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        大七氣湯이 抗癌, 免疫調節作用 및 apoptosis에 미치는 影響

        하지용,정병억,이선구,유병길 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.1

        Daechilgitang, extracted from Sparganium stoloniferum Buch. Ham, Curcuma zedoaria rosc, Aurantii immatri pericarpium, Fraxini cortex, Platycodon grandiflorum (jacg.) DC., Agaslache rugosa(Fisch.et Mey.) kuntze, Trigonella foenum-graecum L., Cyperus rotundus L., Cinnamomum cassia BI, Glycyrrhiza uralensis Fisch., is known to antitumor agent. This study was performed to investigate antitumor, immune response, and apoptotic effects by Daechilgitang in the cancer lines and Balb/c mice. Experimental studies were progressed through the anticancer activities such as, life extention, cell cytotoxicity, natural killer cell activity, productivity of interleukin-2, apoptotic effects. The results were summarized as follows: 1.Median survival time in Daechilgitang-treated group was prolonged to 16%, as compared with control group. 2.On the MTT assay, half-maximal inhibitory concentration(IC50) of Daechilgitang on cell lines, were 532.3㎎/㎖ in sarcoma-180 cell, and 2.8㎎/㎖ in HT-29 cell. 3.Natural killer cell activity in Daechilgitang-treated group was significantly increased in case of 100:1 effect cell/target cell ratio. 4.Production of interleukin-2 in Daechilgitang-treated group was significantly increased. 5.On the studies of Daechilgitang-induced apoptosis, a DNA fragmentation patterns and electron microscopic appearance were evident, however, activity of MAP kinase was not affected. Key word : Daechilgitang, IC50, anti-tumor, immunomodulatory, interleukin-2, natural killer cell activity, apoptosis, DNA fragmentation, MAP kinase activity

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