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강낭콩과 대두 잎에서 세포간액 키틴분해효소의 분포와 유도
박노동(Ro Dong Park),조유영(Yu Young Cho),전덕영(Deok Young Jhon) 한국응용생명화학회 1994 Applied Biological Chemistry (Appl Biol Chem) Vol.37 No.4
Using the enzyme activity staining, we studied the induction and distribution of chitinase isozymes, pathogenesis-related proteins, in intercellular fluids of bean and soybean leaves under stress conditions. The chitinase in intercellular fluids was barely detected in healthy plant leaves. By treatment of ethylene, pathogen (Fusarium oxysporum), or wounding, only 34 kD intercellular endochitinase was induced in bean leaves, while 30 kD and 36 kD intercellular endochitinases were induced in soybean leaves.
강낭콩 잎에서 스트레스에 따른 키틴분해효소의 특이적 유도
박노동,이춘명,전덕영 ( Ro Dong Park,Choon Myong Lee,Deok Young Jhon ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.2
We studied the induction of chitinase (EC 3.2.1.14), a pathogenesis-related protein, in bean plants under stress conditons. The chitinase was barely detected in healthy plant leaves but was significantly induced by ethylene, pathogen Fusarium oxysporum, or wounding, which was tested by enzyme activity assay, western blotting, and activity staining. Six isozymes of bean chitinase were separated in 12.5% SDS-PAGE. They were differentially induced when bean plants were treated with ethylene, F. oxysporum, or wounding.