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유통 건고추 및 고춧가루의 아플라톡신 B1과 오크라톡신 A 오염도 조사
제갈승,김지형,주광식,정세진,나현주,조남규,이제만,김용희 한국식품위생안전성학회 2013 한국식품위생안전성학회지 Vol.28 No.3
2012년 2월부터 11월까지 인천 지역에서 유통된 건고추및 고춧가루 193건을 대상으로 아플라톡신 B1과 오크라톡신 A의 오염도를 조사하였다. Immunoaffinity column 및HPLC를 이용한 시험법은 모두 80% 이상의 회수율을 보였고, 아플라톡신 B1 및 오크라톡신 A의 검출한계는 각각0.13 μg/kg, 0.30 μg/kg였다. 오염도 조사를 한 결과 아플라톡신 B1은 17.1%의 검출율을 보였고 오크라톡신 A는 20.7%의 검출율을 보였으며, 아플라톡신 B1의 검출농도는 0.14~9.67 μg/kg였고, 오크라톡신 A의 검출 농도는 0.31~3.31 μg/kg였다. 이는 우리나라 식품공전 상의 기준인 10 μg/kg(아플라톡신 B1), 7 μg/kg(오크라톡신 A)보다는 낮은 수치로비교적 안전한 수준이었다. A survey of aflatoxin B1 and ochratoxin A was conducted on dried red pepper and red pepper powder. Total number of 193 samples were collected from local markets in Incheon. The presence of aflatoxin B1 and ochratoxin A was determined by high performance liquid chromatography (HPLC) with fluorescence detector using immunoaffinity column clean-up. The recovery rate of aflatoxin B1 and ochratoxin A were more than 80% and the limits of quantification were 0.13 μg/kg for aflatoxin B1 and 0.30 μg/kg for ochratoxin A. Aflatoxin B1 was detected in 33 samples (17.1%) with a range of 0.14~9.67 μg/kg and ochratoxin A was detected in 40 samples (20.7%) with a range of 0.31~3.31 μg/kg. These results show that the occurrence of aflatoxin B1 and ochratoxin A in dried red pepper and red pepper powder tested in this study is low compared with the standard in Korea Food Code (10 μg/kg as aflatoxin B1 and 7 μg/kg as ochratoxin A).
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유통 건고추 및 고춧가루의 아플라톡신 B<sub>1</sub>과 오크라톡신 A 오염도 조사
제갈승,김지형,주광식,정세진,나현주,조남규,이제만,김용희,Jegal, Seung,Kim, Ji-Hyeung,Joo, Gwang-Sig,Jung, Se-Jin,Na, Hyeon-Ju,Jo, Nam-Gyu,Lee, Jea-Man,Kim, Yong-Hee 한국식품위생안전성학회 2013 한국식품위생안전성학회지 Vol.28 No.3
2012년 2월부터 11월까지 인천 지역에서 유통된 건고추 및 고춧가루 193건을 대상으로 아플라톡신 $B_1$과 오크라톡신 A의 오염도를 조사하였다. Immunoaffinity column 및 HPLC를 이용한 시험법은 모두 80% 이상의 회수율을 보였고, 아플라톡신 $B_1$ 및 오크라톡신 A의 검출한계는 각각 0.13 ${\mu}g/kg$, $0.30{\mu}g/kg$였다. 오염도 조사를 한 결과 아플라톡신 $B_1$은 17.1%의 검출율을 보였고 오크라톡신 A는 20.7%의 검출율을 보였으며, 아플라톡신 $B_1$의 검출농도는 0.14~9.67 ${\mu}g/kg$였고, 오크라톡신 A의 검출 농도는 0.31~3.31 ${\mu}g/kg$였다. 이는 우리나라 식품공전 상의 기준인 10 ${\mu}g/kg$(아플라톡신 $B_1$), 7 ${\mu}g/kg$(오크라톡신 A)보다는 낮은 수치로 비교적 안전한 수준이었다. A survey of aflatoxin $B_1$ and ochratoxin A was conducted on dried red pepper and red pepper powder. Total number of 193 samples were collected from local markets in Incheon. The presence of aflatoxin $B_1$ and ochratoxin A was determined by high performance liquid chromatography (HPLC) with fluorescence detector using immunoaffinity column clean-up. The recovery rate of aflatoxin $B_1$ and ochratoxin A were more than 80% and the limits of quantification were 0.13 ${\mu}g/kg$ for aflatoxin $B_1$ and 0.30 ${\mu}g/kg$ for ochratoxin A. Aflatoxin $B_1$ was detected in 33 samples (17.1%) with a range of 0.14~9.67 ${\mu}g/kg$ and ochratoxin A was detected in 40 samples (20.7%) with a range of 0.31~3.31 ${\mu}g/kg$. These results show that the occurrence of aflatoxin $B_1$ and ochratoxin A in dried red pepper and red pepper powder tested in this study is low compared with the standard in Korea Food Code (10 ${\mu}g/kg$ as aflatoxin $B_1$ and 7 ${\mu}g/kg$ as ochratoxin A).
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도은염색법중(鍍銀染色法中) Slide 부착법(附着法)과 절편부유법(切片浮遊法)의 비교고찰(比較考察)
제갈승주 ( Jekal Seung Joo ),오근영 ( Keun Young Oh ) 대한임상검사과학회 1979 대한임상검사과학회지(KJCLS) Vol.11 No.1
Two kinds of silver impregnation methods are most widely used in routine histopathological laboratory. that is frozen section which requires careful handling, and processing and produces inconstant result and uncomfortable procedure, in addition the section is easily or frequently detached from the slide due to high alkalinity of silver particles. This may be overcome by carrying out steps of the procedure . by floating the paraffin section on the solutions. The procedure shows simple and no detachment from the slide, also it can get a clear background by deparaffinization.
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위점막에서 헤마톡살련 염색시간을 연장한 메이어헤마톡살린-에오신염색에 의한 Helicobacter pylori-likeorganism 검출률과 특수 염색법들과의 비교
제갈승주 ( Seung Joo Jekal ),차현희 ( Hyun Hee Cha ),김신무 ( Shin Moo Kim ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
Helicobacter pylori-like organism(HLO) is more easily visua1ized with specia1 stainings than with hematoxylin-eosin, but this is time-consumed, expensive to use the routine diagnostic workup. We performed to verify whether Mayer``s hematoxylin-eosin staining method with prolonged hematoxylin time compares favorably with other well established specia1 stainings in the detection of HLO in gastic biopsy. Fifty gastric biopsy specimens(48 from antrum, two from body) , routinely forma1in f1xed and paraff1n wax embedded, from gastritis patients were stained with modified Harris`` hematoxylin-eosin(m-Harris HE) and modified Mayer``s hematoxylin-eosin m-Mayer HE) with prolonged hematoxyrlin time(10min), Warthin-Starry, Genta, AgNOR and EI-Zimaity staining. The detection rate for HLO was examined by divide into three groups (mild, moderate, severe) according to the degree of inflammation in the biopsy specimens. Density of HLO was scored from 0 to 5 by Genta``s c1assification. Using Warthin-Starry as a standard, positivity for HLO was 52% (26/50). Relative sensitivity for Warthin-Starry staining was 100% with m-Mayer HE, 96% with Genta, 96% with EI-Zimaity, 92% with AgNOR, and 80% with m-Harris HE staining. Relative sensitivity of HLO density for Warthin-stany stainings was lower at low (grade 1) density(Genta, 96%; EI-Zimaity. 96%; AgNOR, 33%, routine Harris HE, 0%) than at high(grade 2 to 5) density except case with m-Mayer HE(100%). Detection rate and density of HLO also appeared a significantly high according to increase the degree of inflammation. m-Mayer HE staining has a sensitiviη comparable to thoes of Warthin-Starry and Genta staining and is simple, inexpensive compared with the other staining methods in detecting HLO. Therefore, it is vety useful and practica1 for identifying HW in routine gastric biopsy specimens.
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스트렙토조토신 유도 당뇨 흰쥐에서 전기자극이 상처치유와 피부 비만세포에 미치는 영향
제갈승주 ( Seung Joo Jekal ),이경선 ( Kyung Sun Lee ),정옥봉 ( Ok Bong Chung ),이재형 ( Jae Hyoung Lee ) 대한임상검사과학회 2008 대한임상검사과학회지(KJCLS) Vol.40 No.2
The aim of this study was to investigate the effect of electrical stimulation on healing of impaired wound and alteration of mast cells in experimental diabetic rats. Thirty male Sprague-Dawley rats were divided into three groups : incision (control), diabetes+incision (diabetes) and diabetes + incision + electrical stimulation (D/ES). Diabetes was induced in rats by streptozotocin (STZ) injection (60 mg/kg, one time) and 20 mm length incision wounds were created on the back after shaving hair. The electrical stimulation rats were treated with a current intensity of 30~50 V at 120 pps and 140 μs for 10 days from 3 days after STZ injection. The lesion and adjacent skin tissues were fixed with 10% buffered formalin, embedded with paraffin. For wound healing analysis, hematoxylin-eosin (HE) and picrosirius red staining were performed. Mast cells (MC) were stained with toluidine blue (pH 0.5) and quantified at ×200 using a light microscope. The density of keratinocyte proliferation and microvessels in skin tissues were analyzed using a computerized image analysis system on sections immunostained with proliferative cell nuclear antigen (PCNA) and α-smooth muscle actin (α-SMA), respectively. The results showed that the wound healing rate, collagen density and neoepidermis thickness, density of PCNA-positive cells and density of α-SMA-positive vessels were significantly higher in D/ES rats than in diabetic rats. The density of MCs and degranulated MCs in D/ES rats were also significantly higher than those in diabetic rats. These findings suggest that the electrical stimulation may promote the tissue repair process by accelerating collagen production, keratinocyte proliferation and angiogenesis in the diabetic rats, and MCs are required for wound healing of skin in rats.
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사염화탄소 유도 간섬유증 발생에 었어서 비만세포와 근섬유모세포의 상호 관련성
제갈승주 ( Seung Joo Jakal ) 대한임상검사과학회 2001 대한임상검사과학회지(KJCLS) Vol.33 No.2
Hepatic fibrosis is known to be developed by producing the extracellular matrices from myofibroblasts(a-SMA postive cells) that are transformed from hepatic satellite cells. The development of the fibrotic process is thought to be mediate by various fibrogenic mediators. Recent1y, several studies have suggested that mast cells participate in the development of the hepatic fibrosis in human liver diseases and in rodent models. But The close relation between mast cells and myofibroblasts in the development of hepatic fibrosis remains still llllclear. In this study We have investigated the change of collagen content, the disπibution and density of mast cells and myofibrobalsts during 2, 4, 6-week after carbon tetrachloride treatment in rat. Twenty male Wistar rats were divided into four groupsincluding control. Hepatic fibrosis was induced by intraperitoneal injection of carbon tetrachloride(CC14) in rats. πle degree of fibrosis was evaluated by measuring the hydroxyproline content(ugjg tissue) of the liver as an index of the collagen content and by using a numerical scoring system for grading hepatic fibrosis in the liver sections stained with Masson trichrome and Gomori reticulum, respectively. The density of mast cells (number/mmj was determined by cOllllting mast cells using a computerized image analyser system in liver sections stained with toluidine blue and alcian blue-safranin O, respectively. Also, πle morphological relation between mast cells and myofibloblasts was examined by elecπon microscopy. πle c이lagen content was significant1y increased at 4 to 6week after CC4 treatment compared with control rats. These results showed significant correlations (Spe따man’ s,r=O.76, p<0.001) with those of a numerical scoring system. In control rats, mast cells were found principally in portal areas, and their average density was 4.76 i= 2.23/ MM2 in liver sections stained with alcian blue-safranin O. Whereas in CC4-treated rats mast cells were diffusely distributed in fibrous septa and fibrous capsule on liver surface and were significantly increased at 2(8.86 i= 1.94/mnf), 4(12.26 i= 3.59/mm2 to 6week(28.09 i= 12.74/ mm2)after CC4 treatment compared with control rats. In cαltrol rats, myofibroblasts were not found in portal areas and observed only Q-SMA positive staining on vessel walls in portal areas. In CC4-treated rats, myofibroblasts were occasionally found at 2week after CC4 treatment in portal areas, fibrous septa and liver parencyma, but were statistically not significant compared with control rats. Myofibrobasts were significantly increased at 4 to 6week after CC4 treatment. U1trastructurally, mast cel1s were observed in close contact with myofibroblasts in fibrous septa and showed the various stages of degranulation. πlese results suggested that mast cell may play role in deve10pment of hepatic fibrosis, probably because of the production of extracellular maπix components by myofibroblasts which activate by some mediators released from mast cells.
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