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전소영,김현태,권세윤,김정식,김범수,유은상,권태균 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.4
Upper urinary tract-derived urine stem cells (USCs) are considered a valuable mesenchymal stem cell source for autologous cell therapy. However, the reported culture condition for USCs is not appropriate for large-quantity production, because cells can show limited replicativity, senescence, and undesirable differentiation during cultivation. These drawbacks led us to reconstitute a culture condition that mimics the natural stem cell niche. We selected extracellular matrix protein and oxygen tension to optimize the ex vivo expansion of USCs, and compared cell adhesion, proliferation, gene expression, chromosomal stability, differentiation capacity, immunity and safety. Culture on collagen type I (ColI) supported highly enhanced USC proliferation and retention of stem cell properties. In the oxygen tension analysis (with ColI), 5% O2 hypoxia showed a higher cell proliferation rate, a greater proportion of cells in the S phase of the cell cycle, and normal stem cell properties compared to those observed in cells cultured under 20% O2 normoxia. The established reconstituted condition (ColI/hypoxia, USCsrecon) was compared to the control condition. The expanded USCsrecon showed highly increased cell proliferation and colony forming ability, maintained transcription factors, chromosomal stability, and multi-lineage differentiation capacity (neuron, osteoblast, and adipocyte) compared to the control. In addition, USCsrecon retained their immune-privileged potential and non-tumorigenicity with in vivo testing at week 8. Therefore, reconstituted condition allows for expanded uUSC cell preparations that are safe and useful for application in stem cell therapy.
전소영,송후캉,황연숙 한국실내디자인학회 2022 한국실내디자인학회논문집 Vol.31 No.5
The flagship store is positioned as an effective public relations tool that provides a unique brand experience rather than simply selling products. In today’s standardized commercial environment, the flagship store reflects various lifestyles of customers and provides a space to satisfy their consumption needs. The purpose of this study is to present data on how to link regional expression characteristics to the current flagship store. As a research method, theoretical contents on the spatial characteristics and regional expression characteristics of the flagship store were considered, and a field survey was conducted on eight flagship stores in Seoul. The results of the study are as follows. First, although flagship stores are an open space that generally receives abundant light, the natural environmental factors were not well introduced. In order to apply more natural elements, a terrace or rooftop could be improved by, and a garden or walking path would provide a new natural environment in the region. Second, even when the harmony with the surrounding area of the store is high, it can be difficult to identify the facade. Therefore, by using exterior color, formative elements, or typography, designs can be improved to increase attention and arouse the curiosity of passers-by. Third, the cultural space that can promote the community of consumers in the district is insufficient. To this end, an open or variable space should be included to use the space more efficiently and to perform various cultural roles.
비대칭 6상 영구자석 동기 전동기의 스위치 개방에 따른 특성 분석 및 고장허용운전
전소영,황선환,박종원 한국산업융합학회 2022 한국산업융합학회 논문집 Vol.25 No.6
This paper proposes a method related to fault tolerance operation and characteristic analysis of asymmetric 6-phase permanent magnet synchronous motor. In general, motor drive systems with multi-phase structures can be continuously operated despite a reduction of power and speed by using a phase changeover or control techniques according to the failures. As a result, it is widely used in industrial fields such as aviation and defense, which require high efficiency and high reliability. In this paper, the second order ripple of the electrical fundamental freuqnecy occurs in the dq-axis currents of the synchronous coordinate system through mathematical analysis according to the switch open of the dual 3-phase inverter. Therefore, the fault tolerant operation method is presented by applying the fault detection method with a constant cycle for continuous operations. The effectiveness of the proposed fault tolerance operation method is verified through the several experiments.
Analysis of Stability of Human Urine Derived Stem Cells during Serial Subcultures
전소영,박강백,권태균,최석환 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.4
This study was conducted to assess stabilities of the human urine derived stem cells (USCs) during serial subcultures to demonstrate clinical feasibility for future stem cell therapy. For this work, we compared to cell morphology, proliferation rate, immunophenotype, multilineage differentiation potentials and immunomodulatory effect of USCs. USCs maintained cell morphology, cell proliferation ability through serial culture (till passage 7). In immunophenotypical analysis, USCs revealed positive for embryonic/mesenchymal stem cell marker (stage specific embryonic antigen, SSEA4) and mesenchymal stem cell markers (CD44, CD73, CD90 and CD105), while negative for hematopoietic stem cell markers (CD34, CD45 and C-kit) and immunogenic marker (HLA-DR). The USC had multi-differentiation potencies into adipocytes and osteocytes, and these abilities maintained into passage 7. The immunomodulatory effect of USCs with phytohemagglutinin stimulated peripheral blood mononuclear cell proliferation was analyzed with two systems, co-culture or separated culture system. The peripheral blood mononuclear cells’ proliferation was effectively inhibited on both systems, and the value was more effective with co-culture system than separate one. The present study suggests that sequential sub-passages till passage 7 could maintain stem cell characteristics, multipotent properties, and immune suppressive effect of USCs which should be considered in their therapeutic application in regenerative medicine.