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Polymerase chain reaction으로 설사 영유아 대변 검체에서 adenovirus type 40, 41의 분리 동정
박혜경,서주영,정영해,김경희 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-
Adenovirus is the second leading cause of acute gastroenteritis. As the children with diarrhea by acute adenoviral gastroentertis usually require hospitalization, rapid diagnosis is necessary for proper management. Adenovirus infection can be diagnosed conventionally by the direct electron microscopic observation of the virus particle, isolation of the virus by cell culture, or enzyme immuno assay. Recently, polymerase chain reaction is applied for the detection of adenovirus. In this study, we investigated stools collected from 100 children hospitalized for diarrhea in Ewha womans University Hospital from the September of 1995 to the July of 1996 for adenovirus by polymerase chain reaction. Two oligonucleotide primers, desiginated as hexAA1885 and hexAA1913, were used for the detection of enteric adenovirus type 40 an 41 in PCR. After amplification pf 35 cycles, 300 bp fragments were detected on 1.8% Sea Kem GT agar gel in 14 samples. The stool samples were further analyzed by electron microscopy and immunoblotting. In electron microscopy, icosahedral particles of about 75nm were observed in a^7 of the 14 samples. The 14 samples were collected from children whose mean age was 1.4 years and the sex ratio was 10/4(M/F). The seasonal variation showed acute gastroenteritis by adenovirus infection was frequent in March to May and in September to October in Korea.