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Gene expression profile analysis in cultured human neuronal cells after static magnetic stimulation
임우석,이순태,김승찬 한국바이오칩학회 2012 BioChip Journal Vol.6 No.3
Although the magnetic force has been used in various human environments and medicines, their influence on the nervous system has not been fully elucidated. In this study, we investigated mRNA expressions profiles of neuronal cells after the application of static magnetic fields. Two perpetual magnets were applied to the cultured SH-SY5Y human neuronal cell, and the gene expression profiles were evaluated by using human mRNA microarray targeting 30968 genes. Results showed that the expressions of 827-known genes were altered in response to the magnetic force. Among them, 112 genes showed significant changes(>2-fold changes); 44 genes were up- regulated and 68 genes were down-regulated. Among the up-regulated genes, we further confirmed the increased expressions of synapsin III and chloride channel-2 by using RT-PCR and immunocytochemistry. These re-sults suggest that static magnetic fields influence neuronal-and biological related gene expression profiles in human neuronal cells.
Sun Ginseng Protects Endothelial Progenitor Cells From Senescence Associated Apoptosis
임우석,정진영,반재준,임지연,이순태,주건,김만호 고려인삼학회 2012 Journal of Ginseng Research Vol.36 No.1
Endothelial progenitor cells (EPC) are a population of cells that circulate in the blood stream. They play a role in angiogenesis and, therefore, can be prognostic markers of vascular repair. Ginsenoside Rg3 prevents endothelial cell apoptosis through the inhibition of the mitochondrial caspase pathway. It also affects estrogen activity, which reduces EPC senescence. Sun ginseng (SG),which is heat-processed ginseng, has a high content of ginsenosides. The purpose of this study was to investigate the protective effects of SG on senescence-associated apoptosis in EPCs. In order to isolate EPCs, mononuclear cells of human blood buffy coats were cultured and characterized by their uptake of acetylated low-density lipoprotein (acLDL) and their binding of Ulex europaeus agglutinin I (ulex-lectin). Flow cytometry with annexin-V staining was performed in order to assess early and late apoptosis. Senescence was determined by β-galactosidase (β-gal) staining. Staining with 4′-6-Diamidino-2-phenylindole verifi ed that most adherent cells (93±2.7%) were acLDL-positive and ulex-lectin-positive. The percentage of β-gal-positive EPCs was decreased from 93.8±2.0% to 62.5±3.6% by SG treatment. A fl uorescence-activated cell sorter (FACS) analysis showed that 4.9% of EPCs were late apoptotic in controls. Sun ginseng decreased the apoptotic cell population by 39% in the late stage of apoptosis from control baseline levels. In conclusion, these results show antisenescent and antiapoptotic effects of SG in human-derived EPCs, indicating that SG can enhance EPC-mediated repair mechanisms. Endothelial progenitor cells (EPC) are a population of cells that circulate in the blood stream. They play a role in angiogenesis and, therefore, can be prognostic markers of vascular repair. Ginsenoside Rg3 prevents endothelial cell apoptosis through the inhibition of the mitochondrial caspase pathway. It also affects estrogen activity, which reduces EPC senescence. Sun ginseng (SG),which is heat-processed ginseng, has a high content of ginsenosides. The purpose of this study was to investigate the protective effects of SG on senescence-associated apoptosis in EPCs. In order to isolate EPCs, mononuclear cells of human blood buffy coats were cultured and characterized by their uptake of acetylated low-density lipoprotein (acLDL) and their binding of Ulex europaeus agglutinin I (ulex-lectin). Flow cytometry with annexin-V staining was performed in order to assess early and late apoptosis. Senescence was determined by β-galactosidase (β-gal) staining. Staining with 4′-6-Diamidino-2-phenylindole verifi ed that most adherent cells (93±2.7%) were acLDL-positive and ulex-lectin-positive. The percentage of β-gal-positive EPCs was decreased from 93.8±2.0% to 62.5±3.6% by SG treatment. A fl uorescence-activated cell sorter (FACS) analysis showed that 4.9% of EPCs were late apoptotic in controls. Sun ginseng decreased the apoptotic cell population by 39% in the late stage of apoptosis from control baseline levels. In conclusion, these results show antisenescent and antiapoptotic effects of SG in human-derived EPCs, indicating that SG can enhance EPC-mediated repair mechanisms.
A Study of the Housing Needs of the aged and Elderly Residential Facilities in an Urban Area
임우석 서울대학교행정대학원 2007 The Korean Journal of Policy Studies Vol.21 No.2
The purpose of this study is to find out the demand for residence ofcitizens on the paid elderly residential facilities that has been heightened for itsuse in recent days with the focus on the paid nursing home facilities and the paidelderly treatment facilities. The subject for the survey was 200 adults of 35 yearsor older who reside in Seoul, and for the final analysis, a total of 169 copies ofvalid samples was used. The following is the major result of the study. For thereason of post-retirement entry into the nursing home facilities, the mostresponses came from the fact that they could live with the similar age people asneighborhood or friend. Appropriate residential area would be preferred for 7-12pyeong (1 pyeong=3.3058m2) for single use, and 12-15 pyeong for couples, andfor the appropriate security deposit and monthly living expense at the time ofentering into the facilities, the highest response was for 50 million won and500,000 won for the paid nursing home facilities, and 10-20 million won and600,000 won for the paid elderly treatment facilities. The most important criteriafor using the facilities was in the sequence of entry costs, facilities and others,and the consideration on the facility operator would be in the sequence of facili-ty operation capability and social reliability. And, for the annexation (coestab-lishment) of paid nursing home facilities and elderly treatment facilities, theopinion as desirable was shown high (73.9%).
Applications of CRISPR/Cas9 for Gene Editing in Hereditary Movement Disorders
임우석,문장섭,김만호 대한파킨슨병및이상운동질환학회 2016 Journal Of Movement Disorders Vol.9 No.3
Gene therapy is a potential therapeutic strategy for treating hereditary movement disorders, including hereditary ataxia, dystonia, Huntington’s disease, and Parkinson’s disease. Genome editing is a type of genetic engineering in which DNA is inserted, deleted or replaced in the genome using modified nucleases. Recently, clustered regularly interspaced short palindromic repeat/CRISPR associated protein 9 (CRISPR/Cas9) has been used as an essential tool in biotechnology. Cas9 is an RNA-guided DNA endonuclease enzyme that was originally associated with the adaptive immune system of Streptococcus pyogenes and is now being utilized as a genome editing tool to induce double strand breaks in DNA. CRISPR/Cas9 has advantages in terms of clinical applicability over other genome editing technologies such as zinc-finger nucleases and transcription activator-like effector nucleases because of easy in vivo delivery. Here, we review and discuss the applicability of CRISPR/Cas9 to preclinical studies or gene therapy in hereditary movement disorders