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온실재배 오이의 생육단계별 목화진딧물의 주내 분포 특성에 기초한 축차표본조사법
정부근,송정흡,이흥수,최병렬,Chung, Bu-Keun,Song, Jeong-Heub,Lee, Heung-Su,Choi, Byeong-Ryul 한국응용곤충학회 2015 한국응용곤충학회지 Vol.54 No.4
This study describes the development of a method for monitoring Aphis gossypii in greenhouse cucumber fields that was used during 2013 and 2014. The dispersion pattern of A. gossypii was determined by commonly used methods: Taylor's power law (TPL) and Iwao's patchiness regression (IPR). The sample unit was determined by linear regression analysis between mean density of sample unit versus whole plant. The optimum sample unit for different plant growth stages was two leaves (median and the lowest + 1 leaf) when the total number of leaves was less than nine, and three leaves (4th, 7th from canopy, and the lowest +1 leaf) when the total number of leaves was greater than nine. A. gossypii showed an aggregated distribution pattern, as the slopes of both TPL and IPR lines were greater than 1. TPL provided a better description of the mean-variance relationship than did IPR. The slopes and intercepts of TPL and IPR from leaf samples did not differ between the surveyed years. Fixed precision levels (D) for a sequential sampling plan were developed using Green's and Kuno's equations based on the number of aphid in a leaf sample. Green's method was more efficient than Kuno's to stop sampling. The number of samples needed to estimate the density of A. gossypii increased at higher D levels and lower mean densities. The cumulative number of aphids needed to stop sampling increased at higher D levels and with fewer plants sampled. Thus to estimate 10 aphids per leaf, 13 plants needed to be sampled, and the cumulative number of aphids to stop sampling was 131.
7-Ethoxy precocene II 항유약호르몬유사물에 대한 몇가지 협력제의 Oncopeltus fasciatus Dallas에 있어서 협력작용
정부근,William S. BOWERS 한국응용곤충학회 1996 한국응용곤충학회지 Vol.35 No.3
몇가지 협력제의 7-ethoxy precocene II와 협력작용을 통한 활성증가와 작용기작을 구명하기 위하여 mikweed bug, Oncopeltus fasciatus 2령충에 항유약호르몬유사물과 협력제가 처리된 petri dish에 접종하여 생리활성 정도를 측정하였다. 얻어진 결과는 다음과 같다. 7-Ehtoxy precocene II의 반수활성농도는 $1.18\mu$g/$\textrm{cm}^2$이었다. 7-Ethoxy precocene II와 다른 산화저해제인 RO20-9747의 협력비가 14.05로 가장 높았고 그들의 반수활성농도는 $0.084\mu$$g/\textrm{cm}^2$이었다. 7-Ethoxy precocene II와 다른 협력제 1종, 2종, 4종을 조합을 달리하여 처리하였을 때 뚜렷한 협력작용 증가효과를 가져오지 못했다. 이상의 결과에서 다른 협력제들은 곤충의 방어기작을 알라타체를 포함하여 비선택적으로 저해하는 것으로 생각되었으나 RO20-9747은 7-ethoxy precocene II가 알라타체로 이행하는데 까지 곤충의 방어적 산화기작인 monooxygenase 활성을 선택적으로 저해함으로써 7-ethoxy precocene II가 알라타체로 이행량이 증가하여 활성증가를 가져온 것으로 생각되었다. The second instar milkweed bugs, Oncopeltus fasciatus were exposed to the residue of 6-methoxy-7-ethoxy-2, 2- dimethylchromene(or 7-ethoxy precocene 11, 7-EP-11) with one, two, or four different kinds of synergist(s) deposited as a residue on 9 cm diameter petri dishes. The anti juvenile hormone analog, 7-EP-I1 yielded a 50% effectiveness concentration at 1.18 wg/cmZ. The concentration of 7-EP-I1 and R020-9747 combined gave 0.084 ~*g/cm', as much 14 times more potent than with 7-EP-I1 alone. Most of the other synergists, including geraniol, 4-chalcone oxide, isosafrole, and piperonyl butoxide however, showed comparatively low level synergism with 1.0-4.3 times, depending upon the combinations of the synergist(s). The obtained results were considered that R020-9747, an analog of benzopyran with similar structure to that of 7-EP-I1 inhibited selectively the insect monooxygenase oxidative defense mechanism, in the body except for corpora allata because general antioxidants of piperonyl butoxide, isosafrole, and 4-phenyl chalcone oxide showed relatively mild synergism.
몇 가지 살충제의 썩덩나무노린재와 뚱보기생파리에 대한 독성
정부근,임유진,이흥수,박정규,Chung, Bu-Keun,Lim, Eugene,Lee, Heung-Su,Park, Chung Gyoo 한국응용곤충학회 2014 한국응용곤충학회지 Vol.53 No.4
몇 가지 살충제에 대한 독성을 썩덩나무노린재를 대상으로 단감원에서 잔효독성으로 검증하였고 끈끈이 트랩에 유인된 뚱보기생파리 성충을 대상으로 직접분무처리 방식으로 검증하였다. 썩덩나무노린재는 흑색유아등으로 채집하였고 뚱보기생파리 성충은 갈색날개노린재 집합페로몬인 methyl-(E,E,Z)-2,4,6-decatrienoate을 사용해서 끈끈이트랩으로 포획하였다. 비펜트린수화제, 뷰프로페진 디노테퓨란수화제, 클로티아니딘액상수화제, 디노테퓨란수화제, 티아메톡삼입상수화제 등 5종의 살충제 중에서 비펜트린수화제가 72시간 케이지 내 잔효독성 검정결과 썩덩나무노린재에 대해 93.1%의 사충율을 나타내었고 여타 살충제들은 다양한 사충율을 보였다. 뚱보기생파리에 대한 사충율은 55.3~74.3%이었다. Toxicity of several insecticides was tested against the brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), and adult Gymnosoma rotundatum (Diptera: Tachinidae) in a sweet persimmon orchard. H. halys was captured using black light traps and G. rotundatum adults were collected using sticky traps lured with an aggregation pheromone, methyl-(E,E,Z)-2,4,6-decatrienoate of Plautia stali. Five insecticides, namely, bifenthrin 2 WP, buprofenzine+dinotefuran (20+15) WP, clothianidin 8 SC, dinotefuran 10 WP, and thiamethoxam 10 WP, were moderately toxic to G. rotundatum, resulting in 55.3% to 74.3% mortality. Bifenthrin 2 WP caused 93.1% mortality at 72 h within cages as a residual toxicity, and others caused varied mortalities.
정부근,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1983 中央醫大誌 Vol.8 No.1
The effect of acid-base on the electrical and mechanical properties of myocardial cells were investigated in the isolated right ventricular papillary muscle preparations of rabbits by measuring simultaneously isometric contraction and the action potential by glass microelectrode transducer in acidic or alkali conditions respectively. Results were as follow : 1. The acid condition decreased the isometric contraction and at the sametime increased the time to repolarize 90% of its action potential amplitude.(Repol 90%) from 234±9.1 msec of the control to 244±6.8msec(P<0.01). 2. The alkali condition, on the other hand, increased the isometric contraction and lengthened Repol 90% from 256±49.3 msec of the control to 286±75.2msec(P<0.1) but Repol 95% lengthened more prominently from 281±66.8msec of the control to 329±101.5(P<0.05). The above result suggests that acid and alkali decreased and increased slow inward current respectively to reduce and augment the isometric contraction. In acidic condition, the reduction in delayed outward current might also play a role in augmented action potential duration.
오이에서 살충제 계열내 교호처리에 의한 온실가루이 방제 체계
정부근,손경애 한국응용곤충학회 2001 한국응용곤충학회지 Vol.40 No.4
원예작물 중 온실가루이의 발생이 가장 심한 오이를 대상작물로 선정하여 온실가루이에 대한 기존의 살충제를 이용한 화학적 방제체계를 수립하기 위하여, 중후기에 방제효과와 잔효독성, 엽중 농약 잔류량 조사, 방제횟수 절감, 농약 저항성 발달 및 오이의 수량 등 온실가루이 방제체계 확립에 필요한 기초적인 사항을 조사하였다. 조사결과 온실가루이에 대하여 가장 효과적인 살충제는 furathiocarb였고 특히 이 약제는 carbofuran으로 더욱 강력한 활성화 과정을 거치면서 온실가루이의 저항성 발달이 비교적 더디게 나타났고 따라서 방제효과가 길고 결과적으로 방제횟수도 줄이는데 기여하는 것으로 판명되었다. 이와는 달리 기존의 다른 약제들에 대해서는 합성피레스로이드계의 약제, deltamethrin, etopenprox, zetacypermethrin 등에 대해서는 저항성 발달이 빨리 나타났고, 일부 유기인계 약제, methidathion, phenthoate, profenofos 등의 경우에는 살포 후 분해가 빨리되어 약효의 상실이 빨라짐으로 인해서 결과적으로 살포회수를 늘여야하는 것으로 나타났다. 이들 기존 살충제들에 대한 저항성 온실가루이의 발생으로 방제가 곤란한 경우에 acetamiprid가 대체 약제로서 유망하였다. In order to establish a whitefly control system using conventional groups of insecticide(carbamate, organophosphorus and pyrethroid insecticides), three alternative application methods were designed on the medium growth stage of cucumber. To discriminate the effectiveness of these sequences observed were the residual activity of insecticides, frequency of insecticide applications, residue of insecticides in cucumber leaves, development of insecticide resistance in whitefly, and yield of fruits. Spraying furathiocarb, a carbamate insecticide, was very effective in reducing the frequency of application for the control of white flies. The effectiveness of furathiocarb was enhanced by the potentiation process to carbofuran, the long residual activity, and the lower development rate of insecticide resistance. Methion, an organophosphorus insecticide, did not show resistance development after successive use but resulted in short residual activity. However, other organophosphates, profenofos and phenthoate, lost their activity by the resistance development. Decreasing activity was common to pyrethroids, deltamethrin and zetacypermethrin due to resistance. From these results it could be drawn a conclusion that furathiocarb, a carbamate insecticide, was the most desirable among conventional insecticide groups for the management of greenhouse whitefly population on the cucumber. To prevent an outbreak of the insect pest by various cause, it was recommended to choose acetamiprid, a nicotinoid, which showed very good control efficacy to the resistance insects to conventional insecticides.
Sampling of Bemisia tabaci adults using a pre-programmed autonomous pest control robot
정부근,XIA CHUNLEI,송유한,이장명,Yan Li,김흥수,전태수 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4
An autonomous robot (Cabbot)was built to control insect pests in a plastic greenhouse and utilized to sample adultwhiteflies (Bemisia tabaci) on paprika plants (Capsicum annum var. angulosum). To accomplish this, a sampling deviceconsisting of an air compressor for pest agitation, a sticky trap (100mm×150mm) and an image processingsystem for pest identification were installed on the Cabbot. The sampling precision of the Cabbot (D= 0.16)washigher than that of the sticky trap (D= 0.19) when sampling of adultwhite flies was conducted in caged pots. TheCabbot could also collect a substantial number of individuals within a short duration (3 minutes). The collection efficiency(i.e., percent of samples collected at least n (1 in this case) individuals to the total sample number) of insectsby the Cabbot was markedly high at low population size, showing approximately 30% when the populationsize was ≤16 individuals per plant. The sampling precision and collection efficiency suggested that the Cabbot iseffective in local, short-term sampling and could be used for early warning of pest occurrences.
붉은테두리진딧물(Rhopalosipnum rufiabdominalis (Sasaki))에 의한 매실나무 신초 피해 및 방제적기
정부근,Chung, Bu-Keun 한국응용곤충학회 2010 한국응용곤충학회지 Vol.49 No.3
매실나무에 발생하는 붉은테두리진딧물의 경제적 방제법을 확립하기 위하여 잎 피해양상을 경남 하동에서 조사하였고, 경남농업기술원내 포장에서 방제적기를 구명하기 위하여 acetamiprid WP를 2007년 4월 3일부터 시작하여 5월 8일까지 매주 살포하였다. 붉은테두리진딧물의 매실나무 잎 피해는 5월 상순부터 발생하기 시작하여 5월 중순에 가장 많았고, 그 이후 점차 낮아졌으나 10월 중순까지 지속되었다. 또한 붉은테두리진딧물의 방제적기는 2007년의 경우 4월 17일로 잎은 여전히 연약하고 3-5장의 잎이 전개되고 있는 상태였다. Occurrences of Rhopalosipnum rufiabdominalis damage in the leaves of Japanese apricots were observed in Hadong, Gyeongnam Province, a major producing area. R. rufiabdominalis occurred from early May to mid October, with the peak occurrence of damaged leaves in mid May. To find proper timing of chemical control against R. rufiabdominalis, acetamiprid WP was sprayed weekly from 3 April, before budbreak, to 8 May in 2007. The best time for control R. rufiabdominalis was on 17 April 2007, at which the leaves were still tender and developing up to 3 to 5 leaves.