안중근의사 유적지 및 항일독립운동 역사현장 탐방기

정화현 한국외교협회 2015 외교 Vol.112 No.0

안중근의사 유적지 및 항일독립운동 역사현장 탐방기

정화현 한국외교협회 2015 계간 외교 Vol.- No.112

RBL-2H3 세포에서 Hesperidin의 Th2 사이토카인 발현 억제 효과

정화현,표명윤,김순례 한국생약학회 2013 생약학회지 Vol.44 No.2

Hesperidin (HES), a flavonone glycoside isolated from the citrus fruits such as lemons and oranges, has been reported to have many biological properties including antiinflammatory, antioxidant, and antiallergy activities. In this study, we focused on the action of HES modulating Th2-associated cytokines such as IL-4 and IL-13 expression in PMA/ionomycin (PI)-stimulated rat basophilic leukemia (RBL-2H3) cells. The production of IL-4 and IL-13 was quantified by ELISA and the mRNA expression was detected by using RT-PCR assay. In addition, western blot analysis was performed to determine the transcription factors involved in the cytokine expression. We found that HES significantly decreased PI-induced IL-4 and IL-13productions and also decreased the level of mRNA in a dose-dependent manner. Furthermore, western blot analysis of the transcription factors implied that HES down-regulated the protein level of c-Jun and c-Fos, which are the activating protein 1 (AP-1) family and nuclear factor-kappaB (NF-κB) characterized as a transcription factors related to the Th2-associated cytokine expression. Taken together, our data showed that the action of HES responsible for antiallergy activities is based on suppression of Th2-associated cytokines through inhibition of AP-1 and NF-κB transcription factors.

Schizandrin에 의한 호염구와 비만세포의 Th2 관련 사이토카인 발현 억제

정화현,윤수정,표명윤 한국생약학회 2012 생약학회지 Vol.43 No.3

Schizandrin (SZ), was isolated from the fruit of Schizandra chinensis, has been reported to have many biological properties, including anti-inflammatory and antitumor activities. However, its anti-allergic effects are not completely elucidated. We focused on the anti-allergic effects of SZ in PMA/ionomycin (PI)-induced rat basophilic leukemia (RBL-2H3) cells and P815 mast cells. Cytokines (IL-4, IL-13), synthesized by basophils and mast cells, are implicated in pathological conditions such as asthma and allergy. The production of IL-4 and IL-13 was quantified by ELISA and the mRNA expression was detected by using RT-PCR assay. In this study, we found that SZ did not show cytotoxic effect at up to 100 µM on RBL-2H3 cells and mast cells. In addition, SZ inhibited the production of IL-4 and IL-13 and also decreased the level of mRNA in PI-induced RBL-2H3 cells and mast cells in a dose-dependent manner. Thus, we suggest that SZ may have the effect on preventing allergic disorders by inhibiting IL-4 and IL-13 cytokines

RBL-2H3 세포에서 Cyanidin-3-glucoside의 Th2 사이토카인 발현 억제 효과

정화현,윤수정,표명윤 대한약학회 2013 약학회지 Vol.57 No.5

Cyanidin-3-glucoside (C3G), an anthocyanin, is one of the most widespread dietary flavonoids. We investigated the effects of C3G in PMA/ionomycin (PI)-induced RBL-2H3 cells. C3G inhibited the production of IL-4 and IL-13 and also decreased the level of mRNA in a dose-dependent manner. Furthermore, western blot analysis implied that C3G down-reg- ulated the protein level of c-Jun, NF-ATc1 and NF-κB but not c-Fos. Taken together, we suggest that C3G may have sup- pressive effects on Th2 cytokines and will be studied further to develop as functional foods that help alleviate allergy symptoms.

Identification and functional characterization of TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factor genes in Pak-choi (Brassica rapa ssp. chinensis)

정화현,김영천,이정환 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.3

TEOSINTE BRANCHED1/ CYCLOIDEA/PROLIFERATING CELL FACTORS (TCP) are a group of plant-specific genes that encode transcription factors and play essential roles in plant growth and development. Of these genes, Arabidopsis TCP5 (AtTCP5), TCP13 (AtTCP13), and TCP17 (AtTCP17) regulate flowering time, one of the important factors of plant development. Controlling flowering time of Pak-choi (Brassica rapa ssp. chinensis) is a method of improving plant growth conditions, as vegetative tissues such as leaves are not produced after floral transition. To investigate functional conserva- tion in three Pak-choi TCP genes homologous to AtTCP5, AtTCP13, and AtTCP17 in the regulation of flowering time, we isolated BcTCP5, BcTCP13, and BcTCP17 from a Pak-choi cultivar. The deduced amino acid sequences of the selected TCP genes were 65–82% identical to the three aforementioned AtTCP proteins. The expression levels of BcTCP5, BcTCP13, and BcTCP17 were spatially different and were unaffected by vernalization treatment. The three BcTCP proteins also localized in the nucleus, and interacted with Arabidopsis FLOWERING LOCUS D (AtFD) and PHTOCHROME INTERACTING FACTOR4 (AtPIF4). Overexpression of BcTCP17 in Arabidopsis plants led to an early flowering phenotype by up-regulating endogenous Arabidopsis FOWERING LOCUS T (AtFT) and APETALA1 (AtAP1) expression, suggesting that BcTCP17 regulates flowering time in Arabidopsis. Our results indicated that the three BcTCPs isolated from Pak-choi plants are the equivalents of corresponding AtTCPs, and that BcTCP17 may be a candidate to be used in the regulation of flowering time in Pak-choi plants utilizing the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system.

첨가물에 의한 봉독의 안정화 및 안전성

배영현,이종환,김해솔,김호선,서창용,김노현,이진호,하인혁,김미령,정화진,이인희,김민정,김은지,이재웅 대한침구의학회 2015 대한침구의학회지 Vol.32 No.3

Objectives : Previous studies have shown that the amount of melittin, the main active ingredient in bee venom pharmacopuncture, tends to decrease substantially with time during pharmacopuncture manufacture. This study aimed to assess whether the stability of bee venom pharmacopuncture improved with pharmacopuncture additives. Methods : Components were measured using high performance liquid chromatography. Acute toxicity and antigenicity tests by subcutaneous and venous routes were conducted at Korea Pharmaceutical Test & Research Institute and mortality, adverse reactions, and body weight changes were assessed. Results : Stability tests using additives revealed that bee venom without additives was most stable. Bee venom pharmacopuncture without additives was further tested for toxicity in subcutaneous and venous administration in mice and no changes pertaining to toxicity were found over the testing period. Conclusions : Bee venom pharmacopuncture without additives was found to be most stable, and further, it did not show toxicity or antigenicity in subcutaneous and venous administration in mice.

고성능 탄소섬유 제조를 위한 폴리아크릴로니트릴 기반 공중합 고분자 합성 및 전구체 섬유 방사

주혜진,한민정,송경현,전창범,정화경,김민정,채한기 한국복합재료학회 2022 Composites research Vol.35 No.2

The performance of carbon fiber is important for the production of these high-quality polymer composite materials such as CFRP (Carbon Fiber Reinforced Plastic). For this purpose, it is essential to use an optimized spinning process for improving the mechanical, physical, and structural properties of the precursor fiber, which greatly affects the properties of the carbon fiber, and the use of a suitable precursor polymer. In this study, the content of MAA (Methacrylic Acid), MAA injection time, and concentration of AIBN (2,2′-Azobis(2-methylpropionitrile)) were set as parameters for the polymer synthesis process, and Poly(AN-co-MAA) (poly(acrylonitrile-co-methacrylic acid)) was polymerized by solution polymerization. Poly(AN-co-MAA) with a molecular weight of 305,138 g/mol and an MAA ratio of 4.2% was dissolved in DMF (N,N- dimethylformamide) at a concentration of 16.0 wt%, and then a precursor fiber was prepared through dry-jet-wet spinning. The precursor fiber had a tensile strength of ~1.06 GPa and a tensile modulus of ~22.01 GPa, and no voids and structural defects were observed on the fiber. 탄소섬유의 성능은 탄소 섬유 강화 플라스틱 (CFRP)과 같은 고품질 고분자 복합재료에 매우 중요하다. 이를 위해 탄소섬유 물성에 큰 영향을 주는 전구체 섬유의 기계적, 물리적, 구조적 특성을 개선할 수 있는 최적화된 방사공정과 이를 위한 적합한 전구체 공중합 고분자를 사용하는 것은 필수적이다. 본 연구에서는 메타크릴산 (MAA)의 함량과 주입시간, 2,2'-아조비스(2-메틸프로피오니트릴) (AIBN)의 농도를 합성공정 변수로 설정하였으며, 용액 중합법 (solution polymerization)에 의해 Poly(AN- co-MAA)가 합성되었다. 305,138g/mol의 분자량과 4.2%의 MAA 비율을 가지는 Poly(AN-co-MAA)를 N,N-디메틸포름아미드 (DMF)에 16.0wt% 농도로 용해시킨 후 기격습식방사법 (dry-jet-wet spinning)으로 전구체 섬유를 제조하였다. 섬유의 인장강도는 ~1.06 GPa, 인장탄성률은 ~22.01 GPa였으며, 섬유에서의 공극 및 구조적 결함은 관찰되지 않았다.

도로의 경사도에 따른 자율주행 가속도 추정 모델

박경욱,허명선,오영철,한지형,정화현,유병용 대한임베디드공학회 2021 대한임베디드공학회논문지 Vol.16 No.6

Autonomous vehicles are divided into an upper controller that calculates control value through cognitive judgment and a lower controller that appropriately transmits its control value to an actuator. Here, the longitudinal control in a lower controller has a problem as the road slopes due to the property of the Acceleration sensor to output the acceleration as the slope of the device. Therefore, in this paper, a sigmoid function is proposed to determine the slope to compensate for this problem. Through the experiment, Checked performance by comparing the existing table model with the proposed model.

다분광 영상데이터를 이용한 배추 내건성 평가

이준호,이상덕,김진희,정화현,장윤아 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-