척수타박 손상 후 BDNF 단백질 발현과 기능적 회복에 미치는 트레드밀 운동의 효과

장문녀(Moon-Nyeo Jang),오명진(Myung-Jin Oh),정일규(Il-Gyu Jeong) 한국생활환경학회 2009 한국생활환경학회지 Vol.16 No.2

Physical activity and exercise can improve sensorimotor recovery after central nerve injury. Among neurotrophic factors, brain-derived neurotrophic factor (BDNF) is known to modulate several neuronal functions including cell survival. Yet, a correlation between the induction of these proteins in non-neuronal cells and functional recovery is largely unknown. Seven-week-old male Sprague-Dawley rats were divided into three groups to know the effects of treadmill exercise on BDNF expression and functional recovery after spinal cord contusion in the rats; normal(intact)group(N=15), SCI(sedentary)group(N=15), and SCI+TMTgroup(N=15). Training group(TMT) is exercised on a treadmill maintained by a 30-min run at 0° inclination five times for a week during seven weeks one week after SCI. Here, we report that the low-intensity treadmill training (TMT) in rats upregulates BDNF protein levels in nonneuronal cells in the injured spinal cord and further facilitates axonal regrowth. Western blot analyses showed that levels of BDNF were increased in injury site 8 weeks after spinal cord contusion, and further upregulated by TMT. Enhancement of these protein in each region after TMT in contused rats was associated with proliferation of astrocytes by 8 weeks post injury. The extent of axonal sprouting in the injury site regions was higher in TMT group than sedentary group. Gridwalk tests were used to evaluate behavioral improvement after spinal cord contusion. All behavioral scales were improved in TMT group compared with sedentary group. Thus, the present data provide a new evidence that increased BDNF activity in proliferating astrocytes after spinal cord injury may play an important role in TMT-mediated enhancement of functional recovery in the injured central nerve.

뇌 타박 손상 후 해마에서 신경세포 생성 및 신경 가소성 조절 단백질 발현에 미치는 운동 기간에 따른 효과

장문녀 ( Moon Nyeo Jang ),백일영 ( Il Young Paik ),윤진환 ( Jin Hwan Yoon ),서태범 ( Tae Beom Seo ) 한국스포츠정책과학원(구 한국스포츠개발원) 2011 체육과학연구 Vol.22 No.3

이 연구의 목적은 외상성 뇌손상 후 해마에서 신경세포 생성과 신경 가소성에 관여하는 단백질들에 대한 운동 기간에 따른 효과를 규명하는 것이다. 이 연구에서는 7주령 Sprague-Dawley 계열의 수컷흰쥐(n=70)를 사용하여, 무선배치 방식으로 통제군(n=10), 외상성 뇌손상 대조군(7, 14 그리고 28일, n=10) 그리고 외상성 뇌손상 후 운동군(7, 14 그리고 28일, n=10)으로 분류하였다. 외상성 뇌손상은 electromagnetic controlled inpact 장비에 의해 유도되었다. 트레드밀 운동은 7, 14 그리고 28일동안 일주일에 5일, 하루에 30분씩 8m/min의 속도로 수행하였다. 외상성 뇌손상 후 손상 범위를 비교분석 확인하기 위해 H&E 염색법을 수행하였고, 모든 그룹에서 통계적인 유의성은 나타나지 않았다. western blot을 수행하기 위해 모든 그룹에 해마를 적출하였다. 외상성 뇌손상 후 해마에서 BDNF, synapsin I 그리고 p-CREB의 발현은 정상군에 비해 감소하였지만, 손상 후 7, 14 그리고 28일 동안의 규칙적인 트레드밀 운동은 BDNF, syanpsin I 그리고 p-CREB의 발현을 통계적으로 유의하게 증가시켰다. 또한 운동에 의한 synapsin I과 p-CREB의 증가 패턴은 BDNF의 변화와 비슷한 경향을 보였다. 이러한 결과들을 종합해 볼때, 트레드밀 운동은 외상성 뇌손상 후 해마의 신경세포 생성 및 신경 가소성을 증가시키는 중요한 매개체임을 알 수 있다. This study aims to investigate effects of time window for exercise on neurogenesis- and synaptic plasticity-regulated proteins in the hippocampus after traumatic brain injury (TBI). Male SD rats, aged 7 weeks, were randomly assigned to the following three groups: Sham-operation group (n=10), TBI group (7, 14 and 28 days post injury, n=10) and TBI and exercise group (7, 14 and 28 days post injury, n=10). TBI was induced by a electromagnetic controlled impact. Treadmill exercise was conducted with duration and frequency of 30 minutes and 5 times per week, respectively, for 7, 14 and 28 days. H&E staining was used for comparing injury area in all TBI groups and there was not different significantly. Hippocampus in all groups was prepared and used for western blot. In our results, although TBI resulted in decrease of BDNF, synapsin I and p- CREB induction levels in hippocampus of the brain, exercise for 7, 14 and 28 days after TBI significantly enhanced expression levels of these proteins. Increase of synapsin I and p-CREB showed trendency similar to exercise-regulated BDNF. Based on the results, it can be suggested that treadmill exercise provide a beneficial effect on neurogenesis and synaptic plasticity in hippocampus of rat with TBI.

척수타박손상 후 트레드밀운동이 근 위축지연과 기능적 회복에 미치는 효과

오명진(Myung-Jin Oh),장문녀(Moon-Nyeo Jang),서태범(Tae-beom Seo),김종오(Jong-Oh Kim),변재종(Jae-Jong Byun),윤진환(Jin-Hwan Yoon),정일규(Il-Gyu Jeong) 한국생명과학회 2009 생명과학회지 Vol.19 No.11

본 연구는 흰쥐의 흉추 9번을 타박 손상시키고 1주간의 휴식 후 스트레스를 유발하지 않는 저강도 트레드밀운동을 7주동안 수행하여, 가자미근 위축지연 변화를 western blotting과 H&E 염색 그리고 면역형광염색법으로 관찰하였으며, BBB-검사를 이용하여 기능적 회복을 관찰하였다. 형태학적 변화의 결과 척수손상 후 트레드밀 운동에 의해 하지부위 가자미 근과 근섬유의 횡단면 크기가 증가하였으며, 근섬유 세포에서 IGF-Ⅰ단백질 발현이 증가하였다. 또한 생화학적인 결과 척수손상 후 트레드밀 운동에 의해 하지부위 골격근 IGF-Ⅰ단백질 발현이 증가하였으며, 행동학적 검사를 통해 트레드밀 운동그룹이 비 운동그룹보다 기능적 회복이 개선된 것을 확인하였다. 이러한 결과는 근 비대에 직접적으로 관여하는 IGF-Ⅰ단백질 발현량이 운동에 의해 증가하여 가자미근크기 증가와 같은 근 위축 지연을 확인하였으며, 이러한 변화를 통해 기능적 회복에 긍정적인 결과를 보여주었다. 따라서 운동은 척수손상 환자에게 효과적인 기능재활 치료방법이 될 수 있으며, 장애를 최소화하여 자발적인 일상생활을 도모할 수 있을 것이라 생각된다. Physical activity and exercise can promote sensorimotor recovery from central nerve injury. It has been suggested that the functional recovery promoted by exercise training after spinal cord injury might be associated with insulin-like growth factor-Ⅰ in the inflicted muscle. To investigate morphological and biochemical change of the soleus muscle after spinal cord injury, all tissues were used for H&E, immunofluorescence staining and Western blot. Also, BBB-test was used to evaluate behavioral improvement after spinal cord contusion. Thirty male Sprague-Dawley rats (230±10 g; 7week in age) were assigned equally to three different groups; Normal (n=10), SCI (n=10), SCI+TMT (n=10). Every rat in SCI and SCI+TMT groups underwent laminectomy at T9 level and then contusion on the exposed spinal cord site in anesthetized condition. After one week-recovery from contusion, every rat in the SCI+TMT group exercised on a motorized treadmill for 30min/d, 5d/wk for 7wks. TMT followed by injury increased IGF-Ⅰ induction levels in the soleus muscle and inhibited muscle atrophy. Behavioral scales for 4 and 8 weeks after spinal cord injury were improved in the SCI+TMT group compared to the SCI group. These results suggest that treadmill exercise after spinal cord injury might promote functional recovery along with muscle regrowth through the up-regulation of IGF-1 in muscle tissue.

직장건강증진프로그램이 근로자들의 건강 체력 및 혈청지질에 미치는 영향

박수양(Soo Yang Park),채정룡(Jeong Ryong Chae),장문녀(Moon Nyeo Jang) 한국사회체육학회 2008 한국사회체육학회지 Vol.0 No.34

The purpose of this study was to exam the effect of shift-workers` Workplace health promotion for health strength and blood, and to verify need of Workplace health promotion. The program was being tested on 39 male workers without physical defect for 12 weeks. They were divided into 3 groups, Group Exercise(GE), Personal Exercise(PE) who used gym of outside company, Control group(CG). Statistics system was practiced of two way repeated measured ANOVA by repeated measurement and a significant level was set up as .05. As a result of this research, BMI, VO2 max, Sitting Trunk Flextion of GE were improved significantly (P<.05), Sit up and Back muscular strength of GE and PE were improved significantly (P<.05). Also in lipid profiles, TC, HPL, LDL, TG of GE were improved significantly(P<.05). That is, Workplace health promotion for shift-workers could improve health strength and lipid profiles of workers.

상대적 수영운동 강도가 흰쥐 갈색지방조직의 UCP-1과 UCP-3 mRNA 발현, 혈중 인슐린 및 혈당에 미치는 효과

윤진환(Jin-Hwan Yoon),오명진(Myung-Jin Oh),서태범(Tae-beom Seo),김종오(Jong-Oh Kim),장문녀(Moon-Nyeo Jang),박성태(Seong-Tae Park),김영표(Young-Pyo Kim),유재현(Jae-Hyun Yoo) 한국생명과학회 2009 생명과학회지 Vol.19 No.2

본 연구에서는 F344계 흰쥐를 대상으로 8주간 저강도 운동군과 고강도 운동군으로 나누어 수영운동을 실시하여, 갈색지방조직 내 UCP-1과 UCP-3mRNA 발현을 관찰하고 혈당 및 인슐린 수준이 어떠한 변화를 나타내는지 알아보았다. 그 결과 저강도 수영을 실시한 그룹이 대조군과 고강도 운동그룹보다 갈색지방조직 내 UCP-1과 UCP-3 mRNA 발현이 증가되는 것을 관찰하였으며, 고강도 수영군에서 대조군 보다 인슐린 수준이 낮게 나타났으나 혈당에서는 유의한차가 나타나지 않았다. 하지만 저강도 수영군에서 대조군보다 혈당 및 인슐린 수준이 유의하게 감소하는 것을 관찰하였다. 이러한 결과는 저강도 수영운동이 갈색지방조직 내 UCP-1과 UCP-3mRNA 발현을 증가시키고, 당대사를 활성화하여 인슐린민감도를 개선시킬 수 있음을 보여주는 결과이다. The purpose of this study was to investigate the UCP-1, UCP-3 mRNA expression in brown adipose tissue with glycometabolism according to intensity and duration of swimming in rat. F344 rat were randomly divided into three groups (n=10 in each group): control (CON), low-intensity swimming (LIS) groups, and high-intensity swimming (HIS) groups. Animals in the LIS group were forced to swim in swimming pool for 30min once a day for 8 consecutive weeks with a light intensity. In the HIS group, the rats repeated fifteen 20-s swimming bouts with a weight equivalent to 10% of body weight for 8weeks, respectively. The present result demonstrated that in LIS group, serum insulin and glucose levels significantly decreased in LIS group compared to CON. Brown adipose tissue UCP-1 and UCP-3mRNA expression was significantly increase in LIS group compared to CON and HIS groups. From those results, it can be suggested that low-intensity swimming may improve glycometablism control by up-regulating UCP-1 and UCP-3mRNA expression.