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제주의 양식 넙치(Paralichthys olivaceus)를 대상으로 한여윔증 모니터링(2010-2013)
김승민 ( Sungmin Kim ),전려진 ( Lyujin Jun ),박명애 ( Myoungae Park ),정승희 ( Sunghee Jung ),정현도 ( Hyundo Jeong ),정준범 ( Joonbum Jeong ) 한국수산과학회(구 한국수산학회) 2015 한국수산과학회지 Vol.48 No.5
In this study aiming at the cultured olive flounders in Jeju island of Korea, has performed a total of 216 cases of pathogen tests for the olive flounders which seemingly had the symptom of emaciation in 24 farms on the suspicion of its occurrence from June 2010 to December 2013 and intended to get the basic information about the pathogen. According to the survey results of the emaciation infection status of 24 farms in Jeju over the period of this survey, it was confirmed that 18 (75%) of 24 farms in Jeju are positive in the emaciation infection. Among them, as for the rate of infection per year, it was observed that they are 38% in 2010, 48% in 2011, 50% in 2012 and 60% in 2013, and over the period of this survey the infection rate in accordance with the temperature of water has observed a variety of incidence rates from the summer to the winter season. In addition, according to the results of emaciation infection status for each size, the equivalent showed a detection rate, 54% in 11-20 cm, 43.9% in 21-30 cm and 25.4% in over 31 cm. This result accounts for an important portion among the diseases of farmed olive flounders in Jeju, is considered to be one of the diseases which cause troubles in the farms for olive flounders on land and it is thought that it can be utilized as basic data in order to estimate emaciation which may occur in the similar size of the cultured olive flounders in Jeju island.
조피볼락(Sebastes schlegeli) 선충(Nematode: Philometridae)에 대한분자생물학적 동정 및 PCR 검출법 개발
서한길 ( Hangill Seo ),서정수 ( Jungsoo Seo ),류민경 ( Minkyung Ryu ),이은혜 ( Eunhye Lee ),정승희 ( Sunghee Jung ),한현자 ( Hyunja Han ) 한국수산과학회(구 한국수산학회) 2015 한국수산과학회지 Vol.48 No.5
Nematode infection in the epithelial tissue of cultured rockfish Sebastes schlegeli was first reported in 2012. Since then, nematode infections have caused serious economic losses in rockfish aquaculture on the west coast of Korea. Taxonomic and life cycle information for this parasite are currently unknown. In this study, 18S rRNA and cytochrome c oxidase subunit I (COI) genes were used for molecular identification and polymerase chain reaction (PCR) to detect the invisible stages of this parasite. Nucleotide sequences of the 18S rRNA of the rockfish nematode showed 98% identity with that of Philometra morii. Therefore, this rockfish nematode was classified to the Philometridae family. However, we could not identify it to genus level using 18S rRNA. Its COI nucleotide sequences shared 85% and 82% identities with those of Bursaphelenchus sinensis and Philometra overstreeti, respectively. In addition, two gene-specific primer sets were designed based on the 18S rRNA gene to detect the intermediate host and nematode larvae. These primers were specific to this rockfish nematode without cross-reacting to other pathogens. The detection limit of the PCR assay using these primers was 1,000 copies of nematoda plasmid DNA. Therefore, the PCR assay described here is suitable for the detection of nematode DNA within rockfish. In addition, this PCR assay could be used to detect nematode larvae and the intermediate host.