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메치오닌 수산화물의 첨가가 남은 음식물의 보관 중 성상변화에 미치는 영향
이현준,김현섭,정하연,강수원,기광석,조광근,조재순,홍중산,김명국,이홍구,최윤재,Lee, H.J.,Kim, H.S.,Jeong, H.Y.,Kang, S.W.,Ki, K.S.,Cho, K.K.,Cho, J.S.,Hong, Z.S.,Jin, M.G.,Lee, H.G.,Choi, Y.J. 유기성자원학회 2002 유기물자원화 Vol.10 No.3
이 시험은 현재 우리나라 환경문제 중 하나로서 큰 오염원으로 인식되고 있는 남은 음식물의 재활용과 사료로서의 이용성을 높이기 위한 보존재로서 MHA의 적정 사용량을 알아내기 위하여 실시하였다. 소규모 식당에서 발생하는 남은 음식물을 수집하여 methionine 수산화물(MHA)을 처리하여 $40^{\circ}C$ 인큐베이터안에서 40일동안 변화를 관찰하였다. MHA를 원물중량의 1%, 3%, 5% 첨가구를 두고 대조구와 비교하였다. 염도는 MHA 첨가량이 많아질수록 증가하는 경향이 있었으며(p<0.05) pH는 MHA의 첨가량이 많아질수록 낮아지는 경향을 보였다(p<0.05). 휘발성지방산함량에 있어서는 대조구는 30일 이후에 증가하는 경향을 보였고 MHA처리구는 1, 3, 5% 첨가구 모두 15일차까지 감소하고 이후 일정한 함량을 나타내었다. 암모니아태 질소함량은 MHA의 첨가수준이 높을수록 떨어지는 경향을 보였다(p<0.05). 시료의 외관상 변화는는 대조구에서 표면 곰팡이 발생과 연화정도가 심하게 나타내었으며, MHA 첨가구에서는 1%구에서는 표면의 얇은 곰팡이는 나타내었으나 내부는 변화를 보이지 않아 남은음식물의 고온상태에서의 변패 변질 방지를 위해 MHA를 1% 이상 첨가하면 효과가 있는 것으로 나타내었다. This study was carried out to improve the utilization of food wastes known as major source of environmental pollution and to determine proper additional levels of methionine hydroxy analogue(MHA) on food wastes. 1%, 3% and 5% of MHA were added to 3 groups of food wastes gathered from small cafeteria. Each group has 3 reiteration. NaCl contents were tended to get higher as addition levels increased(p<0.05). And pH values were tended to get lower as additional levels increased(p<0.05). $NH_3-N$ contents were significantly decreased as additional levels increased(p<0.05). Total VFA(Volatile Fatty Acid) content was increased after 30 days of storage on control. On MHA treatments(1, 3, 5%), total VFA contents were decreased until 15 days of storage, and kept low values until the end of experiment. In terms of superficial change, mildew covered all over control, and covered by thin mildew on the surface of 1% MHA treatment. No superficial change found on 3% and 5% MHA treatments. In conclusion, over 1% addition of MHA was effective to prevent the decay and degeneration of food wastes.
Porcine Ryanodine Receptor 유전자의 Mutation 을 확인하기 위한 Double Tube Allele Specific Pimer PCR 의 이용
김철욱,조광근,최윤재,박외선,권은정 ( C . W . Kim,K . K . Cho,Y . J . Choi,W . S . Park,E . J . Kwon ) 한국축산학회 1998 한국축산학회지 Vol.40 No.3
A double tube allele specific PCR assay was developed for a rapid and accurate detection of the porcine Ryanodine receptor genotypes using the allele specific pimer PCR. The assay, a modification of Zinovieva` single tube allele specific(STAS) PCR, reduced significantly primer dieter artificial band and determined allelic variations in simple, accurate and cost-effective ways. PCR primers were designed as followed ; two allele-specific `internal` primers[Int 1 for the normal allele N 5`-AGTAATGAGATCTTGGTTGGA(G→T)CG-3`, Int 2 for the mutant allele n : 5`-GTGCAATGGTGTGGCCG (T→C)GT-3`] were recognized with their 3`nucleotide the allelic variation. In order to increase the allele specific annealing an additional mismatch at position 3 from the 3` end of each primer was introduced. The common `external` primers(Ext 1, Ext 2) were located at different distances from the point mutation(nt 1843, C→T), so that different fragment lengths were generated for each allele. In the case of the gene, in normal(NN) and mutant (nn) homozygotes, only one fragment of 533 by or 823 bp, respectively, was amplified, whereas in heterozygotes (Nn) both fragments were detected. Independently from the genotype, an additional product(1312 bp) of the common `external` primers was amplified in. the single tube method. The use of the allele specific PCR for detecting allelic nucleotide exchanges offers some obvious advantages the presence of restriction endonuclease sites is not required and the allelic variations are visualized by simple agarose gel electrophoresis. As compared with the PCR-RFLP this modified method is simple, reliable and time and cost effective and thus can be applied in large scale to establish the program for testing and removal of the genes associated with the breeding of swine.
Pichia pastoris(Methylotrophic Yeast)를 이용한 항균성 양이온 펩타이드의 발현
조광근,최윤재,복진덕,이강우,곽성욱,우정희,성창근 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.4
Antimicrobial cationic peptides have been received increasing attention as natural antibiotics for their broad spectrum of antimicrobial activities and remarkably low cytotoxicity against normal mammalian cells. Pichia pastoris, a methylotrophic yeast, is an outstanding host for high degree of heterologous gene expression. Four candidates of antimicrobial cation peptides (CPs; Lactofemicin, Magainin, Protegrin-1 and Indolicidin) were expressed using pPIC9K-MPM vector containing AOXI promoter and MPM (modified Promagainin) as an acidic fusion partner. The MPM-CPs were expressed and induced by methanol induction method. The cell extracts, solubilized, were subjected to cyanogen bromide cleavage. SDS-PAGE (16.5% tricine) analysis showed the size of Protegrin-1 at 2.1kDa and of Indolicidin at 1.4kDa. The antimicrobial activity of Protegrin-1 or Indolicidin gene integrated transformant was observed by measuring clearing zones on 1% bacto-peptone agar plate against Gram-negative bacteria Escherichia coli XL-1 blue (10^5 CFU/㎖) or Gram-positive bacteria Staphylococcus aureus (10^5 CFU/㎖).