Evans 증후군 1례

장성익,이의재,황성수,이경일,이원익,이준성 최신의학사 1990 最新醫學 Vol.33 No.1

人類 染色體 硏究

張性翼 啓明大學校 醫科大學 1982 계명의대학술지 Vol.1 No.1

Interleukin-2 Receptor의 활용 using LAK cells

장성익 啓明大學校 醫科大學 1989 계명의대학술지 Vol.8 No.1

휴지기(G_0 phase)에 있던 T임파구는 항원이나 분열촉진제 등에 의하여 세포환(cell cycle)내로 들어가서 TFR(transferrine receptor)의 도움을 받아 DNA를 합성하고 세포분열을 한다. 이때 항원이나 분열촉진제(PHA등)등에 의해 자극을 받으면 interleukin-2가 분비되는데 이 물질은 호르몬과 비슷한 성장 인자이다. 이때 IL-2에 대한 특수 수용체인 interleukin-2 receptor가 발현한다. IL-2는 항암 면역요법의 본체로 최근에 매우 각광을 받고 있는 물질이다.대체로 IL-2는 T임파구를 증식시키는 작용외에 B임파구의 증식, LAK세포 및 NK세포의 유도 뿐만 아니라 어떤 조양에서는 면역을 억제 시키기도 하고 현재는 암유전자(특히 c-myc)와 관계가 있는 것으로 알려지고 있다.

암의 세포유전학

장성익 啓明大學校 醫科大學 1991 계명의대학술지 Vol.10 No.1

Now, cancer is a disease of gene which can be classified to somatic cell genetic diseases. Cancer might be resulted from a relative genetic insufficiency to tolerate carcinogen exposure in major oncoderm. The balance between exogenous stimulus(carcinogen exposure) and constitutional responce(capacity to resist or repair mutational damage) is altered in favor of the DNA- damaging tendency, and cancer develops. It has been found that the cancer-relevant genes were located on the specific regions of chromosomes. Specific chromosome abnormalities were discovered in leukemia and malignant lymphoma. However, most of solid tumours were not still investigated in specific karyotypes for the specific cancers. Oncogenes are corelated to sites of translocations on the chromosome and antioncogenes are corelated to locus of deletions on the chromosome in cancer cells. Aneuploidy is a phenomenon of cancer progressing which is revealed on the secondary change of mitosis. Recently multistep oncogenesis theory which is concerned with oncogenes, antioncogenes and genomes of the chromosomes has been attractive point of view in explanstion of cancer developing. To understand this hypothesis investigators should approach to cytogenetics. Cancer cytogenetics, even still obscure, may be applied to clinical diagnosis or prediction of prognosis from the patient. I believe that analysis of karyotypes can predict the cancer susceptability from normal state of individuals in the future.

Significance of Cancer Cytogenetics

장성익 啓明大學校 醫科大學 1989 계명의대학술지 Vol.8 No.2

Sister Chromatid Exchange(SCE)의 意義

張性翼,朱剛 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.1

사람의 임파구를 배양하여 BudR 10㎍/㎖와 Hoechst 33258 50㎍/㎖로 처리하여 Giemsa 염색한 결과 전염색체에서 SCE가 나타났다. Sister chromatid exchange (SCE) events which previously have been detected by autoradiography can now be detected by BudR with Hoechst 33258 technique. SCE has been proved to the highly sensitive index of the interaction of carginogens with chromosomes, and abnormalities related to SCE formation occurred in a number of hereditary diseases known or suspected to involve a defect in DNA repair. Blood leukocytes were cultured according to conventional methods. BudR (10㎍/㎖) was added to culture medium 3 hours before colchicine treatment. The lymphocytes incorporated with BudR were stained in Hoechst 33258 and followed Giemsa stain. The result obtained is that SCE seems to be found out on all the chromosomal numbers including sex chromosome.

^3H―thymidine Autoradiography에 의한 숫병아리 胃粘膜上皮細胞의 Cell Cycle에 關하여

張性翼,朱剛 慶北大學校 醫科大學 1972 慶北醫大誌 Vol.13 No.2

In recent years, it has been believed that the DNA synthetic time (S-phase) is between 7 and 9 hours, however, the reports on this subject were vastly variable and also variable on G_2-phase (postsynthetic time) We presumed that S-phase would not be constant but S+G_2 phase would be constant. And we estimated the percent of labeled mitosis with one day postnatal chick stomach, and calculated as followings: 1. The S-phase was 4.5 hours, G_2-phase was 8.8 hours. 2. Minimum S-phase was 3 hours. 3. The total generation time was 19.5 hours and similar with the other reports, and the second curve was irregular, so it was computed by means of a formula using the labeling index and S-phase. It could be concluded that there were some relations between shortened S-phase and prolonged G_2-phase, and S+G_2phase were constant, however, it is needed more consideration and further study.

암관련 유전자

장성익 계명대학교 의과대학 1991 계명의대학술지 Vol.10 No.2

Cancer may be a disease of genes, arising from genetic damage of diverse sorts-recessive and dominant mutations, large rearrangement of DNA and gene translocation on chromosomes, all leading to distorsions of either the expression or biochemical function of genes. The search for these genetic damage in neoplastic cells now is the most important in cancer research. The oncogenes, appear to act dominantly, may directly induce neoplastic growth in experimental animals or malignant transformation in appropriately selected cultured cells. The antioncogenes are recessive at the level of individual cells operating through gene inactivation in tumorigenesis. The carcinogenic process have multistep involving in oncogene and antioncogene at least in the solid cancers. Cancer genes may have roles in regulating differentiation or in the maintenance of fully differentiated cells in normal cells. It is needed now to understand the roles of cancer genes in embryogenesis. Because some genes between relevant to teratology and relevent to oncology have quite genes to clinical tools for early diagnosis, prognosis and therapy.

實驗時間의 差異에 따른 Cell Cycle의 變動에 對하여

長性翼,朱剛,李壽廣,尹健鎬 慶北大學校 醫科大學 1974 慶北醫大誌 Vol.15 No.2

저자는 ^3H-thymidine에 의한 cell cycle 연구에 있어서 circadian rhythm의 영향을 관찰코져 生後 第1 日 숫병아리 肝細胞를 대상으로 다음과 같은 성적을 얻었다. 1. 오전 6시에 주사한 실험군에서 세대시간은 110.6시간이었으며, DNA合成期는 7.3시간이고 G_2기는 3.3 시간, 분열기는 0.1시간이었고 G_1기는 99.9시간 이었다. 2. 오후 3시에 주사한 실험군에서 세대시간은 83.0시간이었으며 DNA合成期는 7.0시간이고 G_2기는 2.3시 간, 분열기는 0.5시간이었고 G_1기는 73.2시간이었다. 3. 오전 6시에 희생한 실험군에서 세대시간은 239.0시간이었고 S期는 10.5시간, G_2期는 2.5시간, G_1期 는 225.0시간이었고 M기는 1.0시간 이었다. 4. 오후 3시에 희생한 실험군에서 세대시간은 530.0시간이었고 S期는 10.6시간, G_2期는 2.8시간, G_1期 는 516.0시간이었고 M기는 1.2시간 이었다. 5. 各 실험군의 표지지수는 오전주사군이 6.6%, 오후주사군이 8.4시간, 오전희생군이 4.4%, 오후희생군 이 2.0%로서 有意한 차이를 보였다. 6. 세대시간은 희생군이 주사군에서보다 훨씬 연장되어 circadian rhythm의 영향을 인정할 수 있었다. It is very improtant to set the adquate experimental time in the measurement of cell cycle. According to our laboratory data of experimental time there are many variations in the results of data and these variations seem to have relation with circadian rhythm. The animals were divided into four experimental groups: A M injection, P M injection, A M sacrificed, and P M sacrificed groups. ^3H-thymidine was introduced into one day postnatal male chicks of Shaver species and autoradiography was carried out on the liver cell. The results were summurized 1. The cell cycles of live cells in four experimental groups revealed distinct circadian rhythmicity. 2. The durations of T_G of A M and P M injection groups, and sacrifice groups were 110.6, 83.0, 239.0 and 530.0 hours respectively. 3. The S phase was about 7 hours in injection groups and about 10 hours in sacrifice groups. 4. The G2 phases were 2-3 hours in all the experimental groups. 5. The durations of G_1 phase of A M and P M injection and sacrifice groups were 225.0 and 516.0 hours respectively. 6. The labelling indices of A M and P M injection and sacrifice groups were 6.6, 8.4, 4.4, and 2.0% respectively. 7. Circadian variation was most distinct in G_1 phase and S phase showed remarkable circadian variation.

비 인두암에서 EB-virus의 검출 및 비 인두암과 EB-virus로 전형시킨 B 림프구의 세포유전학적 비교

장성익,김홍태,이인환,최인장,송달원 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.1