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파라핀 포매조직의 DNA 분석 : Proteinase K를 사용하는 새로운 절차
김도희,김낙준,조무자,김동란 대한임상검사과학회 1997 대한임상검사과학회지(KJCLS) Vol.29 No.1
DAN content analysis of formalin fixed paraffin-embedded tissue permits determination of the influence of DNA content on the prognosis in cancer patients. Of key importance in such analysis is the accuracy of DNA content determination. Digestion with enzymes playsand important role in obtaining good results in cellular DNA content measurements from paraffin-embedded tissue using flow cytometer. We have compared three groups of DNA histograms obtained from fresh tumor tissue, paraffin embedded same tumor tissue digestedpepsin alone(Hedley method) and proteinase K digestion with each other. Concerning the coefficient of variation(CV), the fresh tissues produced the lowest CV and that of proteinase K method was lower than that of the pepsin digestion alone method. However,the proteinase K digestion method had important drawbacks such as loosing aneuploid fraction due to overdigestion. The mean CV of the pepsin digestion alone method was 4.92. Although the CV s of the pepsin digestion method using paraffin embedded tissue were a little higher than that of fresh tissue, the paraffin blocks could be the best samples to analyse DNA content of tumors, because we can use the tumor tissue with minimum contamination of normal tissue. .
급성 골수성 백혈병에서 Adenosine deaminase 측정의 임상적 유용성
고광상,박정오,강성훈,김재석,박순철,서연실,김기헌,조무자 임상병리검사과학회 1999 임상병리검사과학회지 Vol.31 No.2
배경 : Adenosine deaminase(ADA) 는 adeno-sine을 inosine으로 deamination하는 반응을 촉매하는 효소로, 혈액 종양을 비롯한 결핵, 간 질환, 등의 다양한 질환에서 활성도가 증가하는 것으로 알려져 있으며, 여러 질환에서 연구가 이루어지고 있으나 측정의 기술적 어려움으로 이용이 확대되지 못하였다. 그러나 최근 고안된 자동 화학 분석기를 이용한 ADA 와 동위효소의 분별정량 방법으로 ADA 측정의 임상적 적용이 용이 해졌다. 저자들은 이 방법으로 혈장을 이용하여 AML에서의 ADA활성도와 동위효소의 분포양상의 특성과, 치료경과에 따른 변화를 관찰하여 AML에서 ADA측정의 임상적 유용성을 알아보고자 하였다. 방법 : AML 환자군 16 예와 정상 대조군 25 예의 혈장 ADA와 동위효소의 활성도를 측정 비교하고, 환자군중 10 예에서는 치료 후 1주 간격으로 최고 8 주까지 추적 관찰을 시행하였다. ADA측정은 자동화학 분석기로 발색 정량하고, 동위효소의 측정은 ADA_(1) 선택적 억제제인 EHNA를 첨가하여 ADA_(2)를 측정하는 방법을 이용하였다. 결과 : AML 환자 군 16명의 혈장 ADA 활성도는 34.0±20.8IU/L로 정상 대조군 25명의 혈장 ADA 활성도 14.28±3.26IU/L에 비해 유의하게 높았다. 정상 대조군에서는 ADA2 가 75.0%로 우세한 것에 비해 AML군에서는 ADA_(1)이 62.5%로 우세하였고, ADA_(1)은 모두 정상대조군보다 높은 값을 보였다 . AML 환자 군 중 추적 검사를 시행하였던 10 예 (완전관해 5예 , 불완전관해 5예)에서 ADA 값과 ADA1이 10예 모두에서 4주 안에(평균 2.8주) 정상범위로 감소하였고, 5 예에서 ADA활성도가 정상범위로 감소한 후 1 주 후까지 ADA_(1)활성도가 정상범위 이상으로 지속되었다. 결론 : AML의 진단과 경과 추적에 혈장에서 측정한 ADA활성도와 동위효소 분포 양상을 이용할 수 있으며, 특히 ADA_(1)이 total ADA나 ADA_(2)보다 더 유용함을 알 수 있었다. ADA 활성도는 치료 후 관해여부와 관계없이 세포수가 감소함에 따라 모두 감소하므로 관해 여부를 예측 할 수는 없으나, 재발을 검출할 목적으로 이용할 수 있으며, 이때는 ADA 활성도가 모두 정상범위로 감소하는 치료 후 4 주 이후부터 검사하는 것이 효율적이라고 생각된다. Adenosine deaminase(ADA) activiteis in sera are increased in various disease such as hematologic malignancies, tuberculosis, and liver diseases. To evaluate the clinical value of measuring ADA and its isoenzyme activites in plasma with the automated assay in acute myeloid leukemia, we compared ADA and its isoenzyme activities in normal control group with those in acute myeloid leukemia group, and followed up those during chemotherapy in AML group. The measurements of plasma ADA and its enzymes, especially ADA_(1) were useful for AML diagnosis. But they were not valuable markers to discriminate between complete remission and incomplete remission. For the detection of relapse, it could be valuable to measure ADA activities beyond 4 weeks after chemotherapy.
비예기 항체 선별 검사의 예민도에 영향을 미치는 인자 연구
이규형 ( G. H. Lee ),계효인 ( H. I. Gye ),유소영 ( S. Y. You ),정찬호 ( C. H. Chung ),조무자 ( M. J. Cho ),김동언 ( D. E. Kim ) 대한임상검사과학회 1998 대한임상검사과학회지(KJCLS) Vol.30 No.2
Unexpected antibody Screening is essential for transfusion practice to prevent hemolytic transfusion reaction. So it is very important to maintain high sensitivity in unexpected antibody screening test. We analyzed the detection limits of anti - D antibody in various test and sample condition to estimate the influence of factors on the sensitivity ofunexpected antibody screening test. Regardless of washing procedure, the diluted anti - D antibody with normal saline were detected with same sensitivity by microplate method. In the detection of the diluted anti-D antibody in serum by microplate method, omittion of washing procedure made the sensitivity low. With column agglutination method, the detection sensitivity of the diluted anti-D antibody in serum was higher than that in saline. In conc1usion, the neutralization of anti - human globulin have major influence on the sensitivity of antibody detection by microplate method. But the factor having major influence on the sensitivity of column agglutination method is the other one than the neutralization of anti-human globulin.
급성 골수성 백혈병에서 Adenosine deaminase 측정의 임상적 유용성
고광상 ( Kwang Sang Kho ),박정오 ( Chung Oh Park ),강성훈 ( Seung Hun Kang ),김재석 ( Jae Suk Kim ),박순철 ( Seun Cherl Park ),서연실 ( Yeon Sil Seo ),김기헌 ( Ki Heun Kim ),조무자 ( Mu Ja Joe ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
Adenosine deaminase(ADA) activiteis in sera are increased in various disease such as hematologic ma1ignancies, tuberculosis, and liver diseases. To eva1uate the c1inica1 va1ue of measuring ADA and its isoenzyme activites in plasma with the automated assay in acute myeloid leukemia, we compared ADA and its isoenzyme activities in norma1 control group with those in acute myeloid leukemia group, and followed up those during chemotherapy in AML group. The measurements of plasma ADA and its isoenzymes, especia11y ADAl were useful for AML diagnosis. But they were not va1uable markers to discriminate between complete remission and incomplete remission. For the detection of relapse, it could be va1uable to measure ADA activities beyond 4 weeks after chemotherapy.
최혜순 ( Hye Soon Choi ),오안수 ( An Soo Oh ),홍재화 ( Jae Hwa Hong ),강승희 ( Seung Hee Kang ),김진순 ( Jin Soon Kim ),이영채 ( Young Chae Lee ),정진화 ( Jin Hwa Jung ),김기헌 ( Ki Heun Kim ),조무자 ( Moo Ja Cho ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
The Pelger-Huet anomaly is a benign anomaly of leukocytes that it is characterized by reduced number of nuclear segments and is inherited as autosomal donimant trait. The nuclei appear fod-like, dumbbell-shaped, peanut-shaped or spectacle-like with round or oval individual lobes but the Pelger-Huet cells are functionally normal. A 11-year-old boy was admitted for chemotheraphy of acute lymphoblastic leukemia. The peripheral blood smears reveal that mahority of neutrophils have one two lobes per mucleus, His father``s peripheral blood showed the same findings, We report a family case of Pelger-Huet anomaly.