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사람 말초혈액 단핵세포에서 녹두 렉틴의 사이토카인 생성효과
전경희,안몽기,정수민,최경민,이승호,정시련,Jeune, Kyung-Hee,An, Mong-Gi,Jung, Su-Min,Choi, Kyung-Min,Lee, Seung-Ho,Chung, See-Ryun 한국생약학회 1999 생약학회지 Vol.30 No.4
New lectins have been isolated and purified from mung bean (Phaseolus radiatus) through physiological saline extraction, ammonium sulfate salt fractionation and column chromatographies. Ion exchanger were eluted by linear salt gradient and then further purified through gel filtration. Thus obtained lectin named as MBL. The gene expressions of 5 cytokines (IL-1, IL-2, IL-6, $TNF-{\aphpa}$ and $IFN-{\gamma}$) from human peripheral blood mononuclear cells (PBMC) stimulated with MBL were investigated by using reverse transcription polymerase chain reaction (RT-PCR). PBMC ($1{\times}106$ cells/ml) isolated from healthy volunteers were stimulated with lectins (4 mg/ml) for various time intervals (1 to 96 hrs). After each of the various stimulated times, total RNA was isolated and assessed for different cytokines mRNA by RT-PCR. The mRNA encoding IL-1, IL-2 were detected continuously from 1 to 20 hrs, and IL-6 was detected up to 24 hrs. But the mRNA encoding $IFN-{\gamma}$ and $TNF-{\alpha}$ were detected to 8 hours only and showed short time response compared with other cytokines. The significant expression of all cytokines mRNA were observed at 4 hrs. These results suggested that MBL, as inducer of cytokines could elicit detectable cytokine mRNA from PBMC.
장수풍뎅이(Allomyrina dichotoma) 렉틴의 면역기능 증강효과
전경희,정미연,최수정,이종욱,박원학,조세훈,이승호,정시련,Jeune, Kyung-Hee,Jung, Mi-Yeun,Choi, Soo-Jeong,Lee, Jong-Wook,Park, Won-Hark,Cho, Se-Hoon,Lee, Seung-Ho,Chung, See-Ryun 한국생약학회 2001 생약학회지 Vol.32 No.1
A lectin was purified from Allomyrina dichotoma (ADL) by physiological saline extraction, ammonium sulfate fractionation, anion exchange column chromatography on DEAE Sephadex A-50 and gel filtration column chromatography on Sephadex G-200. Several biochemical properties of ADL were characterized as follows: ADL from gel filtration column chromatography showed single band on SDS-PAGE. ADL agglutinated the erythrocytes of rabbit and human A, B, O, AB. Agglutinability was relatively stable at basic pH, and was stable at temperature below $40^{\circ}C$. Agglutinability was not affected by metal ions and EDTA. This lectin was proved to be a glycoprotein which contains 0.47% of sugars. The molecular weight of ADL was estimated to be 97,000 dalton by SDS-PAGE. By amino acid analysis, ADL exhibited high amounts of aspartic acid. The lectin's immunomodulating effect was measured as cytokine production. The productions of 5 cytokines $(IL-1{\alpha},\;IL-2,\;IL-6,\;IFN{\gamma}\;and\;TNF{\alpha})$ from peripheral blood mononuclear cells were measured by ELISA. The lectin induced the highest secretion of IL-2 at 8 hr, $TNF{\alpha}$ at 4 hr, and $IFN{\gamma}$ at 24hr, respectively. These results suggest that ADL can elicit the production of detectable cytokines from PBMC.
대극과식물 등대풀로부터 분리한 가수분해형 탄닌의 tyrosinase활성억제효과
김진준(Jin Joon Kim),이주상(Joo Sang Lee),김소영(So Young Kim),김정아(Jeong Ah Kim),정시련(See Ryun Jung),장태수(Tae Soo Jang),이승호(Seung Ho Lee) 대한약학회 2001 약학회지 Vol.45 No.2
Nineteen hydrolyable tannins isolated from Euphorbia helioscopia(Euphorbiaceae) were tested for the inhibitory effect on mushroom tyrosinase activity in vitro. Inhibitory effect of gallotannin group exhibited more potent than that of phenocarboxylic acid and ellagitannin groups against the enzyme activity. The inhibitory activity by pentagalloyl glucose on mushroom tyrosinase was more potent(IC50, 4.9uM) than that of kojic acid(IC50, 8.7uM)
사람 말초혈액 단핵세포에서 녹두 렉틴의 사이토카인 생성효과
전경희,안몽기,정수민,최경민,이승호,정시련 영남대학교 약품개발연구소 2000 영남대학교 약품개발연구소 연구업적집 Vol.10 No.-
New lectins have been isolated and purified from mung bean(Phaseolus radiatus) through physiological saline extraction, ammonium sulfate salt fractionation and column chro-matographies. Ion exchanger were eluted by linear salt gradient and then further purified through gel filtration. Thus obtained lectin named as MBL. The gene expressions of 5 cytokines(IL-1, IL-2, IL-6, TNF-α and IFN-?) from human peripheral blood mononuclear cells(PBMC) stimulated with MBL were investigated by using reverse transcription polymerase chain reaction(RT-PCR). PBMC(1×106cells/㎖) isolated from healthy volunteers were stimulated with lectins(4㎎/㎖) for various time intervals(1 to 96 hrs). After each of the various stimulated times, total RNA was isolated and assessed for different cytokines mRNA by RT-PCR. The mRNA encoding IL-1, IL-2 were detected continuously from 1 to 20 hrs, and IL-6 was detected up to 24 hrs. But the mRNA encoding IFN-r and TNF-α were detected to 8 hours only and showed short time response com-pared with other cytokines. The significant expression of all cytokines mRNA were observed at 4 hrs. These results suggested that MBL, as inducer of cytokines could elicit detectable cytokine mRNA from PBMC.