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        참돔이리도바이러스 감염 돌돔에서 임상적 변화와 viral genome copy number 간의 상관관계

        신동준 ( Dong Jun Shin ),정이설 ( Yi Seol Jeong ),김민재 ( Min Jae Kim ),김국현 ( Guk Hyun Kim ),김광일 ( Kwang Il Kim ) 한국어병학회 2023 한국어병학회지 Vol.36 No.2

        In this study, the correlation between clinical changes and RSIV genome copy number was investigated to determine the quantitative criteria for the characteristics of RSIV infection. The rock bream (Oplegnathus fasciatus) was intraperitoneally injected with three different doses (1.0×10<sup>1</sup>, 1.0×10<sup>3</sup> and 1.0×10<sup>5</sup> viral genome copies/fish) as low, medium, and high doses, respectively. The clinical signs (spleen enlargement, death) observation and real-time PCR were conducted at 5, 10 and 14 days post-injection. During the experiment, spleen index as a quantitative indicator for spleen enlargement was continuously increased in the medium- (up to 2.26) and high-dose (up to 4.99) challenge groups, respectively. Notably, when the spleen index was over 1.5, 2.0, 2.5 and 3.0, a positive correlation was revealed with average viral genome copy numbers of 2.51, 3.37, 4.97 and 5.43×10<sup>7</sup> viral genome copies/mg, respectively. Moreover, the threshold of spleen index over 1.5 was 1.0×10<sup>6</sup> viral genome copies/mg, while the thresholds of spleen index over 2.0 and dead was 2.51×10<sup>7</sup> viral genome copies/mg and the thresholds of spleen index over 2.5 and 3 was 3.98×10<sup>7</sup> viral genome copies/mg. These findings suggest the possibility of quantitatively analyzing the characteristics and development process of RSIV infection.

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