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정상호(Sang Ho Chung),고경원(Kyung Won Ko),홍주봉(Choo Bong Hong),최인성(In Sung Choi),정태화(Tae Wah Chung) 한국식물학회 1989 Journal of Plant Biology Vol.32 No.1
We have developed a plasmid vector, pKCHl, for the purpose of higher plant transformation. It contains the promoter region of cauliflower mosaic virus 35S transcript (P_35s) and the terminator region of nopaline synthase gene (Tnos) with unique cloning site, Bam HI and Xba Ⅰ, between them. After inserting a foreign gene at the cloning site, P_35s-foreign gene-Tnos cassette can be recovered by using a restriction enzyme Hind Ⅲ.