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인면역결핍 바이러스 pol 유전자 염기서열 결정에 의한 지도부딘 ( ZDV ) 내성 돌연변이의 탐지
조영걸(Young Geol Jo),이희정(Hee Jung Lee),성흥섭(Heung Suk Sung),김유겸(Yoo Gyum Kim),김영봉(Young Bong Kim),이용진(Yong Jin Lee),김미정(Mi Jung Kim),김대곤(Dae Gon Kim),원영호(Young Ho Won),조군제(Goon Je Jo) 대한바이러스학회 1999 Journal of Bacteriology and Virology Vol.29 No.4
The nested polymerase chain reaction (PCR) assay was used to determine the sequences of reverse transcriptase (RT) codons 41, 67, 70, 210, 215 and 219 of human immunodeficiency virus-1 (HIV-1) pol gene. Template DNA was obtained from uncultured peripheral blood mononuclear cells from 27 Korean HIV-1 infected patients treated with ZDV and Korean red ginseng. The second PCRs were done for 2 separated regions (RT codons 13-98 and 152 259) with 5 ㎕ of the first PCR product. Nucleotide sequences were determined by direct sequencing. In the 27 patients, CD4+ cell count decreased from 230±117/㎕ to 152±162/㎕ for 46±26 months (Mo), and actual duration of ZDV intake was 27±16 Mo. In the 16 patients who had been treated with ZDV therapy ≥25 Mo, the incidences of 70R, 215F/Y, and 41L were 61%, 28% and 22%, respectively and those of 67N, 210W and 219Q were 17%. The incidences of 215F/Y were 6.7% for group ≤12 Mo treatment, 22.7% for group with 13 to 24 Mo, and 27.8% for group ≥25 Mo. There was no mutation in 9 patients. It might be associated with the interruption of ZDV therapy for more than 6 months in 6 patients. This study shows that the detection of mutation could be useful prognostic marker with other clinical and virological data, and very low mutation rate is dectected compared to overseas reports.