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백서(白鼠)의 족삼리(足三里)에 시술한 비파엽약침(枇杷葉藥鍼)이 천식억제 및 면역조절효과에 미치는 영향
정주열 ( Ju Yel Jung ),임윤경 ( Yun Kyoung Yim ),이현 ( Hyun Lee ) 대한경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.4
To study the effects of EF(Eriobotryae Folium) herbal acupuncture on asthma, we injected EF-HAS into Jok-samni(ST36) of C57BL/6 mice Objective: The aim of this study was to investigate the effect of EF-HA(herbal acupuncture) at ST36 on ovalbumin-induced asthma in mice. Methods: C57BL/6 mice were sensitized and challenged with OVA(ovalbumin) for 12 weeks(once a week). Experimental groups were treated with concentrations(1%) of EF-HA at Jok-samni(ST36) for the later 8 weeks(3times/week). Result: 1. The weight and total cells of lung of the mice group treated with EF-HA decreased significantly compared with that of Control group. 2. Total Leukocytes and Eosinophils in BALF of the mice group treated with EF-HA decreased significantly compared with those of Control group. 3. The sticking of collagen on histological analysis of lung sections, the mice group treated with EF-HA decreased significantly compared with those of Control group. 4. The concentration of IgE, IL-4, IL-5 in BALF of the mice group treated with EF-HA decreased significantly compared with those of Control group. 5. The concentration of IL-4, IL-5, IL-13 in Serum of the mice group treated with EF-HA decreased significantly compared with those of Control group. 6. The number of Gr-1+/CD11b+, CD3-/CCR3+, CD4+, CD8+, CD3e+/CD69+ cells in the lungs of the mice group treated with EF-HA decreased significantly compared with those of Control group. 7. The cytokine`s manifestation of mRNA of the mice group treated with EF-HA with RT-PCR decreased significantly compared with that of Control group. Conclusion: We conclude that EF-HA is effective on OVA-induced asthma of C57BL/6 mouse.