발단 단계별로 분할한 생쥐 수정란에 Rat H-Y 항체 처리가 분할란의 발달에 미치는 영향

정장용,박희성,문승식,박성진 진주산업대학교 1993 論文集 Vol.32 No.-

This experiment was carried out to develop a new technique of identifying XX or XY-bearing bisected embryos prior to implantation by immunological method. H-Y antiserum prepared in inbred Wistar female rats by repeated immunization with spleen cells from males of the same strain. The reactivity of H-Y antibody was confirmed by culturing mouse embryos in the medium containing H-Y antiserum and complement obtained from the guinea pig. The optimal condition for the activity of H-Y antibody was also investigated by culturing embryos under the concentration or affected H-Y antibody and culture rate. The bisection rate of mouse embryo at 4-cell stage(64.6%) was significantly(P<0.05) low than 8-cell(76.0%), morula(81.2%) or blastocyst(81.3%). And not significantly(P<0.05) different from 4 to 8-cell and morula to blastocyst stage embryos. When the bisected embryos without any damage from microsurgery were cultured in vitro up to twin blastocyst or twin reformated blastocyst, the in vitro development of demi-embryos bisected at morula to blastocyst was 81.6 to 84.7%. However, the in vitro development of demi-embryos bisected at 4-cell(31.6%) to 8-cell(37.8%) stage was significantly(P<0.05) lower. The lysis-rate of bisected 4- to 8-cell and morula to blastocyst embryo was 42.7 and 58.6%. The morphology embryos of zona-free and intact embryos showed the embryos lysis rate of 48.5 and 42.7% respectively(P<0.05).

Rhizopus製劑 添加給與가 肉鷄의 血液像에 미치는 影響

鄭場龍,文勝式,尹東根 진주산업대학교 1984 論文集 Vol.22 No.-

This study was conducted to observe the effect of four Rizopus levels C(0%), T_1(0.04%), T_2(0.08%) and T_3(0.12%) on Hematological valuse in male and female broiler chicks at 56days of age The results obtained were summarized as follows: 1. The mean Erythrocyts count was 3.50±0.19×10exp(6)/㎣ in female and 3.70±0.48×10exp(6)/㎣ in male and all treatments was higher than that of control, and especially Rhizopus at level of 0.04% were the highest among all treatments. 2. The mean hemoglobin value was 8.45±0.49gm/100㎖ in female and 8.36±0.74gm/100㎖ in male and it counts at each level of addition were high in order of T_3(8.27±0.59gm/100㎖) C and T_2(8.41±0.47gm/100㎖) and T_1(8.70±0.24gm/10㎖)in female and C(7.76±0.47gm/100㎖), T_3(8.30±0.74gm/100㎖) T_2(8.56±0.44gm/100㎖) and T_1(8.88±0.50gm/100㎖) in male. 3. The hematocrit value was 29.83±1.34% in female and 30.25±2.20% in male, and it have not tendency on treatment Rhizopus at the levels of addition. 4. The mean MCV value was 84.35±5.19μ^3 in female and 83.22±12.62μ^3 in male. 5. The mean MCH was 23.87±1.57μ㎍ in female and 22.91±2.96μ㎍ in male. 6. The mean MCHC value was 28.35±1.75% in female and 27.79±3.14% in male. 7. The mean Leukocyte count was 8.87±0.58×10exp(3)/㎣ in female and 9.24±1.24×10exp(3)/㎣ in male. 8. The mean differential count of Neutrophils, Basophils, Lymphocytes and Monocytes was 23.00±1.87%, 1.75±1.30%, 1.75±0.83%, 72.00±2.12% and 1.5±0.5% in female and 22.50±2.29%, 2.5±1.66%, 1.00±0.71%, 71.75±0.83% and 2.25±1.48% in male.

Rat H-Y 抗體에 의한 생쥐 分割卵의 性 調節에 關한 硏究

鄭場龍,朴喜成,朴忠生 한국동물생명공학회(구 한국동물번식학회) 1991 Reproductive & developmental biology Vol.15 No.3

중·장기 비육우 경영에 있어서 생산성의 비교 분석

정장용,박화성,문승식,박성진 진주산업대학교 산업과학기술연구소 1994 산업과학기술연구소보 Vol.- No.1

우리나라의 비육우 형태를 중기 비육과 장기 비육으로 나누어 비교분석 하였을때 1일 노동 투하량에 있어서는 1992년 중·장기비육시 각각 0.40시간과 0.35시간으로 장기 비육시가 노동시간이 적게 소요되었고 농후사료의존도는 50∼6O%로 중기 보다 장기 비육시 1일사료 급여량이 적었다. 판매시 체중은 1992년 중기 비육시 495kg과 장기 비육시 499kg로 1988년 각각 448kg과 427kg에 비해 크게 증가되었으며, 1992년 일당 증체량은 중·장기 비육시 각각 0.93kg과 0.91kg로 나타나 1kg 이하 수준이었고, 1992년 사료효율 면에서는 중·장기 비육시 각각 0.12kg와 0.13kg로 장기 비육시 다소 높았다. 1인 사육가능두수는 중기 비육의 경우 1988년 13두에서 1992년 25두로 장기 비육의 경우 각각 15두에서 29두로 중·장기 비육 모두 약 2배로 증가 되었으나 규모의 확대를 통한 노동 생산성을 꾀하여야 한다. 생산비중 가장 큰 비중을 차지하는 가축비 및 사료비로서 비용합계의 70~80%로서 이중 송아지 가격의 적정화가 필요하다. 따라서 중 장기 비육간 비교분석 결과 장기 비육이 바람직하다고 하겠다. The purpose of the survey was to obtained data on production costs of livestock products which may be utilized to strive for stabiliation on livestock prices, and providing with regarded to the live stock industry. Compared with the labor hours and feed quantities of per day in 1992 year, long-term fattening of beef cattle(0.35 hour) decreased than mid-term fattening(0.40 hour). The marketing weight was increased in 1992 year(mid- : 495kg , long- . 499kg) than 1988 year(mid- ; 448kg : long ; 427kg). And daily gains of beef cattle in 1992 year was similar to that of mid- and long- term fattening. Livestock and feed cost were 70~80% at total production cost. And required stability of calf production cost. Long-term fattening should be better in beef cattle management.

肉鷄後期飼料의 給與時期가 鷄肉의 理化學的 成長에 미치는 影響

鄭場龍,朴成振,宋瑛敏 진주산업대학교 1985 論文集 Vol.23 No.-

本 試驗은 肉鷄後期飼料의 給與時期를 3週齡(A) 4週齡(B) 5週齡(C) 및 6週齡(D)時 달리했을 때 브로일러의 成長에 미치는 影響을 살펴보고자 1985年 5月 2日부터 同年 6月 13日(42日間)에 걸쳐 晋州農林專門大學 動物飼育場 育雛室에서 實施하여 다음과 같은 結果를 얻었다. 1. 增體量은 3주령시 給與區(A)가 2,272.15g로 가장 좋았으며 각 구간에 差異(p<0.01)가 인정되었다. 2. 飼料攝取量은 4주령시 給與區(B)가 5,256.31g로 타구에 비해 良好했으며 각 구간에 差異(p<0.01)가 있었고 飼料要求率도 각 구간에 차이가 있었다(p<0.01). 3. 産肉指數는 A구(330.70) B구(280.16) D구(267.02) 및 C구(263.00)로 각 구간에 差異가 인정되었다(p<0.01). 4. 屠體率은 A구(73.13) C구(71.98) B구(71.83) 및 D구(71.76) 順으로 나타났다. 5. 肉鷄 1首當 收入은 A구가 480,22으로 가장 좋았고 B구(364,29원), C구(304,71원) 및 D구(244,92원)의 順이다. This experiment was conducted to investigate the effect of feeding time of broiler finisher diets at 3 weeks(A), 4 weeks(B) , 5 weeks(C) and 6 weeks(D) on growth in broiler for 42 days from May 2, 1985 to June 13, the same year at brooder house, Chinju Agricultural and Forestry Junior Technical College. The results obtained were summarized as follws: 1. Body weight gain was the highest in the A plot(2,272.15g)and there were significant(P<0.01) differences between the all plots. 2. Feed intake was superior in the B plot(5,256.31g) to the other plots and there were significant (p<0.01) differences between the all plots, and feed conversion was significant (p<0.01) differences between the all plots. 3. Point spread was 330.70(A), 280.16(B), 267.02(D) and 263.00(C) in the order and there were significant(p<0.01) differences between the all plots. 4. Dressing percentages was 73.13(A), 71.98(C), 71.83(B) and 71.76(D) in the order. 5. In economical analysis of broiler production per bird the income was the highest in the A plot in the order of 480.22(A), 364.29(B), 304.71(C) and 244.92 won(D).

소 난포란의 체외성숙도가 수정 및 체외발달에 미치는 영향

정장용,박희성,박성진,문승식,정현승 진주산업대학교 농업기술연구소 1995 農業技術硏究所報 Vol.8 No.-

본 연구소는 소 난포란을 이용하여 체외수정시에 체외성숙 시간이 체외수정 및 체외발달에 미치는 영향을 조사하고자 실험을 수행하였다. 도살장에서 도살된 한우의 난소를 채취하여 실험실로 2시간 이내에 운반하여 난소를 깨끗이 세척한 후 18 gauge needle로 난포의 크기별로 난포란을 회수하여 10% FCS가 첨가된 TCM-199 배양액에 FSH(35 ㎍/ml), LH(10㎍/ml), estradiol-17β(1㎍/ml)가 첨가된 체외성숙용 배양액에서 20-28시간 동안 체외성숙을 유도하였다. 체외성숙란을 한우 동결정액을 이용하여 체외수정을 시킨 후 난관상피세포에서 공배양을 실시하여 수정란의 체외발달을 유도하였으며, 난포란의 등급별 회수율과 체외성숙시간에 따른 수정율 및 체외발달율을 조사한 결과는 다음과 같다. 난소에서 난포의 크기에 따른 Grade I의 회수율은 소, 중 및 대난포에서 각각 31.3, 27.8 및 3.7%였으며, 48개의 난소로 부터 모두 398개의 미성숙 난자를 회수하여 난소당 8.3개의 미성숙 난자를 회수하였다. 등급별로는 2.3, 2.5, 2.3 및 1.2%의 회수율을 보였다. 체외수정란의 배반포로의 발달율은 24시간 성숙시켰을 때 27.2%로서 20(11.6%) 및 28(9.0%)시간 성숙시켰을 때 보다 유의적(P<0.05)으로 높았다. 그러나 22, 24 및 26시간 성숙시켰을 때는 성숙시간에 따른 발달율은 유의적(P<0.05)인 차이가 없었다. The ovaries of Korean native cow or heifers were obtained from an abattoir and kept on 28-30℃ and transported to laboratory within 2 hrs. The follicular oocytes were collected from small, medium and large follicles, and classified into 3 grades by the morphology of cumulus cells attached and homogenous cytoplasm. The oocytes were matured in vitro for 24 hrs. in TCM-199 supplemented with 35㎍/ml FSH, 10㎍/ml LH, 1㎍/ml estradiol-17β and granulosa cells at 39℃ under 5% CO_2 in air. They were fertilized in vitro by freezing sperm treated with heparin for 24 hrs., and then the zygotes were co-cultured in vitro with bovine oviductal epithelial cells for 7 to 9 days. The follicular oocytes recovered were classified into 41.7% as gradae Ⅰ, 51.5% as grade Ⅱ and 6.8% as grade Ⅲ. The number of oocytes recovered per ovary was averaged 8.3 and they were classified into 2.3 as grade Ⅰ, 2.5 as grade Ⅱ and 2.3 as grade Ⅲ. The cleavage rates of the embryos obtained from 73.3, 78.4, 80.4, 77.3 and 72.7% in the groups of maturation age of 20, 22, 24, 26 and 28 hours respectively. The in vitro culture of bovine embryos with maturation age from 22-26 hours improved significantly(P<0.05) the subsequent development to transferable embryos(20.4-25.0%), compared with 20 hours(11.6%) or 28 hours(9.0%).

卵用鷄의 初産日齡이 産卵能力과 卵重에 미치는 影響

鄭場龍,文勝式,朴成振 진주산업대학교 1986 論文集 Vol.24 No.-

본 試驗은 初産日齡이 産卵能力과 卵重에 미치는 영향을 살펴보고자 1986년 2월 1일부터 11월 30일까지 晋州農林專門大學 부속 動物飼育場에서 실시하였다. 初産日齡이 166~168일의 닭들이 165일 이전 또는 169일 이후의 개체들보다 産卵能力이 우수하였고 平均卵重은 初産日齡間에 차이가 없이 비슷하였다. 90%이상 産卵지속은 초산일령이 166~168일의 닭들이 26주령시부터 48주령시까지 지속되어 가장 우수하였다. For the purpose of identifing the reationship between the first egg day and other economic traits; egg production and egg weight, this experiment was divided 160 birds(DEKLB-Warren Sex-Sal-Link) into four first egg day groups and observed egg number and gee weight every day during 10 months form 1st February, 1986 to 30st November, the same year at Animal farm, Chin Ju Agricultural and Forestry Junior Technical College. Bird showing 166-168 day in first egg day layed the most eggs among four groups and they produced similar to average egg weight between the four groups. In egg production above 90%, 166-168 day group showed the best record as a 26-48 week.

한우 체외수정란의 동결보존 및 핵이식에 관한 연구 : Ⅰ. 계절 및 배양조건이 한우 체외수정란의 생산에 미치는 영향 Ⅰ. Effects of season and culture condition on production of bovine in vitro fertilization embryos

정장용,박희성,桑潤滋,離素娟 진주산업대학교 농업기술연구소 1998 農業技術硏究所報 Vol.11 No.-

본 연구는 도살장에서 도살되는 한우의 난소를 적출하여 난소로부터 미성숙 난포란을 회수하는데 있어서 계절별 난포란의 quality과 수정율의 차이점에 대한 규명과 체외성숙-체외수정한 난자를 체외배양기법을 확립하고자 본 실험을 수행하였다. 도살된 한우의 난소로 부터 난포의 크기별로 난포란을 회수하여 체외성숙을 유도하였다. 체외성숙란을 정소상체 미부정자를 이용하여 체외수정을 시킨 후 난관상피세포에서 공배양을 실시하여 수정란의 체외발달을 유도하였으며, 난포란의 계절별 회수율, 체외수정율 및 배양체계에 따른 체외발달율을 조사한 결과는 다음과 같다. 봄(426개), 여름(433개), 가을(419개) 및 겨울(428개) 등 계절별로 분류하여 모두 1,706개의 난소로부터 14,674개의 난포란을 채취하였다. 계절에 따른 1등급 회수율은 22.2(봄), 6.5(여름, 20.6(가을) 및 18.1%(겨울)로써 이들간에 유의적(P<0.05)인 차이는 없었으나 겨울철에 회수한 난자들이 다소 낮게 나타났다. 회수된 2등급 난자의 비율도 봄, 여름, 가을 및 겨울이 각각 43.7, 41.6, 44.1 및 35.2% 로써 계절에 따른 유의적(P<0.05)인 차이가 없었다. 봄, 여름, 가을 및 겨울 등 계절별 체외수정율은 각각 76.0, 80.6, 75.7 및 72.1% 로써 계절에 따른 수정율은 유의적(P<0.05)인 차이가 없었으며, 배반포기 단계까지 발달한 체외수정란은 TCM-199 및 Hams-F10 배양액에 10% FCS와 난관상피세포를 공배양을 실시하였을때는 각각 23.3 및 21.7%의 발달율을 보였으나, TCM-199 배양액에 10%FCS만을 첨가하였을 때는 전혀 발달이 되지 않았다(P<0.05). 본 연구에서는 계절별 회수율의 차이는 많은 요인이 있을 수 있겠으나 주로 계절별 사양관리의 차이 및 계절에 따른 조사료의 공급수준에 의한 영양수준의 차이 등에서 기인되는 것으로 생각된다. 체외수정란의 배반포기로의 발달을 위한 배양조건은 TCM-199 또는 Hams-F10배양액에 10% FCS를 첨가한 배양액으로 난관상피세포와 공배양을 실시하는 것이 효과적인 방법이라고 생각된다. The present study was carried out to determin the seasonal effects of oocyte collection and culture condition of Korean native cows and in vitro developmental rate of 1VF bovine embryos. The follicular oocytes were collected from small, medium and large follicles, and classified into 4 grades by the morphology of cumulus cells attached and homogenous cytoplasm. They were fertilized in vitro by epididymis spermatozoa treated with heparin for 24 hrs., and then the zygotes were co-cultured in vitro with bovine oviductal epithelial cells for 7 to 9 days. The results obtained were summarized as follows : The follicular oocytes recovered were classified into grade I 22.2% as spring, 26.5% as summer, 20.6% as autumn and 18.1% as winter. The cleavage rates of the embryos obtained from 76.0(spring), 80.6(summer), 75.5(autumn) and 72.1(winter) respectively(P<0.05). The developmental rates(23.3 and 21.6%)of embryos cultured in TCM 199+10% FCS-OCEM and Harms-F10+10%FCS+OECM up to blastocyst stage were higher(P<0.05) than the TCM-199+10%FCS culture condition(P<0.05)

烏骨鷄의 特性에 關한 硏究 : 第Ⅱ報 烏骨鷄精液의 液狀保存과 性狀에 관한 硏究 Ⅱ. Studies on the Characteristic and liquid semen of korean Native ogolgye semen

鄭場龍,朴成振,宋瑛敏 진주산업대학교 1981 論文集 Vol.19 No.-

This experiment was carried out to develop dilutor for the usage of liquid semen and characteristic of Korean native Ogolgye semen. The results obtained in this experiment was summarized as follows, 1. The characteristic of Ogolgye semen used in this experiment showed of 5 month age 0.32ml(0.13∼0.46ml) average semen volume, 14.9×10 exp(8)) sperm number per ml, 18% (15∼21%) sperm abnormality, 82 live sperm index, 7.5pH, 96.57μ spermatozoa size, 12.44μ head size and 0.98μ head wide, and average of 6 month age 0.39ml(0.32∼0.45ml) semen volume. 17.8×10 exp (8)(12.2∼21.1×10 exp (8)) sperm number per ml. 14%(11∼20%) sperm abnormality, 83 live sperm index, 7.5pH, 100.05μ spermatozoa size, 12.65μ head size and 1.00μ head wide. 2. Diluted semen with 0.9Nacl and 5% glucose solution showed high sperm motility same whole semen just after dilution. 3. Ogolgye semen diluted in 0.9Nacl 5% glucose and mixture solution(0.9Nacl 1: 5% glucose 1) and storaged for 6hr showed no difference in sperm motility index between storage temperatures at 5 and 25℃. 4. Diluted semen with the mixture of 0.9% Nacl 5% glucose with same volume showed high sperm motility just after dilution. 5. It is known that Ogolgye whole semen has comparatively strong resistance against cold shock at 5℃ for min. 6. The semen diluted with 5% glucose solution showed better sperm motility than the semen diluted with 0.9% Nacl solution. 7. Semen extended with dilutor containing 0.8mg hostacillin and lmg streptomycin per ml of dilutor showed higher sperm motility index than that without antibiotics during storage.

돼지의 분만유기에 관한 연구

정장용,남연우 진주산업대학교 농업기술연구소 1997 農業技術硏究所報 Vol.10 No.-

본 연구는 분만을 유기하기위하여 분만유도제인 tiaprost 0.3∼0.6mg을 임신 111일째 투여한후 20시간째 carazol과 oxytocin의 수준을 달리하여 분만유기를 시도한 실험1에서 144두중 133두가 실험2에서 140두 중 135두가 분만유기되어 92%와 96%의 높은 성적을 얻었으며 carazol 수준 1.5mg과 3.0mg을 처리했을 때 분만유기 및 분만완료까지의 시간도 단축되었으나 oxytocin의 수준간에는 차이를 나타내지 않았다. The present study was carried out to establish a practice for farrowing induction using tiaprost, carazol and oxytocin through conception stage in sows. After administration of 0.3-0.6mg tiaprost of farrowing induction in conception 111 days, Total 133 heads out of 144 heads in experiment 1 and Total 135 heads out of 140 heads in experiment 2 which were the treated difference interval carazol and oxytocin to farrowing in 20th were farrowed and obtained high result of 92% and 96% and treatment of carazol interval 1.5mg and 3.0mg, time of farrowing induction and farrowing completion were shorten but oxytocin interval did not show difference.