실버메이트 로봇을 위한 행위표현에 관한연구

조수정(Su-Jeong Cho),최경현(Kyung-Hyun Choi),김병국(Byung-Kook Kim) 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.6

In order to execute the various and complex tasks for a intelligent robot, more improvement not only in mechanical system architecture but also in control system architecture is needed. The suggested control architecture consists of three layers of deliberative, sequencing, and reactive as hybrid control architecture. This paper addresses a control architecture of robots, and a behavior representation methodology. Multi-layer behavior model is employed to represent the desired task. Finally, the suggested hybrid control architecture and methodology of behavior representation are verified to execute a given task successfully by employed UML logic simulation.

경근이완요법이 뇌졸중 환자의 주관절 경직과 상지기능에 미치는 효과

김정화 ( Jeong Hwa Kim ),최선임 ( Sun Im Choi ),허정자 ( Jung Ja Her ),조수정 ( Su Jung Cho ) 경희대학교 동서간호학연구소 2003 동서간호학연구지 Vol.8 No.1

Purpose: The Purpose of this study was to test the effects of the Muscles Along Meridians Release Therapy on the function of upper limb as a means of nursing intervention. Method: The design used for this study was quasi-experimental with a nonequivalent control group pretest-post test design. The subjects were 40 stroke patients who were admitted in K oriental medical center of K University. This study was carried out from 6, May to 18, October, 2003. The experimental group (21) and the control group (19) were assigned by means of Participation order. The experimental group took Muscles Along Meridians Release Therapy on affected upper limb for 3 minutes daily for 2 weeks. Outcome were assessed by Modified Ashworth Scale, VAS, Fugl-Meyer score and goniometer. Data were analysed by SPSS PC. Result: After 2 weeks of treatment, function of affected upper limb, elbow joint spasticity were significantly better than control group, but, there was no significant difference in pain between experimental group and control group. Conclusion: The above results state that the Muscles Along Meridians Released Therapy could be an effective intervention for improving upper limb function and elbow joint spasticity of stroke patients.

부위별 흰색 느티만가닥버섯 추출물의 항산화 활성

김수철(Su Cheol Kim),권현숙(Hyun Sook Kwon),김철환(Chul Hwan Kim),김혜수(Hye Soo Kim),이창윤(Chang Yun Lee),조수정(Soo Jeong Cho) 한국생명과학회 2016 생명과학회지 Vol.26 No.8

본 연구에서는 천연물 유래 항산화 소재로서 흰색 느티만가닥버섯(Hypsizygus marmoreus)의 부위별 이용 가능성을 알아보기 위하여 흰색 느티만가닥버섯을 갓(pileus)과 대(stipe)로 분리하여 열수와 80% 에탄올에 침지한 다음 부위별 항산화 활성을 조사하였다. 흰색 느티만가닥버섯은 에탄올 추출물(갓 부위 923.47±0.18 mg GAE/100g, 대 부위 324.05±0.03 mg GAE/100 g)에 비해 열수 추출물(갓 부위 1137.39±0.38 mg GAE/100 g, 대 부위 700.86±0.06 mg GAE/100 g)의 총 폴리페놀 함량이 높게 나타났고 대 부위보다는 갓 부위의 총 폴리페놀 함량이 높게 나타났다. DPPH 라디칼 소거 활성은 10 mg/ml의 농도에서 에탄올 추출물 갓 부위의 활성이 47.32±0.23%로 가장 높았으며 ABTS 라디칼 소거 활성도 10 mg/ml의 농도에서 에탄올 추출물 갓 부위의 활성이 57.33±0.10%로 가장 높았다. 환원력과 ORAC 지수는 에탄올 추출물에 비해 열수 추출물이 높게 나타났고 환원력은 갓 부위와 대 부위에 큰 차이가 없었으나 ORAC 지수는 대 부위보다는 갓 부위가 높게 나타났다. 흰색 느티만가닥버섯 추출물의 세포 독성은 WST-1 assay를 이용하여 추출물이 RAW 264.7 세포에 미치는 영향으로 평가하였다. 이상의 결과를 종합해보면, 흰색 느티만가닥버섯의 총 폴리페놀 함량과 항산화 활성은 추출물의 농도가 증가함에 따라 증가하는 경향을 나타내었으며 대 부위보다는 갓 부위의 항산화 활성이 우수하였다. Hypsizygus marmoreus (white cultivar), also called white beech mushrooms, are edible mushrooms commercially cultivated in Korea and Japan. This study was carried out to evaluate the antioxidant properties of H. marmoreus. H. marmoreus fruit bodies were divided into pileus and stipe. The pileus and stipe were extracted into water and 80% ethanol and their antioxidant activities were analyzed. The total polyphenol content was highest in the water extract (pileus 1137.39±0.38 mg of GAE (gallic acid equivalents)/100 g, stipe 700.86±0.06 mg of GAE/100 g) compared to the ethanol extract (pileus 923.47±0.18 mg of GAE/ 100 g, stipe 324.05±0.03 mg of GAE/100 g). Ethanol extracts from pileus showed better scavenging ability on DPPH (47.32±0.23% at 10 mg/ml) and ABTS (57.33±0.10% at 10 mg/ml) than the stipe and water extract groups. Water extract from pileus were more effective in reducing power and ORAC (oxygen radical absorbance capacity) value than stipe and ethanol extract. The toxicity of water and ethanol extracts was investigated using WST-1 (Water Soluble Tetrazolium salt) assay on the mouse macrophage cell line RAW 264.7. Overall, total polyphenol content and antioxidant activities of extracts from H. marmoreus increased in a dose dependent manner while pileus was showed better total polyphenol content and antioxidant activities than stipe.

Silymarin과 작약감초탕 병용투여의 C57BL/6 마우스 간조직 지질축적 및 염증 억제효과

최정원,조수정,신미래,박해진,Choi, Jeong Won,Cho, Su-Jung,Shin, Mi-rae,Park, Hae-Jin 대한본초학회 2022 大韓本草學會誌 Vol.37 No.5

Objective : The aim of the present study is to examine hepatic lipid-lowering and anti-inflammatory effects of silymarin combined with Jakyakgamcho-tang on non-alcoholic fatty liver disease in a high fat diet-induced obese mice model. Methods : C57BL/6 mice were divided into four dietary groups: (1) Normal, (2) Control (60% high-fat diet), (3) Control + silymarin 50 mg/kg/day (Silymarin), (4) Control + Silymarin 50 mg/kg/day + Jakyakgamcho-tang 100 mg/kg/day (SPG). After 12 weeks administration, mice were sacrificed and lipids and inflammation-related biomarkers were analyzed liver and plasma. Results : Silymarin and SPG treatments significantly lowered body and liver weights compared to the Control. Serumlipids (triglyceride (TG), total cholesterol) and pro-inflammatory cytokines (tumor necrosis factor alpha, interleukin 1𝛽, and IL-6) concentrations were significantly lowered in the Silymarin and SPG groups than the Control group. Silymarin and SPG treatments suppressed hepatic TG level and hepatic lipid droplets compared to the Control. Theses two treatments significantly increased hepatic kinase B1 and AMP-activated protein kinase protein levels, and significantly decreased hepatic key lipogenic enzymes (acetyl-CoA carboxylase, fatty acid synthase and stearyl coenzyme A desaturase 1) protein levels than the Control. SPG also significantly increased hepatic fatty acid oxidation-related protein (peroxisome proliferator-activated receptor alpha and uncoupling protein 2) levels than the Control. Conclusions: Silymarin and SPG suppressed hepatic lipid accumulation by regulating hepatic protein expression, and lowered blood pro-inflammatory cytokines concentrations though the synergic effect of silymarin and Jakyakgamchotang was not clear.

베타글루칸 함량이 높은 큰느타리버섯 선발을 위한 SCAR marker 개발

김수철,김혜수,조용운,류재산,조수정,Kim, Su Cheol,Kim, Hye Soo,Cho, Yong Un,Ryu, Jae-San,Cho, Soo Jeong 한국버섯학회 2015 한국버섯학회지 Vol.13 No.1

본 연구는 큰느타리버섯의 베타글루칸 고함유 형질에 관련된 SCAR marker를 개발하기 위해 수행되었다. operon사의 OPA(20개), OPB(20개), OPL(20개), OPP(20개), OPR(20개), OPS(20개) 등 총 120개 primer를 random primer(10 mer)로 사용하여 대립 계통 9종과 베타글루칸 고함유 계통 9 종을 대상으로 RAPD를 이용한 bulked segregant analysis를 실시하여 OP-R03 primer로부터 대립 계통에는 나타나지 않고 베타글루칸 고함유 계통에만 나타나는 특이적인 RAPD 밴드를 얻었다. OP-R03 primer를 이용한 RAPD 결과, 약 91 bp 부근에서 베타글루칸 고함유 계통에 특이적인 DNA 밴드가 관찰되었으며 이 DNA 밴드의 염기서열 말단을 근거로 SCAR 마커로 사용할 specific primer인 OP-R03-1-F와 OP-R03-1-R를 디자인하였다. SCAR 마커 OP-R03-1-F/-1-R primer를 이용하여 PCR을 수행한 결과에서도 91 bp 부근에서 대립 계통과 구별되는 DNA 밴드가 베타글루칸 고함유 계통에서 확인되었으며 random primer인 OP-R03 primer를 이용하여 PCR을 수행했을 때보다 재현성이 높고 진한 DNA 밴드임을 확인할 수 있었다. In this study, SCAR marker that differentiates Pleurotus eryngii strains with higher ${\beta}$-glucan from control strain was developed. Genomic DNAs of 9 control strains of Pleurotus eryngii and 9 Pleurotus eryngii strains with higher ${\beta}$-glucan were analyzed by bulked segregant analysis (BSA) using randomly amplified polymorphic DNA (RAPD). One-hundred twenty RAPD primers were screened on bulked DNA samples and a unique DNA fragment with the size of 91 bp was yielded by OP-R03 primer from the Pleurotus eryngii strains with higher ${\beta}$-glucan. A sequence characterized amplified region (SCAR) marker, designated as OP-R03-1-F and OP-R03-1-R, was designed on the basis of the determined sequence. The PCR analysis with the OP-R03-1 primer showed that this SCAR marker can clearly distinguish the Pleurotus eryngii strains with higher ${\beta}$-glucan from the control strains.

Cloning and Identification of Essential Residues for Thermostable β-glucosidase (BgIB) from Thermotoga maritima

홍수영,조계만,김용희,홍선주,조수정,조용운,김훈,윤한대,Hong, Su-Young,Cho, Kye-Man,Kim, Yong-Hee,Hong, Sun-Joo,Cho, Soo-Jeong,Cho, Yong-Un,Kim, Hoon,Yun, Han-Dae Korean Society of Life Science 2006 생명과학회지 Vol.16 No.7

A hyperthermophilic bacterium Thernotoga maritima produced thermostable ${\beta}-glucosidase$. The gene encoding ${\beta}-glucosidase$ from T. maritima MSB8 was cloned and expressed in Escherichia coli. The en-zyme (BgIB) hydrolyzed ${\beta}-glucosidase$ linkages between glucose and alkyl, aryl of saccharide groups such as salicin, arbutin, and $_pNPG$. The insert DNA contained ORF with 2,166 bp encodes a 721 amino acids (calculated molecular mass of 80,964 and pl of 4.93). The amino a.id sequence of BglB showed the similarity to family 3 glycosyl hydrolases. The molecular weight of the enzyme was estimated to be approximately 81kDa by MUG-nondenaturing PAGE (4-methylumbelliferyl 13-D-glucoside-nondenaturing polyacrylamide gel electophoresis) and SDS-PACE. The ${\beta}-glucosidase$ exhibited maximal activity at pH 7.0 and $80^{\circ}C$. By exchanging two possible residues (Glu-232 and Asp-242) to Ala by site-directed mutagenesis method, it was found that these were essential for enzymatic activity. 초고온성 세균인 Thermotoga maritima로부터 ${\beta}-glucosidase$ 유전자를 클로닝한 후 대장균 숙주에서 발현시켰다. 이 효소는 salicin, arbutin, $_pNPG$과 같은 탄소원의 ${\beta}$-글루코시드 결합을 가수분해하였다. 721개의 아미노산을 암호화하는 2,166 bp의 DNA 염기서열로된 유전자이였다. 다른 ${\beta}-glucosidase$ 효소들과 단백질 유사성을 비교한 결과 glycosyl hydrolase family 3에 속하였으며 MUG-nondenaturing PAGE와 SDS-PAGE에 의해 확인된 단백질의 크기는 약 81 kDa이었다. 효소활성은 pH 7.0, $80^{\circ}C$에서 가장 높은 활성을 나타냈으며 이 효소의 아미노산 서열에 있는 두 개의 아미노산 잔기 (232번 글루탐산과 242번 아스파르트산 잔기)를 알라닌으로 치환시켜 활성이 없어지는 것으로 보아 이 두 잔기가 효소활성에 중요한 역할을 하는 것으로 추정된다.

Tyrosinase Inhibitory Activities of Meso-dihydroguaiaretic Acid from Machilus thunbergii

Hyun Sook Kwon(권현숙),Kyung Dong Lee(이경동),Su Cheol Kim(김수철),Soo Jeong Cho(조수정) 한국생명과학회 2015 생명과학회지 Vol.25 No.11

후박나무(녹나무과)는 한국과 일본 등지에 서식하는 상록 교목으로 한국, 중국, 일본에서 부종, 복통, 복부 팽만 등의 질병 치료를 위해 오랫동안 사용되어오고 있다. 본 연구에서는 후박나무 껍질을 메탄올에 추출하고 메탄올 추출물을 헥산, 클로로포름, 부탄올에 순차적으로 분획하였다. 클로로포름 분획물로부터 2종의 화합물을 분리하였으며 분리된 화합물1과 2의 구조는 ¹H-, <SUP>13</SUP>C-NMR과 참고 문헌 데이터에 의해 dibenzylbutane lignin 화합물인 macelignan (1)과 meso-dihydroguaiaretic acid (2)로 동정되었다. 분리된 화합물들의 tyrosinase 저해 활성을 측정한 결과, 화합물 2는 tyrosinase 저해 활성 중 monophenolase (IC50 = 10.2 μM)에 대해 높은 저해활성을 나타내는 경쟁적 저해제였으며 효소에 결합하는 화합물 2의 저해 상수(Ki 값)는 4.8 μM였다. 따라서 meso-dihydroguaiaretic acid (2)는 멜라닌 생합성과 관련된 피부 질환 치료를 위한 잠재적 후보가 될 수 있을 것으로 판단된다. Machilus thunbergii (Lauraceae) is an evergreen tree cultivated in Korea and Japan. M. thunbergii has long been used as a traditional medicine in Korea, China, and Japan to treat various diseases, including edema, abdominal pain, and abdominal distension. In this study, dried stem bark of M. thunbergii extracted in methanol and extract was partitioned into n-hexane, CHCl₃, and BuOH. The CHCl₃-soluble extracts chromatographed on silica gel column using a CHCl₃/acetone and n-hexane/EtOAc mixture to afford Compound 1 and 2. Two dibenzylbutane lignans, macelignan (1) and meso-dihydroguaiaretic acid (2), were isolated from the CHCl₃-soluble extract of M. thunbergii stem bark. The structures of 1 and 2 were determined by <SUP>1</SUP>H- and <SUP>13</SUP>C-NMR spectroscopic data analyses and a comparison with literature data. The tyrosinase inhibitory activity of the isolated compounds was evaluated. Among these compounds, Compound 2 strongly inhibited the monophenolase (IC50=10.2 μM) activity of tyrosinase. A kinetic analysis showed that Compound 2 was a competitive inhibitor. The apparent inhibition constant (Ki) for Compound 2 binding to free enzyme was 4.8 μM. Based on these results, it can be concluded that meso-dihydroguaiaretic acid (2) is a potential candidate for the treatment of melanin biosynthesis-related skin diseases.

백출(白朮)의 항산화 효과가 DSS 유발 궤양성 대장염 모델에 미치는 영향

박석만 ( Seok Man Park ),이세희 ( Se Hui Lee ),정다운 ( Da Un Jeong ),조수정 ( Su-jung Cho ),신미래 ( Mi-rae Shin ),박해진 ( Hae-jin Park ),노성수 ( Seong-soo Roh ) 대한본초학회 2022 大韓本草學會誌 Vol.37 No.1

Objectives : Although the pharmacological effects of anti-inflammatory and antioxidant action of Atractylodes macrocephala Koidzumi water extract (AM) have been proven from many studies, reports on the antioxidant effect of AM on ulcerative colitis (UC) are scarce. Therefore, we aimed at evaluating the anti-oxidant effect of AM on the DSS-induced UC model. Methods : To induce ulcerative colitis, 8-week-old male Balb/c mice received 5% DSS in drinking water for 1 week. After 1 week of adaptation, mice were divided into four groups (n=8 each) for use as normal (Normal), DSS Control (Control), DSS + AM 100 ㎎/kg (AM100)-treatment, DSS + AM 200 ㎎/kg (AM200)-treatment. After 1 week of the experiment, the animals were sacrificed, and the extracted colon tissue was analyzed for protein through western blot. Results : As a result of confirming the macroscopic changes in colon tissues to confirm the therapeutic effects of AM, the decrease in colon length was suppressed in the AM treatment group compared to the control group. In addition, as a result of biochemical analysis, AM administration significantly reduced serum glutamic oxalacetic transaminase, glutamic pyruvate transaminase levels and tissue malondialdehyde levels. As a result of confirming the protein expression level through western blot, AM administration significantly decreased the expression of NADPH-related proteins such as NOX2, p22^phox, and iNOS, but significantly increased the expression of SOD, catalase, and GPx-1/2. Conclusions : AM may improve DSS-induced UC in mice by modulating NADPH and antioxidant-related proteins. In conclusion, AM showed an antioxidant effect through the improvement of oxidative stress on UC.

모유강화제(HMF)가 흰쥐의 성장과 영양상태에 미치는 영향

이미린(Mi-Rin Lee),박미나(Mi-Na Park),조수정(Su-Jung Cho),전정욱(Jeong-Wook Jeon),최유영(You-Young Choi),박정식(Jung-Sik Park),김완식(Wan-Sik Kim),이연숙(Yeon-Sook Lee) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.1

본 연구에서는 갓 이유한 3주령의 어린 흰쥐에게 일반조제유, 미숙아용조제유, 모유강화제(HMF)를 보충한 조제유를 급여하여, 분유의 종류에 따라 성장과 영양상태 및 생리기능에서 차이가 나타나는지 밝히고자 하였다. 특히, 일반조 제유에 HMF를 첨가하여 급여한 경우와 미숙아용조제유를 급여한 경우가 일반조제유 섭취와 비교하여 어떠한 차이가 있는지를 알아보았다. 실험식이는 매일유업주식회사 제품인 일반조제유(프리미엄명작-1; 분말), 미숙아용조제유(베이비웰 프리미; 분말), 모유강화제(베이비웰 에이치엠에프(HMF); 액상)를 사용하였다. 일반조제유군에게는 프리미엄명작-1에 증류수 13%를, 미숙아용조제유에게는 베이비웰프리미에 증류수 14%를, 그리고 조제유+HMF군에게는 프리미엄명작-1에 HMF를 14% 첨가하여 반고형식으로 실험식이를 만들어 3주 동안 급여하였다. 체중증가량 및 식이섭취량은 미숙아용조제유군과 HMF보충군이 일반조제유군에 비하여 유의적으로 증가하였다(p<0.05). 간, 신장, 비장의 체중에 대한 장기 중량 비는 군간 차이가 없었으나, 신장주변지방과 부고환지방은 미숙아용조제유군과 HMF보충군이 일반조제유군에 비하여 유의적으로 증가하였다(p< 0.05). 혈청의 총 지질과 총 콜레스테롤, HDL-콜레스테롤은 실험군간 유의적인 차이가 없었다. 반면 혈청의 중성지방은 미숙아용조제유군이 식이 중 지방함량의 영향을 받아 다른 군에 비해 유의적으로 높았다(p<0.05). 간 조직의 총 지질은 식이에 따른 차이는 없었으나, 혈청 중성지방과 총 콜레스테롤은 미숙아용조제유군과 HMF보충군이 일반조제유군에 비해 유의적으로 높았다(p<0.05). 분 중 총 지질과 중성지방배설량은 미숙아용조제유군이 나머지 두 군에 비해 유의적으로 증가하였다(p<0.05). 분 중 총 콜레스테롤 배설량은 미숙아용조제유가 유의적으로 가장 높았고, 일반조제유군이 HMF군보다 유의적으로 증가하였다(p<0.05). 혈청 생화학 지표에서는 albumin과 total protein이 미숙아용조제유군에서 유의적으로 증가하였다(p<0.05). GOT는 군간 유의적 차이를 보이지 않았지만 GPT는 HMF보충군에서 증가하였다. BUN은 일반조제유군이 유의적으로 높게 나타났고, 혈중 칼슘 농도는 미숙아용조제유군이 다른 군에 비해 유의적으로 높았다(p<0.05). 혈액의 헤모글로빈 농도는 미숙아용조제유군과 HMF보충군이 일반조제유군에 비하여 유의적으로 높게 나타났다(p<0.05). 혈청의 성장호르몬 농도는 미숙아용 조제유군이 일반조제유군과 HMF보충군에 비하여 유의적으로 증가하였다(p<0.05). This study was conducted to evaluate the efficacy of human milk fortifier (HMF) on growth and nutritional status in growing rats fed infant formula supplemented with HMF. Three week-old male Sprague-Dawley rats were divided into three groups and fed regular formula (RF), premature formula (PF) and regular formula fortified with HMF (RF+HMF) diets for 3 weeks. There was no significant difference in weight gain among groups. However, a significant increase of food intake was observed in PF and RF+HMF groups compared with RF group. With increasing food intake, the intakes of carbohydrate and protein were significantly increased in PF and RF+HMF groups. The weight of perirenal fat was significantly increased in rats fed RF+HMF; however, the weights of liver, kidney and spleen were not significantly different among groups. Although total lipids, total cholesterol, HDL-cholesterol concentrations of serum were not significantly different among groups, triglyceride was significantly increased in PF group. The triglyceride and total-cholesterol of liver were significantly increased in rats fed regular formula fortified with HMF and PF compared with RF group. Glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), creatinine (Cre) and blood urea nitrogen (BUN) in serum showed no significant difference among groups. The concentration of growth hormone was significantly increased in PF group compared with other groups. The concentration of hemoglobin was significantly increased in rats fed PF and RF+HMF. These results suggest that the supplementation of human milk fortifier in growing rats may promote growth as increasing food intake and lipid contents in tissues and prevent the anemia of infants.

다문화 학생의 모국어 사용이 학생 및 부모요인에 미치는 영향

임동선(Dongsun Yim),김지애(Ji Ae Kim),오수진(Soo Jin Oh),이은진(Eun Jin Lee),조수정(Su Jeong Cho),박원정(Won Jung Park) 한국언어치료학회 2021 言語治療硏究 Vol.30 No.1