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- 저자 : 정영필(Young-Pil Jung) (검색결과 4 건)
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ICR 마우스를 이용한 이중탕 및 발효이중탕의 급성독성 연구
정영필 ( Young Pil Jung ),황윤환 ( Yun Hwan Hwang ),이지혜 ( Ji Hye Lee ),임남희 ( Nam Hui Yim ),조원경 ( Won Kyung Cho ),마진열 ( Jin Yeul Ma ) 대한본초학회 2012 大韓本草學會誌 Vol.27 No.3
Objectives: This study was carried out to investigate the acute toxicity and safety of Leejung-tang(Lizhong-tang) and fermented Leejung-tang(Lizhong-tang) extract. Methods: To evaluate their acute toxicity and safety, 0(control group), 1250, 2500 and 5000 ㎎/㎏of Leejung-tang(Lizhong-tang) and fermented Leejung-tang (Lizhong-tang) extracts were orally administered to 35 male and 35 female ICR mice. After a single administration, we observed survival rates, general toxicity, changes of body weight, and autopsy. Results: Compared with the control group, we could not find any toxic alteration in any of treated groups (1250, 2500 and 5000 ㎎/㎏). Conclusions: LD50 of Leejung-tang(Lizhong-tang) and fermented Leejung-tang (Lizhong-tang) were over 5000㎎/㎏and it is very safe to ICR mice.
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ICR 마우스를 이용한 발효삼출건비탕의 단회투여 독성에 대한 연구
정영필 ( Young Pil Jung ),임남희 ( Nam Hui Yim ),김애영 ( Ae Yung Kim ),황윤환 ( Youn Hwan Hwang ),박화용 ( Hwa Yong Park ),마진열 ( Jin Yeul Ma ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.2
Objectives: Samchulgeonbi-tang (shenzhujianpi-tang) has been prescribed as one of traditional herbal medicine for treatment of stomach diseases since ancient time in Korea. Samchulgeonbi-tang extract was fermented by Lactobacillus spp. for improving the effect. However, the toxicity and safety of fermented Samchulgeonbi-tang (FS) extract were not confirmed. Therefore, this study was performed to evaluate the acute toxicity and safety of FS extract. Methods: To evaluate the acute toxicity and safety of FS extract, several doses of FS extract, 0, 500, 1000 and 2000 ㎎/㎏, were orally administered to 20 male and 20 female ICR mice, respectively. After treatment with FS extract, we observed mortality, general toxicity, behavior and change of body weight for the 14 days. After 14 days of oral administration, all mice were sacrificed and hematological parameters were analyzed from blood serum. Results : In present study, the toxic signs such as mortality or abnormal behaviors by FS extract were not observed. There are no significant differences between FS-treated group and control group in body weight, organ weights, and hematological parameters. Conclusions : The remarkable adverse effects by FS extract were not observed in ICR mice. Also, any death was not occurred at all treated FS doses, 500, 1000 and 2000 ㎎/㎏. Therefore, the approximate lethal dose (ALD) of FS extract may be more than 2000 ㎎/㎏.
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인삼토양으로부터 옥신 생성 식물생장촉진세균의 분리 및 특성
박해성(Hae-Sung Bak),정영필(Young-Pil Jung),윤민호(Min-Ho Yoon) 충남대학교 농업과학연구소 2010 농업과학연구 Vol.37 No.3
An auxin-producing bacterium (AMT-54) was isolated from ginseng cultivating soil of Geumsan area. The isolate AMT-54 was confirmed to produce indole-3-acetic acid (IAA) which is one of auxin hormone by TLC analysis. When the concentration of IAA was assessed by performing HPLC quantitative analysis, the maximal 457ppm of IAA was detected from the culture filtrate after culturing in R2A broth containing 0.1% tryptophan for 24h at 35℃. The molecular weight of the main peak obtained by LC-mass analysis was correspondent well to 175, that of IAA. The strain AMT-54 was identified as a novel species belongs to Klebsiella mobilis by a chemotaxanomic and phylogenetic analysis. To investigate the growth promoting effect of crop, when the culture broth of K. mobilis AMT-54 was infected onto seed pot of mung bean, the adventitious root induction and root growth of mung bean were 3.3times higher than control.
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혼용자재 특성이 양송이 폐상배지를 이용한 퇴비제조에 미치는 영향
경기천(Ki-Cheon Kyung),이희덕(Hee-duk Lee),정영필(Young-Pil Jung),장갑열(Kab-Yeul Jang),윤민호(Min-Ho Yoon) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.3
This study was conducted to select organic materials (OM) and nitrogen sources in composting of waste mushroom bed from Agaricus bisporus. We examined physio-chemical properties of the organic materials and the mixture ratio for preparing the wasted mushroom bed (M) compost. The carbon content of sawdust was higher than those of rice straw (R) as OM source and the nitrogen content was high in the order of fowl manure (F)>> pig manure (P)> cow manure (C). The compost was prepared to maintain the criteria of above 25% organic matter and then the change of their ingredients was estimated during the process of fermentation. The temperature of waste mushroom bed+pig manure+rice straw (MRP) treatment was varied fast throughout fermentation, on the other hand the temperature of waste mushroom bed+pig manure+sawdust (MSP) treatment was steadily elevated to the middle of composting. The pH of the compost was somewhat high to pH 8.5~9.0 at the early stage, but decreased to 7.5 at the end stage of composting. The content of OM after fermentation was decreased to the level of 19~21% in rice straw, but the sawdust treatment maintained 25~27% organic matter. The waste mushroom bed+fowl manure+rice straw (MRF) treatment, which contains 26.2% organic matter and 0.68% nitrogen, was the highest among them. The volume of compost was reduced to 50% by using rice straw as organic matter, but reduced to 30% by using the sawdust. The contents of heavy metal in the compost were suitable within the legal criteria. The number of microorganisms were higher in the rice straw than those in the sawdust. It was high in the order of fowl manure> pig manure> cow manure. The major groups consisted of aerobic bacteria, gram negative bacteria and Bacillus sp. and their populations after fermentation were increased to 1×10¹~1×10² cfu g<SUP>-1</SUP> rather than those before fermentation. Therefore we concluded that the waste mushroom bed+fowl manure+sawdust (MSF 3:9:1 v/v/v) treatment was suitable combination for high organic matter and nitrogen source, and the periods of composting were 50~60 days.
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