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전수환,김지훈,최홍열,강승훈,남형진,김지연,김동일 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.5
Various polymers were used as transfection factors for small interfering RNA (siRNA) to effectively suppress human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) gene in transgenic rice cells. Five kinds of polymers (PEI, PVA, PVP, and 8 and 20 kDa PEGs) were applied for delivery of siRNA with lipofectamine used as a control. In the cytotoxicity test, all polymers except 8 kDa PEG showed nontoxicity in relation to cell viability. For transfection efficiency, polyplexes composed of siRNA and PEG (20 kDa) did not significantly reduce production of intracellular hCTLA4Ig. On the other hand, siRNA + PEI polyplexes showed the most effective suppression efficiency with regards to production of intracellular hCTLA4Ig among all other polyplexes (PVA, PVP, and PEG (8 kDa)). Effects of molecular weight ratios of siRNA:PEI were investigated to obtain optimal transfection efficiency and avoid excessive damage to cells. PEI-based polyplexes with a 1:10 ratio of siRNA:PEI reduced production of intracellular hCTLA4Ig up to 70.6% without alteration of cell viability. These results demonstrate that PEI-based polyplexes are easy to prepare, inexpensive, non-toxic, and effective to deliver siRNA to transgenic plant cell cultures.
전수환,안성아 한국예술종합학교 무용원 2023 무용과 이론 Vol.7 No.-
This study introduces a real case of analyzing the taste of paid audiences at a domestic complex performance hall. For analysis, a survey was conducted on the audience of a large complex performance hall that performs various genres. First, the audience segmentation analysis was conducted by dividing the sample of respondents into three groups(heavy users, medium users, and light users) according to the amount of viewing. Light users were characterized by viewing popular performing arts. Heavy and medium users showed omnivore characteristics that cross pure and popular art. Second, as a result of market segmentation according to taste based on the multi-genre viewing behavior of audiences(heavy users and medium users), it was confirmed that there were “pure art audience group,” “public performance audience group,” and “classical music audience group.” “public performance audience group,” and “classical music audience group.” There were various types according to the audience's taste, and genre changes were occurring in different ways depending on the taste, focusing on classical music, pure performing arts, and popular performing arts. In the future, it is expected that this study can be used to establish appropriate marketing strategies by segmentation analysis based on actual performance audience data.