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기내배양한 은행 유식물에서의 Ginkgolide의 생산
전미희(Mee Hee Jeon),성상현(Sang Hyun Sung),전순화(Soon Hwa Jeon),허훈(Hoon Huh),김영중(Young Choong Kim) 한국생약학회 1994 생약학회지 Vol.24 No.4
A platelet activating factor(PAF) antagonist ginkgolides produced from Ginkgo biloba are well known for their potential usage in septic shock and other PAF related diseases. Even though they are extracted from the leaves and on occasion the root bark, the exact biosynthetic site and pathway have not proved yet. In order to locate the enzymes involved and elucidate the biosynthetic site of the compounds, embryo-derived aseptic intact plantlet and plantlet without root have been cultured on 0.3% active carbon-containing solid Murashige and Skoog`s medium. The leaves from the six-week-old normal plantlet contained similar amount of ginkgolide B to that of outdoor plant leaves, while the plantlets without root had less than 30% of the ginkgolide B compared to the in vitro intact plantlets. The results suggest that the ginkgolides may be synthesized in the root and transported to the aerial part.
인삼 Saponin이 혈장 Lipoproteins 및 Lecithin Dispersion과 Dextran Sulfate의 상호작용에 미치는 영향
김영중(Young Choong Kim),전미희(Mee Hee Jeon) 한국생약학회 1984 생약학회지 Vol.15 No.4
The effect of saponin fractions of Panax ginseng root on the interactions of human plasma lipoproteins and lecithin dispersions with dextran sulfate were studied in order to examine the effect of Panax ginseng on the lipid accumulation in the aorta. The total saponin fraction and protopanaxadiol glycosides of Panax ginseng root seemed to slightly enhance the interaction of low density lipoproteins with dextran sulfate in the absence of divalent metal ions. Protopanaxatriol glycosides remarkably inhibited the interaction of low density lipoproteins with dextran sulfate. However, all of these three saponin fractions of Panax ginseng root showed the tendency of inhibition to the interaction of high density lipoproteins with dextran sulfate in the presence of divalent metal ions by the order of protopanxatriol glycosides, protopanaxadiol glycosides and total saponin. Three saponin fractions of Panax ginseng exerted almost same tendency to the interaction of lecithin dispersions with dextran sulfate in the presence of divalent metal ions as the interaction of low density lipoproteins with dextran sulfate absence of divalent metal ions.
현호색의 Acetylcholinesterase 활성 저해 성분 및 그 작용기전
황세영,장영표,변순정,전미희,김영중,Hwang, Se-Young,Chang, Young-Pyo,Byun, Soon-Jung,Jeon, Mee-Hee,Kim, Young-Choong 한국생약학회 1996 생약학회지 Vol.27 No.2
In the course of searching for acetylcholinesterase inhibitors from crude drugs, it was found that total MeOH extract of Corydalis Tuber showed significant inhibitory effect on acetylcholinesterase. To isolate acetylcholinesterase inhibitors from Corydalis Tuber, total MeOH extract of the the crude drug was subjected to activity guided fractionation. The MeOH extract was suspended in water and fractionated with methylene chloride and subjected to acid-base fractionation. Silica gel column chromatography of the basic fraction which showed significant inhibitory effect on acetylcholinesterase was carried out and 5 subfractions (1-5) were obtained. From subtraction 4, compound I was isolated. The structure of isolated compound I was identified by spectroscopic parameters of $^1H-NMR$, $^{13}C-NMR$, EI-MS and FAB-MS. The compound I was identified as berberine. It was found from the Lineweaver-Burk plot that berberine was a reversible and specific inhibitor of acetylcholinesterase having 90% inhibitory effect at the concentration of $2.5{\mu}M$.
글루타메이트에 의한 신경독성에 미치는 징코라이드의 영향
김소라(So Ra Kim),전미희(Mee Hee Jeon),김영중(Young Choong Kim) 대한약학회 1996 약학회지 Vol.40 No.6
The neurotoxicity induced by L-glutamate in primary cultures of rat cortical cells could be attenuated by diterpene constituents of Ginkgo biloba leaves, ginkgolides A, B and C. At the concentration of 100nM, ginkgolides up-regulated the activity of glutathione reductase in primary cultures of rat cortical cells exposed to 100mcM glutamate. Furthermore, ginkgolides increased the content of reduced glutathione in glutamate-treated cortical cells. However, ginkgolides showed little effect in reducing superoxide dismutase activity. Ginkgolides did, however, markedly block the production of malondialdehyde, a byproduct of lipid peroxidation in glutamate-treated rat cortical cells.