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Oxytetracycline과 neomycin 복합제의 약욕에 따른 양식 넙치(Paralichthys olivaceus)의 근육내 잔류량 변화
김승민,전려진,정준범,Kim, Seung Min,Jun, Lyu Jin,Jeong, Joon Bum 한국어병학회 2015 한국어병학회지 Vol.28 No.1
본 연구에서는 넙치 (평균체장 및 무게: $20{\pm}2.0cm$, $70{\pm}5.0g$)에 옥시테트라사이클린(oxytetracycline, OTC)-네오마이신(neomycin, N) 복합제를 25-10 ppm 및 50-20 ppm으로 용해하여 2일 동안, 3시간씩 약욕을 실시하였고, 약물투여 중지 후 1일, 5일, 14일, 40일째에 시험군별로 각각 5마리씩 넙치의 근육을 채취하여 항생제 잔류농도를 조사하였다. OTC와 N 표준용액을 농도별로 희석하여 표준곡선을 작성한 결과 각각 $R^2=0.9999$, $R^2=0.9952$로 양호한 직선성을 나타내었고, OTC와 N 회수율은 각각 90~93%, 88~95%가 관찰되었다. 잔류농도에 대한 측정결과, OTC와 N 복합제를 25-10 ppm 투여한 시험군에서 1일째 OTC는 $0.97{\pm}0.084{\mu}g/ml$이 검출되었고, N은 $0.118{\pm}0.079{\mu}g/ml$이 검출되었으나 5일째 이후부터 검출이 되지 않았다. OTC와 N 복합제를 50-20 ppm 투여한 시험군에서 1일째 OTC는 $1.324{\pm}0.062{\mu}g/ml$이 검출되었고 N은 $0.788{\pm}0.05{\mu}g/ml$이 검출되었으나 5일째 이후부터 검출이 되지 않았다. In the present study, we performed a dipping of olive flounder (average length and weight: $20{\pm}2.0cm$, $70{\pm}5.0g$) for a period of three hours a day, over two days, in a melted complex of oxytetracycline (OTC) and neomycin (N), by dissolving 25-10 ppm or 50-20 ppm in water. Subsequently, the remaining antibiotic density in muscle tissue collected from olive flounder was investigated, 1, 5, 14 and 40 days after discontinuation of the medication. 5 fish were used from each group. The standard graph drawn from the results of diluting two standard solutions of OTC and N based on various density levels, showed a relatively straight line with an $R^2$ of 0.9999 and 0.9952, respectively. The recovery rate of OTC was shown to be 90-93% and N, 88-95%. Upon measurement of the remaining antibiotic density in the test group that had been exposed to 25-10 ppm of the complex of OTC and N, $0.97{\pm}0.084{\mu}g/ml$ of OTC and $0.118{\pm}0.079{\mu}g/ml$ N were detected on 1 day of the test. No antibiotic density was detected after day 5 of the test. Regarding the test group that were exposed to 50-20 ppm of the complex of OTC and N, $1.324{\pm}0.062{\mu}g/ml$ of OTC and $0.788{\pm}0.05{\mu}g/ml$ N were detected on day 1 of the test, and no antibiotic density was detected after day 5 of the test.
어류 병원성 세균의 MIC 결정을 위한 MIC Panel의 최적화 배양 조건 확립
김예지,전려진,강미래,이다원,우수지,김명석,정준범 한국수산과학회 2020 한국수산과학회지 Vol.53 No.3
No established method can be used to select effective antibiotics in antibiotic susceptibility tests for fish bacterialpathogens quickly and accurately. Here, we established the optimal conditions for determining the minimal inhibitoryconcentration (MIC) of major fish bacterial pathogens (Streptococcus spp., Edwardsiella tarda, Vibrio spp., Aeromonasspp., and Pseudomonas spp.) using the KRAQ1 and CAMPY2 panels. The MIC panel used 18 antibiotics of twotypes and we conducted experiments to establish the optimal culture medium and temperature for each species. Theoptimal conditions for incubating Streptococcus spp. were in cation-adjusted Mueller-Hinton broth with TES buffer(CAMHBT) at 28°C, using 5% lysed horse blood (LHB) as recommended by the Clinical Laboratory StandardsInstitute. For Vibrio spp., the optimal culture conditions were 28°C in CAMHBT supplemented with 1% NaCl. Theoptimal conditions for culturing E. tarda, Aeromonas spp., and Pseudomonas spp. were in CAMHBT at 28°C
제주지역 양식 넙치(Paralichthys olivaceus)를 대상으로 한 스쿠티카증(Scuticociliatosis) 모니터링
고예진,전려진,김예지,이영준,한지은,오영은,이응준,정준범 한국어병학회 2023 한국어병학회지 Vol.36 No.1
Scuticociliatosis can occur from juveniles to adult olive flounder (Paralichthys olivaceus) and the infection usually causes mass mortality. There is no clear way to treat it once the parasite has invaded the fish's internal organs. Therefore, periodic monitoring of scuticociliatosis for surveillance and prompt response is a crucial part of reducing damage to olive flounder farms. In this study, we conducted scuticociliatosis monitoring of olive flounder farms in jeju island to investigate the detection rate of the causative agents (Miamiensis avidus and Uronema marinum), and determine the phylogenetic classification of the detected pathogens. Disease monitoring was conducted on a total of 3,282 olive flounder and the detection rate was highest in August (15.83%) during the investigation period. The sequences of all 4 strains obtained from olive flounder farms in 4 different regions, were 100% homologous with SSU rRNA gene of M. avidus deposited in GenBank.
양식 넙치에서 분리한 어병세균의 lincomycin에 대한 내성 유전자의 분포
김예지,전려진,이영준,고예진,한소리,김성현,정준범,Kim, Ye Ji,Jun, Lyu Jin,Lee, Young Juhn,Ko, Ye Jin,Han, So Ri,Kim, Sung Hyun,Jeong, Joon Bum 한국어병학회 2022 한국어병학회지 Vol.35 No.1
Lincomycin as one of the lincosamides antibiotics have been mainly used in human and livestock fields, but have not been used in aquaculture. In this study, the distribution of minimum inhibitory concentration (MIC) values against lincomycin and the detection of the macrolide-lincosamide-streptogramin (MLS) resistance gene were confirmed in bacterial pathogens isolated from cultured olive flounder (Paralichthys olivaceus). Of the 107 strains isolated from Jeju, 36 strains of Gram-positive bacteria and 71 strains of Gram-negative bacteria were identified. Most of Streptococcus spp. was found to have a MIC value of less than or equal to 0.5 ㎍/mL, and Edwardsiella piscicida was found to have a MIC value higher than 1,024 ㎍/mL. V. harveyi and V. alginolyticus mostly showed MIC values of 256 ㎍/mL, but V. scophthalmi displayed values of 8~64 ㎍/mL. In the detection of MLS resistance gene, erm(B) was detected in 9 strains of Streptococcus spp., and erm(A) was confirmed in one strain.