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닭 전염성 기관지염에 관한 연구: II. 간접 enzyme-linked immunosorbent assay(ELISA)에 의한 항체가 측정
장정호,김선중,Chang, Chong-ho,Kim, Sun-joong 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.4
Critical parameters affecting sensitivity and specificity of an enzyme-linked immunosorbent assay(ELISA) for detection of antibodies to avian infections bronchitis virus(IBV) were standardized. By adopting the optimized conditions an equation calculating ELISA antibody titers from the observations at single serum dilution was formulated. The purified antigen of IBV Mass-41 strain was dispensed into polystyrene microplate wells at a concentration of 300ng per well($100{\mu}l$) and the plates were coated by completey drying at $37^{\circ}C$. Diluted chicken serum and horseradish peroxidase conjugated goat anti-chicken IgG were added in order in $100{\mu}l$ volumes per well and allowed to react for 30 minutes each at room temperature. Just before use and after each reaction the plates were washed three times with distilled water. Finally o-phenylenediamine solution was added as an enzyme substrate. After incubation for another 15 minutes at room temperature absorbances were read at 492nm. Hyperimmune serum against Mass-41 strain was used as internal reference positive(IRP) serum. After repeated titration of IRP and negative serum, a constant titer of IRP was determined. Serum titrations were carried out for various sample sera together with IRP and negative sera and the observed titers of sample sera were corrected by reflecting the ratio between observed and constant titers of IRP serum. These corrected titers of the sample sera were plotted against sample/positive(S/P) OD ratios. All the OD's measured in the serum titrations were also corrected by substracting negative serum OD. The following equation was formulated from the above data; $Log_{10}$ ELISA titer=$5.568({\log}_{10}S/P)+4.161$ Thus it was possible to calculate ELISA titer by measuring absorbance at 1/400 single serum dilution. Titer measured by cross ELISA tests employing Mass-41 strain and three local IBV isolates were similar. These results suggest that the ELISA tests standardized in this study can be used for evaluating not only vaccinal immunity but also for infection status against fields IBV's.
장정호,장병탁 한국뇌학회 2003 한국뇌학회지 Vol.3 No.1
문서 집합 내의 개념 또는 의미 관계의 자동 분석은 보다 효율적인 정보 획득과 단어수준 이상의 개념 수준에서의 문서 비교를 가능하게 한다. 이를 위해 은닉변수모델을 이용하여 문서 집합으로부터 단어들 간의 의미 관계를 자동적으로 추출하고 이를 통해 문서 간 유사도 측정을 효과적으로 하기 위한 방안을 제시한다. 은닉변수모델로는 다중요인모델의 학습이 용이한 헬름홀츠 머신을 활용하며 이의 학습 결과에 기반하여, 문서 간 비교를 위한 커널(semantic kernel)을 구축한다. 4개의 표준 문서 집합에 대한 검색 실험에서 제안된 기법을 적용함으로써 기본 VSM(Vector Space Model)에 비해 20% 이상의 평균 정확도 향상을 이룰 수 있었다. The automatic analysis of concepts or semantic relations in document sets enables the comparison of documents in the concept level other than the simple word level. In this research, we present a method based on latent variable models to automatically extract the semantic relationships between various words and to estimate the similarity among documents effectively. We use Helmlioltz machines, a latent variable model that makes ease of the learning of multiple cause models for the automatic text analysis, and construct semantic kernels for the estimation of document similarity. In the retrieval experiment for four standard document sets, our methods shows a significantly improved performance, over 20% compared with the basic VSM, in term of average accuracy.