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형질전환 토마토에서 Cytosine Methylation에 의한 유전자발현 억제
정서희,민성란,이수영,박지영,정화지,전재홍,유장렬,정원중,Jung, Seo-Hee,Min, Sung-Ran,Lee, Soo-Young,Park, Ji-Young,Davarpanah, S Javad,Chung, Hwa-Jee,Jeon, Jae-Heung,Liu, Jang-Ryol,Jeong, Won-Joong 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.4
토마토 형질전환체에서 NPTII 및 TPSP 유전자의 발현양상을 조사하였다. 4개 line 토마토 형질전환체가 선발배지에서 kanamycin에 저항성을 보이며 생산되었다. 그러나, 1번 line과 11번 line의 후대에서 NPTII 유전자의 발현이 억제되었으며, Kanamycin 저항성을 보이지 못했다. Southern 분석 결과, 1번 11번 line은 여러 copy의 외래 유전자를 가지고 있었으며, 2번, 10번 line은 1-2 copy의 외래유전자를 가지고 있었다. 형질전환 line 11번은 methylation sensitive 제한효소처리 후 DNA methylation 분석 결과, TPSP coding부위 및 도입유전자의 발현을 조절하는 CaMV35S 프로모터 주위에서 CpG methylation이 축적되었음이 확인되었다. 따라서 여러 copy의 외래유전자를 가진 토마토 형질전환 line 11번에서 NPTII 유전자 및 TPSP유전자의 발현이 억제된 것은 transcriptional gene silencing 기작에 의한 것으로 추정되었다. Transgene expression was analyzed in tomato plants. Four lines of neomycin phosphotransferase II gene (NPTII) and the trehalose biosynthetic fusion gene (TPSP) transformed $T_0$ plants showed kanamycin resistance on selection medium. However, the analysis of phenotype (kanamycin resistance) and mRNA expression in $T_1$ plants indicated that the expression of the NPTII and TPSP transgenes was down-regulated to an undetectable level in two independent lines 1 and 11. Southern analysis demonstrated that the lines 1 and 11 had multicopies of the transgenes, whereas the typical transgenic lines 2 and 10 had 1 or 2 copies. DNA methylation analysis using methylation sensitive enzyme detected accumulated CpG DNA methylation on TPSP coding region and CaMV35S promoter region in the line 11, but not the typical transgenic line 2. These results suggest that multicopy transgene in plants is attributed to down-regulation of the transgene expression via transcriptional gene silencing.
정서희 ( Jeong Seo-hee ),김주용 ( Kim Ju-yong ),김광희 ( Kim Gwang-hee ) 한국건축시공학회 2023 한국건축시공학회 학술발표대회 논문집 Vol.23 No.1
The purpose of this study is to derive the cause of the quantity difference and present the considerations when take-off rebar quantity based on BIM model by comparing the quantity of rebar based on BIM model with 2D drawing. This research was limited to take-off the quantity of rebars in the building frame work, and after take-off the quantity of rebars by 3D modeling the 2D drawing of the target building with Revit, the quantity difference was compared with 2D-based software. Therefore, when take-off the quantity of rebars based on the BIM model, instead of take-off the existing 2D-based quantity premium proportion, according to general structural consider development length, lap splice length, covering thickness, reinforcing bars and spacing. In the future, this study is expected to contribute to improving the accuracy of BIM-based frame construction quantity take-off.
김희주 ( Kim Hee-ju ),정서희 ( Jeong Seo-hee ),박민영 ( Park Min-young ),이영도 ( Lee Young-do ),김광희 ( Kim Gwang-hee ) 한국건축시공학회 2022 한국건축시공학회 학술발표대회 논문집 Vol.22 No.1
In order to achieve structural performance such as rebar clear spacing in the reinforced concrete structure construction, coupler splicing joints are becoming common. To confirm the performance of rebar splicing joints, quality verification is being carried out through coupler specimen tests. However, it can be said that the required performance is reached only when the actual construction in the field is constructed under the same conditions as the coupler splicing joint specimen. Therefore, the problems and solutions of the coupler splicing joint construction of the actual field was investigated in this study.