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Osmotic Shock에 의한 Vibrio vulnificus사멸에 관한 연구
정선식,이준행,조순흠 대한미생물학회 1987 大韓微生物學會誌 Vol.22 No.2
As a proeess to establish an effective preventive measure of V. vulnificus septicemia, bactericidal effect of distilled water against V. vrclnificus was studied. When about 2,0X10^7 CFU/ml of V. vulnificus was inoculated in distilled water, a dramatic deerease in the numbee of viable bacteria by 5 to 6LOG was observed in 5 minutes. Bactericidal kinetic curves could be divided into the first rapid killingphase until 1 minute and the later slow killing phase after then, showing the heterogeneity of the bacterial population inoculated. When V. vnlngcgs was inoculated in saline solutions having various salinities, signi6cant decrease in the number of viable bactcria was noted only at salinities under 0.2%. The hig- her was the concentration of NaC1, the greater was the degree of protection against osmotic shock. When glucose, NaC1₂, MgCland CaCl₂, were diluted with deionized water to give same osmolarities and V. vuleificus was inoculated in esch of them to compare the bactericidal curves plotted during the first 5 minutes after inoculation, the protection efficiencies were in the order of MgC1₂$gt; CaCl₂$gt; NaC1₂$gt;glucose. Above results indicate that treatment(or thorough washing) of contaminated sea animals or other products with distilled water can be used as a preventive measure of V. vulnifzcus septicemia, and divalent cations can protect V. vulnifcus to osmotic shock with high efficiency.