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포도상구균 장내 C 형 변이독소 (SEC mutant)의 면역원성에 대한 연구
장병선,주이석,문진산,서근석,양수진,김소현,박용호,Chang, Byoung-sun,Joo, Yi-seok,Moon, Jin-san,Seo, Keun-seok,Yang, Soo-jin,Kim, So-hyun,Park, Yong-ho 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.2
Mastitis is one of the most significant cause of economic loss to the dairy industry. Especially, Staphylococcus aureus is a major contagious mastitis-causing pathogen in dairy cattle. Because of its high transmission rate and resistance to antibiotic therapy, staphylococcal mastitis presents a constant threat to the dairy industry. Staphylococcal enterotoxin C(SEC) produced by S aureus has been known as one of superantigens which are able to stimulate a large proportion of T lymphocytes independently of their antigenic specificity. In this experiment, we have conducted preliminary studies with mice and lactating cows to evaluate the immunogenicity and safety of the experimental vaccine consists of SEC mutant antigen on controlling the bovine mastitis associated with S aureus infections. The average value of somatic cell counts in quarter milk, isolation rate of S aureus were consistently decreased in SEC-SER vaccinated groups, whereas antibody titers were highly increased in SEC-SER vaccinated groups. Peripheral blood were also collected from the lactating cows to determine the proportion of leukocyte subpopulation associated with humoral immunity(HI) and cell mediated immunity(CMI). Proportion of leukocyte subpopulation expressing $BoCD2^+$(total T lymphocyte), $BoCD4^+$(T helper cell), $BoCD8^+$(T cytotoxic/suppressor cell) and NonT/NonB lymphocyte which are involved in CMI in SEC-SER vaccinated groups were decreased for the initial stage after first vaccination and then increased from ten weeks after first vaccination maintaining elevated level till 14 weeks after vaccination. In contrast, proportion of monocyte, MHC class II and B lymphocyte which are associated with the production of primary immune response in SEC-SER vaccinated groups were increased for the initial period and then decreased from ten weeks after first vaccination. We present evidence that vaccination of SEC-SER mutant antigen in lactating cows induced a significant proliferation of bovine T lymphocytes. These results suggest that SEC-SER mutant antigen used in this experiment might be one of potential immunogen in developing innovative vaccine against bovine IMI associated with S aureus. Additional challenge trials should be carried out to evaluate substantial protection against S aureus under the commercial farm conditions.
장병선 대한설비공학회 2003 설비저널 Vol.32 No.9
지하철 승강장내에서 승객의 안전확보, 쾌적한 역사환경조성, 에너지절약 등이 요구되는 시설을 소개하고자 한다.
유전자재조합 pST 투여가 돼지의 성장생산성 및 육질에 미치는 영향
정정수,장병선,권관,최명자,최정은,제훈성,김남중 한국축산학회 1997 한국축산학회지 Vol.39 No.5
The current study was undertaken to evaluate the effects of a locally-produced recombinant porcine somatotropin (pST) on growth perfermance and pork quality of finishing pigs. A total of 32 barrows weighing average 61kg were employed for the 6-week growth trial : 8 barrows were allocated into the control group (CONT): 8 barrows into the slow release-low dose pST group (SR-L); 8 barrows into the solve release - high dose pST group (SR-H); 8 barrows into the daily pST group(DAILY). CONT pigs received no pST. SR-L and SR-H pigs were treated with pST twice during 6 week trial(Each dose contained the pST equavalent to 50 ㎍ and 100 ㎍ per day, respectively). DAILY pigs received 100 ㎍ pST/㎏ B W daily. All pigs were fed a commercial feed containing 0.87% lysine ad lib. There was no significant difference in average daily gain among treatments. Feed/gain was reduced by 20% in DAILY (3.09 vs 3.85; p$lt;0.05) and 11% in SR-H (3.43 vs 3.84; p$lt;0.05) compared to the CONT. Backfat of the DAILY group was 20% thinner than that of the CONT and loin eye area was wider by 17% (p$lt;0.05) in DAILY group, compared to the CONT. The extent of the improvement in growth perfermance of SR groups was less (p$lt;0.05) than that of DAILY group. There were no significant differences in the color, juiciness, flavor and texture of pork among treatments, with the tenderness of DAILY group being less desirable compared to that of CONT group. Moisture content of DAILY group was higher (p$lt;0.05) than that of CONT group, while lipid content was reduced in DAILY group. SR group showed the middle values between CONT and DAILY group in most criteria on growth performance and pork quality investigated. These results indicated that the recombinant pST produced by LG Chem., Ltd., Korea stimulated growth performance in growing pigs and did not show any major negative effects on pork quility, suggesting that slow release pST could be used as a growth stimulant for growing pigs.
Peroxidase와 Lipoxygenase에 대한 농약의 저해작용
황인영,장병선,박관화,노정구,Hwang, In-Young,Chang, Byeong-Seon,Park, Kwan-Hwa,Roh, Jung-Koo 한국환경농학회 1984 한국환경농학회지 Vol.3 No.2
화학물질(化學物質)에 의(依)한 서양고추냉이와 사과의 peroxidase 및 정제된 대두(大豆) lipoxygenase 역가저해(力價沮害)를 조사(調査)하였다. 서양고추냉이와 사과의 peroxidase 역가(力價)에 저해효과(沮害效果)를 주는 농약중(農藥中) maneb가 zineb의 효과(效果)보다 컸으며 10,20,30ppm의 처리농도(處理濃度)에서 maneb는 12.5,23.0,34.5%, zineb는 9.5, 17.5,27.5%의 역가저해(力價沮害)를 보였다. Maneb와 zineb의 혼합체(混合體)인 mancozeb 를 500 ppm의 농도(濃度)로 사과나무에 철포(撤布)했을때 사과의 peroxidase 역가변화(力價變化)는 무처리구(無處理區)와 별 차이(差異)가 없었다. 대두(大豆)의 lipoxygenase 역가(力價)에 미치는 영향(影響)은 zineb의 경우 가장 저해도(沮害度)가 컸으며 phosphamidon과 carbofuran 이 또한 저해효과(沮害效果)가 있었다. 그러나 maneb는 저해효과(沮害效果)가 없었다. 10, 20, 30 ppm의 처리농도하(處理濃度下)에서의 lipoxygenase 역가저해도(力價沮害度)는 zineb가 14.0, 27.0, 40.0%, carbofuran이 6.5, 12.5, 20.0% 그리고 phosphamidon이 4.5, 9.0, 13.0%였다. 또한 이들 세농약(農藥)은 $100{\sim}200\;ppm$에서 100% lipoxygenase 역가저해(力價沮害)를 보였다. The effect on the activities of peroxidase and lipoxygenase by chemicals was determined. Peroxidase activities of horseradish and apple were inhibited strongly by maneb and comparativelyby mancozeb, zineb. The inhibitory rate of enzyme activity was ranged in $12.5{\sim}34.5%$ by maneb, $11.0{\sim}30.5%$ by mancozeb, and $9.7{\sim}27.5%$ by zineb in the level of $10{\sim}30\;ppm$ at the final concentration. No signification was in the peroxidase activity of apple during ripening between control and plot, treated with 500 ppm mancozeb. On the activity of purified soybean lipoygenase, zineb inhibited it more strongly than carbofuran or phosphamidon, but maneb did not have inhibitory effect on that. The inhibition ranges of $14.0{\sim}40.0%$ by zineb, $6.5{\sim}20.0%$ by carbofuran and $4.5{\sim}13.0%$ by phosphamidon were shown in the final pesticide concentration of $10{\sim}30$ ppm. But in $100{\sim}200$ ppm, lipoxygenase activity was almost inhibited by carbofuran and phosphamidon.