< 전시-P-77 > P inus densiflora 목부로부터 항산화 물질 분리를 위한 열처리 적용 및 항산화 특성 비교

정지영 ( Ji Young Jung ),하시영 ( Si Young Ha ),박재현 ( Jai Hyun Park ),이동환 ( Dong Hwan Lee ),최지원 ( Ji Won Choi ),양재경 ( Jae - Kyung Yang ) 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.1

Lignocellulosic materials, which consist mainly of cellulose, hemicellulose and lignin, are among the promising renewable feedstock for the production of antioxidant compounds. The thermal treatment of lignocellulosic materials intends primarily the selective breakdown of hemicelluloses to obtain sugar or sugar oligomer. Cell wall-linked phenolic compounds are also solubilized during this type of treatments. The aim of the present work was to investigate and compare phenolic compounds and antioxidant activity of ethanol extracts (90% ethanol, 30 ℃, 100 rpm, 24 hours) of P. densiflora stems obtained after thermal treatment. Thermal treatment of P. densiflora stems was either treated by steam treatment (180 ℃, 10 min), steam explosion treatment (225 ℃, 10 min) or roasting treatment (225 ℃, 10 min). Total phenolic compound was determined spectrophotometrically and antioxidant activity of the samples was assayed through FRAP (ferric reducing antioxidant power) and DPPH radical scavenging assay. The ethanol extracts of steam exploded P. densiflora stem had rapidly higher total phenolics content compared to the extracts of steam treated and roasting treated P. densiflora stem. Also, the ethanol extracts of steam exploded P. densiflora stem, exhibited higher antioxidant activity than ethanol extracts of steam treated and roasting treated P. densiflora stem. Based on our results, the steam explosion treatment seems to be the best treatment for separation of phenolic compound, followed by the steam treatment and roasting treatment for the production of antioxidant compounds.

열처리된 목질계바이오매스로부터 페놀성 화합물 분리를 위한 공융 용매 기반 추출 최적화에 있어서 Box-behnken과 Central composite design 설계의 비교

정지영 ( Ji Young Jung ),하시영 ( Si Young Ha ),박재현 ( Jai Hyun Park ),이동환 ( Dong Hwan Lee ),최지원 ( Ji Won Choi ),양재경 ( Jae-Kyung Yang ) 한국목재공학회 2020 한국목재공학회 학술발표논문집 Vol.2020 No.1

Deep eutectic solvents, environmentally friendly “green”solvents, have recently been applied extensively in several areas of chemistry, including the preparation of inorganic materials, organic synthesis, biochemistry, and analytical chemistry. There are an increasing number of studies in extraction and separation media for bioactive plant compound, including flavonoids, catechin, phenolic acid, terpenoids, and saponin. For the optimization of liquid-solid extraction processes, the response surface methodology is usually applied in combination with the full factorial design (FFD), central composite design (CCD) or Box-Behnken design (BBD) serving for the data collection. The present study deals with comparing the performances of CCD and BBD, which are used in combination with the response surface methodology (RSM) for the optimization of the phenolic compound extraction by deep eutectic solvent with respect to deep eutectic solvent concentration (1.6-3.2 g), time (120-240 min), solvent-to-sample ratio (20:1-60:1) and temperature (40-80℃). The optimum range condition was deep eutectic solvent concentration (2.1-2.2 g), time (182-200 min), solvent-to-sample ratio (36-47) and temperature (60-70℃). All two methods were efficient in the statistical modeling and optimization of the influential process variables and led to almost the same optimal process conditions and predicted phenolic compound content. Having better statistical performances and being economically advantageous over the CCD with repetition, the BBD combined with the RSM is recommended for the optimization of liquid-solid extraction processes.

ICT 기술을 이용한 토종다래 재배지의 토양환경인자 분석 및 결정트리 적용

하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),박재현 ( Jai Hyun Park ),이동환 ( Dong Hwan Lee ),최지원 ( Ji Won Choi ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.2

The other edible and very promising Actinidia species is Actinidia arguta (Siebold & Zucc.) Planch. ex Miq., also known as kiwiberry, hardy kiwi, baby kiwi, or mini kiwi. This exotic species is very interesting and promising given the horticultural advantages it has over kiwifruit, especially its high frost hardiness (down to -30 ℃ in midwinter) and relatively short vegetation period. To the best of our knowledge, there have been few experiments studying the effect of soil properties on mini kiwifruit productivity as well as the sugar content of A. arguta fruit. The variety of cultivar and expansion in arable farming has emphasized a need for more specific information related to factors that affect A. arguta productivity and sugar content of the fruit in the region. We analyzed various soil properties at experimental sites in South Korea. A Pearson’s correlation analysis was performed between the soil properties and sugar content or productivity of A. arguta. Further, a decision tree was used to determine the optimal soil conditions. The difference in the fruit size, sugar content, and productivity of A. arguta across sites was significant, confirming the effects of soil properties. The decision tree analysis showed that a soil C/N ratio of over 11.49 predicted a sugar content of more than 7 °Bx at harvest time, and soil electrical capacity below 131.83 μS/cm predicted productivity more than 50 kg/vine at harvest time. Our results present the soil conditions required to increase the sugar content or productivity of A. arguta, a new A. arguta cultivar in South Korea.

액체배양된 P aecilomyces japonica로부터 cordycepin 분리를 위한 분획용매의 영향

하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),박재현 ( Jai Hyun Park ),이동환 ( Dong Hwan Lee ),최지원 ( Ji Won Choi ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2020 한국목재공학회 학술발표논문집 Vol.2020 No.1

Cordycepin is a nucleoside analog (3′-deoxyadenosine), which is the major active constituent of Cordyceps militaris and was first reported as a metabolite isolated from a culture broth of P. japonica. To date, cordycepin has been demonstrated to have many pharmacological activities, such as anti-tumor, anti-metastatic immunomodulatory and anti-inflammatory activities, and supply of a large amount of purified cordycepin is essential to the developments of its novel pharmacological use as well as its clinical trials. However, there are very few reports on commercially feasible procedures for isolation and purification of cordycepin. From the discovery of corycepin to the present, the purification methods have been based on column chromatography. The present study was carried out to develop an extraction strategy for cordycepin and to isolation of cordycepin from Paecilomyces japonica in submerged culture. Solvent-solvent extraction method was used to extract cordycepin from submerged culture of P. japonica. Crude concentrated extract of fermented broth was sequentially partitioned with hexane, chloroform and n-butanol. Further we tried to isolation of cordycepin from P. japonica using Sephadex-LH20. As a result, it was confirmed that cordycepin was detected when the liquid culture medium was fractionated using n-butanol. It was also confirmed that a single cordycepin was detected when separated by sephadex column chromatography using 80% methanol as a solvent. At this time, the detection of cordycepin was analyzed by HPLC. It is hoped that these results will be used as basic data for cordycepin separation from Cordyceps mycelia.

< 구두-D-03 > LED (Light Emitting Diode) light가 Cordyceps militaris 균사체의 생장 및 cordycepin 함량 증대에 미치는 영향

하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),박재현 ( Jai Hyun Park ),이동환 ( Dong Hwan Lee ),최지원 ( Ji Won Choi ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.1

Mushroom is an abundant source of a wide range of useful natural products with biological activities. Because field cultivation of ushrooms takes a long time to grow and low productivity of bioactive compounds, submerged cultivation of mushrooms is viewed as a promising alterative for producing valuable substances. Cordyceps militaris (C. militaris), a caterpillar-shaped Chinese traditional medicinal mushroom, is usage as a crude drug, it has been extensively used as folk tonic food or invigorant since ancient times. This mushroom produces an important bioactive compound, cordycepin (3′-deoxyadenosine), which is a nucleoside analogue. Cordycepin is reported to possess many interesting biological and pharmacological activities, including immunological stimulating, anti-cancer, anti-virus and anti-infection activities. Previous work reported the isolation of cordycepin from liquid culture medium of C. militaris and its pharmacological functions. But, as far as we know, there is limited knowledge about the LED (Light Emitting Diode) condition for cordycepin production by C. militaris. In this paper, the effects of LED were focused in order to improve the cordycepin production by submerged cultivation of C. militaris. For this experiment, mycelial cultivation was performed in a shaking incubator at 24 ℃, 100 rpm for 5 days, and the medium was sabouraud dextrose broth (pH 5.6). The LED light mixed red and green, LED light mixed red and blue and LED light mixed green and blue were used for the LED, and dark culture and fluorescent lamp were used as the control. This results, C. militaris showed the highest mycelial weight when green light was irradiated, on the other hand, when irradiated with blue light, the content of cordycepin is about 7 times higher than that of fluorescent lamp in the cultures of C. militaris. In addition, the highest content of cordycepin was observed when irradiated for 6 h/day for 3 days. Interestingly, mycelial weight and cordycepin content were inversely related. The information obtained is considered fundamental and useful to the development of C. militaris cultivation process for efficient production of cordycepin on a large scale.

액체배양된 Paecilomyces japonica의 코디세핀 함량에 대한 LED와 우드밀이 포함된 배지의 영향

하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),박재현 ( Jai Hyun Park ),이동환 ( Dong Hwan Lee ),최지원 ( Ji Won Choi ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2020 한국목재공학회 학술발표논문집 Vol.2020 No.1

Paecilomyces japonica has been widely used based on folklore, not scientific studies. Cordycepin, a metabolite of P. japonica, has been showed to inhibit the growth of various tumor cells. Previous work reported the isolation of cordycepin from a liquid culture medium of P. japonica and its pharmacological functions. However, to the best of our knowledge, information on the light-emitting diode (LED) condition and wood meal-based media for cordycepin production by P. japonica is lacking. This study focused on the effects of a LED and wood meal based-media to improve cordycepin production by the submerged cultivation of P. japonica. For this experiment, mycelial cultivation was performed in a shaking incubator at 24 ℃ and 100 rpm for 5 days, with a medium of sabouraud dextrose broth (pH 5.6) with wood meal (P. densiflora). Red-green, red-blue, and green-blue LEDs were used for the experiments; a dark culture and fluorescent lamp were used as the control. P. japonica showed the highest cordycepin content when red-blue light was irradiated, the content of cordycepin was approximately 6 times higher than that with the fluorescent lamp in the cultures of P. japonica. In addition, the highest content of cordycepin was observed when cultivated in media with 20 g/L of 1% NaOH pretreated wood meal (100 mesh) under red-blue light.The combined application of LED and wood meal based-media has been shown to show synergistic effect for increasing cordycepin content from P. japonica. The information obtained is fundamental and useful to the development of P. japonica cultivation processes for the efficient production of cordycepin on a large scale.

< 전시-P-103 > 목질 바이오매스를 이용한 옥상 녹화용 식생기반재의 물리·화학적 특성 및 식물 독성 평가

박재현 ( Jai Hyun Park ),하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),이동환 ( Dong Hwan Lee Jae-kyung Yang ),양재경 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.1

1990년대 후반부터 옥상녹화에 대한 연구가 시작되었으며, 현재에 이르러서는 경량의 식생기반재를 사용하여 건물 옥상에 실제 숲과 같은 ‘Eco-region’을 만드는 것에 대한 관심이 모아지고 있다. 이와 함께, 하수 슬러지, 농업 폐기물 등을 이용한 옥상 녹화용 식생기반재 개발에 대한 연구들이 많이 이루어지고 있다. 본 연구에서는 목질 자원을 이용하여 식생기반재를 제조하였고, 제조된 식생기반재의 옥상 녹화 이용가능성을 알아보기 위해 물리·화학적 특성 및 식물 독성을 평가하였다. 식생기반재는 목질원료, 피트모스, 펄라이트, 녹화용 비료, 녹화용 접착제, 액상 첨가제를 혼합하여 제조하였다. 토양을 대조구로 하여 시판 제품과 제조된 식생기반재의 물리·화학적 특성을 비교하고, 2종의 식물을 (벌노랑이, 월동춘채) 사용하여 제조된 식생기반재의 식물 독성 평가 (Germination index) 를 확인하였다. 실험 결과, 제조된 식생기반재의 물리적 특성 중 수분함량, 가비중, 공극률에서 (각각 26.5%, 0.1 g/cm3, 78.5%) 토양과 시판제품보다 식물 생장에 적합하였고, 화학적 특성에서는 유기물 함량, CEC, K 의 함량이 (각각 82.5%, 16.9 cmolc/kg, 88.9 cmolc/kg) 토양과 시판제품보다 식물 생장에 적합한 것을 확인하였다. 그리고 식물 독성 평가 결과, 벌노랑이, 월동춘채의 germination index는 각각 84.0%, 76.7%, 65.7% 인 것을 확인하여 식물에 독성을 미치지 않는 것으로 확인되었다. 결론적으로, 목질 자원을 이용하여 제조된 식생기반재는 식물 생장에 적합한 물리·화학적 특성을 가지고 있으며, 식물에 독성을 미치지 않으므로 옥상 녹화를 위해 사용할 수 있을 것으로 확인되었다.

취수원 수질예측을 위한 통계적 분석 기법 활용

이호현(Lee Ho Hyun),김대욱(Kim Dae Wook),정지영(Park Si Hyun),신강욱(Shin Gang Wook),홍성택(Hong Sung Taek) 한국통신학회 2020 한국통신학회 학술대회논문집 Vol.2020 No.8

< 전시-P-105 > 습지 식생복원을 위한 Bacillus subtilis를 포함하는 목질계 인공토양 제조 연구

김지수 ( Ji-su Kim ),정지영 ( Ji Young Jung ),하시영 ( Si Young Ha ),박재현 ( Jai Hyun Park ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.1

본 연구는 습지 식생복원을 위한 B. subtilis를 포함하는 목질계 인공토양을 제조하기 위해 수행되었다. 피트모스, 퍼라이트, 고온고압처리된 신갈나무 및 B. subtilits를 포함하는 미생물원료를 3: 1: 3: 3 (w/w/w/w)로 혼합하여 인공토양을 제조하였고 야외포트 조성 및 성능평가를 위하여 평지의 지면에 자라나 있는 식물을 제거하고, 평탄화 작업을 한 후 7.5 cm × 7.5 cm × 7.5 cm 정사각형 모양의 육면체 포트에 습지토양과 제조된 인공토양을 각각 0%, 25%, 50% 75% 및 100% 비율로 혼합하여 현장적용 하였으며, 3개월간 물리·화학적특성 및 생육특성을 관찰하였다. 그 결과 일반적으로 식물생육에 적합한 용적밀도가 0.4 g/㎤ 이하임을 고려할 때, 제조된 인공토양은 현장적용 후 용적밀도가 0.08 ~ 0.39 g/㎤로 나타나 식물생육에 적합한 것으로 판단되었고 식물생장에 적합한 공극률이 85%이상인 것을 고려할 때 제조된 인공토양에 함유된 퍼라이트로 인하여 85.3 ~ 92.6%의 일정 공극률을 나타내어 식물생육에 있어서 장기간 보비력 및 보수력에 도움을 줄 수 있을 것으로 판단되었다. 또한 제조된 인공토양 100%와 습지토양에 제조된 인공토양을 50%, 75% 혼합하였을때 식물생육에 적합한 수분보유력(60%이상)을 나타내었고 제조된 인공토양의 혼합비율이 증가할수록 전기전도도 수치가 높아지는 경향을 나타냈으나 식물생육에 적합한 토양의 적정 수치인 0.5 ds/m 이하를 나타내어 식물생육에 큰 영향을 미치지 않을 것 으로 판단되었다. 제조된 인공토양에 대한 식물생육특성을 분석한 결과 습지토양에 제조된 인공토양이 25% 혼합되었을 때 대조구의 싸리발아율(13.8%)보다 높은 15.8%의 발아율을 나타냈으며, 잔디 발아율에 있어서는 제조된 인공토양이 25 ~ 100% 혼합 되었을 때 54.7 ~ 59.2%의 발아율을 나타내 51.8%의 대조구보다 높은 발아율을 나타냈고 낭아초에 대해서도 제조된 인공토양이 25%, 50%, 100% 혼합되었을 때 6.7 ~ 7.8%로 나타나 5.7%인 대조구보다 높은 발아율을 나타냈다. 따라서 본 연구를 통해 제조된 B. subtilis를 포함하는 목질계 인공토양은 습지 식생복원을 위한 인공토양으로서 활용가능 할 것으로 판단된다.

열처리 우드 칩을 이용한 인공상토의 제조 및 상추의 생장 평가

이동환 ( Dong Hwan Lee ),정지영 ( Ji Young Jung ),하시영 ( Si Young Ha ),박재현 ( Jai Hyun Park ),최지원 ( Ji Won Choi ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.2

상토는 식물체를 지지하고 작물의 생육에 필요한 각종 양분과 수분을 공급해주는 물질로서 육묘에 있어 가장 기본이 되는 물질이다. 일반적인 상토로 가장 많이 이용되는 피트모스는 주로 캐나다와 북유럽 지역에서 채취되기 때문에 전량 수입에 의존하는 실정이다. 이에 따라 가격이 높아질 뿐만 아니라 피트모스 제조에 장기간이 소요된다는 단점이 있다. 따라서 최근 피트모스를 대체할 수 있는 원료를 개발하기 위한 연구가 주목받고 있다. 우드 칩은 사업장 또는 간벌 후 다량 발생되고 있으며 처리를 위해 대부분이 소각되는데, 이는 공기중에 유해가스를 유발시킨다. 따라서 본 연구에서는 피트모스를 대체하는 원료로서 우드 칩의 가능성을 평가하였다. 이를 위해 열처리 우드 칩을 이용해 인공상토를 제조하여 상추의 생장에 대한 평가를 하였다. 피트모스와 열처리 우드 칩을 10:0, 7:3, 5:5, 3:7, 0:10 (w/w) 비율로 혼합하였으며, 그 결과 피트모스와 열처리 우드 칩의 발아율은 유의성이 없었으나, 근장은 피트모스와 열처리 우드 칩의 혼합 비 3:7(w/w)과 0:10(w/w)에서 피트모스 보다 약 1.6배, 1.7배 높은 것으로 확인 되었다. 뿐만 아니라, 초장에서는 열처리 우드 칩에서 피트모스 보다 약 1.2배 높은 성장률을 보였다. 따라서 열처리 우드 칩이 피트모스의 대체 원료로서 이용 가능할 것으로 판단된다.