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인천광역시에서 유통되는 계란의 플루오로퀴놀론계 항균물질 잔류 실태 조사
박은정 ( Eun Jeong Park ),임지흔 ( Ji Huen Lim ),이성모 ( Sung Mo Lee ),임정수 ( Jeong Soo Im ),오대규 ( Dae Kyu Oh ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.3
This study was carried out to investigate the residue level of fluoroquinolones in hen`s general eggs and specific eggs by microbiological assay method and high performance liquid chromatography (HPLC) method. HPLC separation was carried out by reversed phase chromatography on a Symmetry C18 (250 × 4.6 ㎜, 5 ㎛ particle size) with a phase composed of distilled water (containing 0.4% triethylamine and phosphoric acid) : Methanol (780 : 220, v/v), pumped isocratically at a flow rate of 1.0 ㎖/min. A fluorescence detector was utilized with an excitation wavelength of 278 ㎚ and an emission wavelength of 456 ㎚. The calibration curves were linear (γ2≥0.999) over a concentration range of 0.025~0.4 ㎍/㎖. Average recoveries of the five fluoroquinolones in whole eggs at fortified levels of 0.05~0.2 ㎍/g were ranged mean 78.1~91.7% and low coefficient of variation was less than 10% for all analysed samples. The limits of detection and limits of quantification for whole eggs were 1.2~6.0 ng/g and 2.3~9.1 ng/g, respectively. Only one hen`s general eggfrom chicken farm in Incheon was detected with the residual fluoroquinolones (Microbiological assay method ; 1 of 47 general eggs) ; the range of residual concentration enrofloxacin was 0.12 ppm. Those in food stores were detected with the residual fluoroquinolones (Microbiological assay method ; 4 of 88 general eggs) ; the ranges of residual concentration enrofloxacin were 0.15~2.2 ppm, ciprofloxacin 0.01~0.06 ppm, and hen`s specific eggs (40) in food stores were not detected. For the microbiological assay method of fluoroquinolones in hen`s eggs, as the results of comparative analysis, the disc diffusion method with E coli may be a little highly detected for the residual fluoroquinolones.
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