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콩과 식물에서 분리정제한 렉틴의 생물물리 화학적 연구 ( E - PHA 렉틴 )
전경희,채영흠,서영아,정시련 ( Kyung Hee Jeune - Chung,Young Heum Chae,Young Ah Suh,See Ryun Chung ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.1
Salt fractionated extracts of Phaseolus vulgaris was purified successively through ion exchange chromatography on DEAE Sephadex A-50, hydroxyapatite column and EL-PHA was obtained. EL-PHA was treated with 4M urea just prior to the application into CM-Sephadex C-50 column. This column was eluted with 10 mM (pH 5.7), 20 mM (pH 6.6) and 30mM (pH 8.0) phosphate buffer, each containing 4M urea. In order to identify isolation of E-and L-PHA, erythroagglutination test with human blood A group and lymphoagglutinating test with mouse spleen lymphocytes were measured on each fraction and found three active fractions, L-PHA, having lymphoagglutinating activity only, EI-PHA, having high erythroagglutinating activity only and EII-PHA, having erythroagglutinating activity along with weak lymphoagglutinating activity. In order to characterize biophysicochemical properties of the EI-and EII-PHA, polyacrylamide disc gel electrophoresis, SDS gel electrophoresis, sugar inhibition test, carbohydrate test were performed. In polyacrylamide disc gel electrophoresis, EL-PHA was observed as three bands, EI-and EII-PHA were observed as clear single band with a very faint minor band. Molecular weight of EI-and EII-PHA were estimated at about 130,000 daltons and 125, 000 daltons, respectively by PAGE. The results of SDS-PAGE shown that EI-PHA consists of four homogenous subunits having molecular weight of 34, 000 per unit and EII-PHA consists of two different subunits with 41,000 arid 30,000 daltons. Erythroagglutination by EI-and EII-PHA was inhibited with 0.3% D-mannose and N-acetyl-D-galactosamine, but not with N-galactose, L-fucose, N-acetylneuraminic acid and N-acetyl-D-glucosamine. Sugar components of EI-and EII-PHA were recognized by anthrone test, but no spot was observed in paper chromatography. For immunochemical studies, antisera to EI-PHA were prepared by immunizing rabbit and EI-PHA formed precipitin bands against various antisera in Ouchterlony double immunodiffusion.
전경희,박채수,박원학,최수정,소명숙,정시련 영남대학교 약품개발연구소 1997 영남대학교 약품개발연구소 연구업적집 Vol.7 No.-
A new lectin was partially purified from starfish. Asterina pectinifera by means of physiological saline extraction, salt fractionation, ion exchange chromatography and hydroxyapatite chromatography, and it was named APL. The biochemical properties of the APL were characterized. In addition, its effects on lymphocyte mitogenicity and cancer cell agglutinability were tested. The APL agglutinated nonspecifically human erythrocytes and rabbit blood cells, Agglutinability was decreased to 30% of control activity below pH 5 and above pH 9 and was relatively unstable at increasing temperatures above 60℃. The activity was reduced by addition of two kinds of metal ions. Ba^(2+), Mn^(2+) and chelating agent. EDTA. APL was proved to be glycoproteins containing 9% sugars. For carbohydrate specificity, it was found that the activity of APL was inhibited by D(+)-glucosamine. D(+)-galactosamine, stachyose, N-acetyl-galactosamine and methyl-??-D-galactopyranoside among 35 sugars tested. In amino acid composition, the contents of acidic amino acids such as aspartic acid and glutamic acid were relatively high. This result suggest that the isoelectric point would be in a lower range. APL was found that in promotes the division of human lymphocytes. APL was proved to be a potent agglutinin for cancer cells such as HeLa, L929 and L1210 cells. Significant changes on the HeLa cell surfaces affected by APL were observed under the electron microscope.
해양동물 렉틴 ( 제 11 보 ) : 조각매물고둥 렉틴의 림프구 분열효과 및 면역화학적 특성
전경희,서영아,소명숙,정시련 ( Kyung Hee Jeune,Young Ah Suh,Myung Suk So,See Ryun Chung ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.3
Developing new substances for immunosuppressor or immunomodulator from natural products is extremely important in the present biomedicine. In this paper, we focused our efforts on the mitogenicity and immunochemical properties of the NI lectins (NIA, NIB, NIC), obtained from Neptunea intersculpta. Immunochemical techniques were employed to elucidate the structural and/or functional similarities between the NI lectins. According to these results, NIB and NIC were almost identical and the NIC proved to be homogeneous on immunoelectrophoresis as well as crossed immunoelectrophoresis. But these lectins are turned out as different molecules through the study of carbohydrate specificity. Only NIC lectin was a potent mitogenic toward murine splenic lymphocytes at a concentration ca. 6.4 ㎍/㎖.
창의융합인재 선발을 위한 인성교육 및 인성평가 요소 분석
전경희,김은하 한국융합학회 2017 한국융합학회논문지 Vol.8 No.2
본 연구는 창의융합인재 선발을 위한 인성교육 및 인성평가의 요소를 탐색하기 위해 예비대학생을 대상으 로 한 설문조사를 실시하였다. 연구 결과, 초중등 교육과정에서 타인배려, 대인관계능력, 정직성 관련 인성교육 프로 그램이 가장 높은 빈도로 운영되고 있는 것으로 조사되었다. 또한 인성교육 프로그램 효과 및 중요도에 대해 대인관 계능력, 타인배려, 성실성 순으로 프로그램 효과가 높으며 중요하게 인식하고 있는 것으로 나타났다. 성별에 따른 인식의 차이를 비교한 결과, 대학생을 대상으로 하는 인성교육의 필요성과 대입 전형에서 인성이 평가요소로 반영 될 필요성에 대한 인식에서는 성별에 따른 차이가 유의하게 나타났다. 이러한 연구 결과를 토대로 중·고등학교의 인성 교육과정과 대학 입학전형 제도 개선을 위한 정책 제언을 제시하였다. This study aimed to examine character evaluation criteria and components for selecting creative convergent talents in college admissions. In this study, pre-college students’ perceptions were surveyed on various issues related to character education and evaluation implemented in K-12 school system. It was found that the character education programs intended to enhance students’ care for others, interpersonal skills, and honesty were most actively operated within the secondary educational systems. The results of the study also indicated that the programs for fostering interpersonal skills, care for others, and sincerity were perceived as the most effective programs. Based on the results of the study, we discussed ways to improve the quality of character education programs operated in school curriculum and to improve the credibility of character evaluation in the college admission process.