과학연구논문집 Vol.22 No.-
A hair follicle is one of complex miniorgans in human body which has a life cycle of anagen, catagen and telogen. The three stages occur periodically and it is reported that this cycle is controlled by the balance of Bmp and noggin. This study attempts to verify in a molecular level if a Glycine Semen nigra extract known as a hair restorer has positive effect on maintaining autonomous anagen stage of DP cells isolated from 1 day-old rat back skin by expressing Bmp and noggin. It was proven that the Glycine Semen nigra extract stimulates mRNA expression of bmp4, bmp6 and their receptor bmpr1a, which maintains anagen stage and stimulates differentiation. The effect of Glycine Semen nigra extract on stimulating differentiation of DP cells was also verified as the extract increases the expression of lef1 and other differentiation marker genes (wif1, akp2, alx4) for differentiation and the enzyme activity of alkaline phosphatase. It was observed that Glycine Semen nigra extract induces anagen phase and increases the gene expression of Bmp antagonist noggin which stimulates proliferation of DP cells. The expression of various growth factors (fos, vegf, fgf2) related to the proliferation of DP cell and hair follicle increases. The expression of wnt7a, which maintains anagen and stimulates cell multiplication, and its effector β-catenin also increased. Therefore, it was verified that Glycine Semen nigra extract has positive effect on hair growth by maintaining anagen stage, at which multiplication and differentiation of follicular cells actively occur. Glycine Semen nigra extract is expected to be used as a hair tonic or a hair growth promoter that maintains anagen stage and healthy hair.
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Minoxidil에 의한 모발절대휴지기 탈출 유도 효과
이은정,조영롱,조경혜 서울여자대학교 자연과학연구소 2011 자연과학연구논문집 Vol.23 No.-
Hair follicle (HF), a small physiological organ in the human body which produces hair, repeats a four-phased hair cycle consisting of anagen, catagen, telogen, and exogen phases and affects the growth, maintenance, and loss of hair. Recent studies revealed that the hair growth and loss are controlled by the balance between bmp and nog expressed from dermal papilla cells (DP cells) which control the physiological characteristics of the hair growth cycle and that a phase transition from refractory telogen phase to competent telogen phase, caused from the reduction in expression of bmp gene in the telogen phase, is indispensible for the regeneration of hair cycle. This study is conducted to verify the effect of minoxidil, a depilatory lotion widely used around the globe on the molecular mechanism in the hair cycle process occurring in the DP cells, especially in the expression of bmp and nog genes. DP-enriched cells were separated and cultured from the back skin of 1 day-old new born rats and observed the effects after processing minoxidil on the cells. Minoxidil reduced the expression of bmp4 and bmp6 genes, which stimulate differentiation but inhibit multiplication of DP cells in the hair cycle control process, and reduced the expression of bmprla gene, as its receptor. Minoxidil also reduced the expression of nog genes known as an antagonist of bmp produced from DP cells and an essential element in the telogen-anagen phase transition. From this results, minoxidil induced the DP cells to stay at the competent telogen phase, a prior phase of the initiation of the anagen phase, and to escape from refractory telogen phase of the hair cycle. Also, minoxidil induced DP cells to an ‘undifferentiated state of hair cell' of competent telogen phase, a prior phase of early anagen phase, rather than that inducing the cell to an initiation phase of hair cycle, the anagen phase. Minoxidil reduced the expression of nog gene which is an essential element in the regeneration of hair cycle, but increased the expression of bfgf gene, a upstream growth factor which stimulates the expression of nog and vegf genes which foster the growth of DP cell. Thus, it can be suggested that there is possibility that minoxidil indirectly affects telogen-anagen phase transition and the initiation of anagen phase. In conclusion, this study verified that minoxidil leads escape from refractory telogen phase of a hair growth cycle and thereby reduces the period of the telogen phase. Key words:dermal papilla cells, minoxidil, bmp, noggin, hair cycle.
ScienceON
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