단양쑥부쟁이에 함유된 Caffeoylquinic Acid의 HPLC 분석과Peroxynitrite 소거효과

임상철,박희준 한국자원식물학회 2016 한국자원식물학회지 Vol.29 No.1

Five caffeoylquinic acids of Aster altaicus var. uchiyamae Kitamura (Compositae) leaves were identified using standard compounds by HPLC and determined as follows: 3,4-di-O-caffeoylquinic acid (4.92 ± 0.06 ㎎/g dried weight), 3,5-di-O-caffeoylquinic acid (3.95 ± 0.13 ㎎/g), 4,5-di-O-caffeoylquinic acid (1.39 ± 0.10 ㎎/g), 5-O-caffeoylquinic acid (chlorogenic acid, 8.05 ± 0.21 ㎎/g), 3-O-caffeoylquinic acid (4.97 ± 0.18 ㎎/g). The total content of five caffeoylquinic acids were calculated as 26.73 ± 0.26 ㎎/g dried weight while the percentage of the five compounds in the MeOH extract was calculated as 25.22 ± 0.25%. The IC50 value of the MeOH extract scavenging peroxynitrite (ONOO - ) was shown as 5.16 ± 0.15 ㎍/㎖. Key words - Aster altaicus, Compositae, Caffeoylquinic acid, Peroxynitrite, HPLC 적 요단양쑥부쟁이의 caffeoylquinic acid 성분을 HPLC로 분석하였다. 그 결과 3,4-DQ, 3,5-DQ, 4,5-DQ, 5-CQ, 3-CQ 5종 화합물이 검출되었다. 이 식물의 caffeoylquinic acid 총 함량은 26.73 ± 0.26 ㎎/g으로 건물중의 2.673 ± 0.026%, MeOH 추출물 중 함량은 25.22 ± 0.25%에 달하였다. 그리고 peroxynitrite 를 소거하는 IC50 값은 5.16 ± 0.15 ㎍/㎖로 나타났고, 양성대조군으로 사용된 L-penicillamine의 값은 0.89 ± 0.22 ㎍/㎖였다. 그러므로, 단양쑥부쟁이는 caffeoylquinic acid 함량에 따르는 peroxynitrite 소거효과에 기인하여 비만, 당뇨, 심혈관질환, 동맥경화 등에 유효할 것으로 예상된다.

원예공로상

임상철 한국원예학회 2010 한국원예학회 학술발표요지 Vol.2010 No.10

북한농업 : 북한농업정책의 특성과 남북농업교류·협력의 전망

임상철 북한연구소 2001 北韓 Vol.- No.355

Laryngopharyngoscope를 이용한 후두개곡낭종의 치료

임상철,조재식 대한기관식도과학회 2000 大韓氣管食道科學會誌 Vol.6 No.2

Cystic lesion which is located in vallecular area is sometimes symptomatic and need surgey. But surgical route to this area is divided external approach into internal one. Internal approach via transoral route with suspension laryngoscope can avoid disadvantages of external approach but conventional laryngoscope is not suitable for this type of procedure. We used Steiner laryngopha-ryngoscope in surgery for vallecular cyst and we could excise cyst easily. Of 20 cases, complete excision was tried in 18 cases and marsupialization in 2 cases. Recurrence was 1 case. We suggest that surgery using laryngopharyngoscope is effective in treating cyst which is located in vallecular area.

강원도 새 농어촌 건설운동: 중국의 농촌운동에 주는 시사점

임상철 사단법인 한국평화연구학회 2010 평화학연구 Vol.11 No.4

Three urgent tasks challenging human beings are global warming, exhaustion of fossil resources, and shortage in food. The evacuation of rural communities, the retrogression of rural industries, and the deterioration of farmers' statuses are matters of great urgency both in Korean and China. This study analyzes the background and process of the Korean Kangwon Province's Rural Community Renovation (RCR) Campaign. China, which is carrying forward the 'Socialist New Rural Community Construction Campaign', would receive some lessons from the Korean precedent. The RCR Campaign is a bottom-up but grass-roots participating movement. It aims at promoting practicability, self-empowerment, and free competition, and spirit, income, and environment are the ideas for fulfilling these objectives. This campaign was so successful that rural communities have revived their vitality. There have been significant progresses in residents' consciousness, village environment, and rural income. However, this campaign was not completely free of short-comings. Chinese rural campaign may refer the Korean experience for its success. Firstly, the incentive fund to the villages of outstanding performance was not properly managed because it was provided as a one-off base. Secondly, the evaluation focused on performances has exacerbated the income inequality of villages. The villages, which have poor resources, have been disadvantaged in this evaluation system. Lastly, it was learned that inter-village networks are pivotal in order to boost the synergy effects of concerned programs. 21세기 인류는 기후온난화, 화석자원의 고갈, 식량부족이라는 3대 당면과제에 접해 있다. 농촌의 공동화, 농업의 쇠퇴화, 농민의 지위저하라는 3농 문제는 한국과 중국 모두 해결해야 될 중차대한 문제이다. 본 논문은 강원도가 성공적으로 추진하고 있는 '새 농어촌건설운동'의 추진배경과 과정을 소개하여 중국의 '사회주의 신농촌건설운동' 추진에 참고자료를 제공하고자 한다. 강원도가 1998년부터 추진하여 온 새농어촌건설운동의 3대 목표는 실사구시, 자력갱생, 자율경쟁이며 3대 이념은 정신, 소득, 환경으로 구성되어 있으며 주민 참여형 하향식 농촌운동이다. 1999년부터 2009년까지 235개 우수마을을 선정하였으며 본 운동의 결과 주민의 의식개혁과 마을의 환경이 획기적으로 개선되고 농가 소득이 향상되어 농촌에 활력을 되찾아가고 있어서 성공적인 농촌운동으로 평가되고 있다. 본 운동의 추진 과정 중에 다음과 같은 요인들이 미흡하였던 점이며 향후 중국의 '신농촌건설운동'에 참고자료가 될 것이다. 첫째, 우수마을에 지급되는 사업비를 일회성으로 지급하기 때문에 우수마을 선정이후 지속적인 관리가 미약하였다. 둘째, 동일한 평가기준에 의하여 심사마을을 일률적으로 평가하는 것은 평가의 객관성을 유지하기는 하지만 우수마을 선정만을 위하여 평가지표 위주의 형식적 업적 쌓기에 치중하는 경우에는 본질적인 사업 목적이 왜곡되는 경우가 발생되게 된다. 셋째, 사업의 시너지 효과를 지속적으로 거양하기 위해서는 마을간 네트워킹 활성화가 무엇보다 중요하기 때문에 마을간 인적 네트워킹이 구축될 필요성이 있다.

외측경부낭종을 동반한 갑상선유두상암종 1례

임상철,박호영,최정섭 대한기관식도과학회 1996 大韓氣管食道科學會誌 Vol.2 No.2

Cystic lesions of the neck are frequently encountered on clinical basis. The usual differential diagnosis includes branchial cleft, cystic hygroma, dermoid cyst, tuberculous lymphadenitis, benign detached goiterous thyroid cyst, and Cavitation epidermoid carcinoma. Most of cysts are benign and malignant cysts are rare. The most common form of cystic malignancy in the lateral neck is cystic degeneration of epidermoid carcinoma metastatic to cervical lymph nodes. Cystic neoplasia may result from malingnant changes occurring within the wall of a previously benign cyst Complete head and neck examination on the primary focus is important Especially, when palpation of thyroid is negative, thyroid carcinoma is easily overlooked. Recently, we experienced papillary carcinoma of the thyroid seen as lateral neck cyst. So we report this case with review of literatures.

술전색전술과 경상악법을 이용한 비인강 혈관섬유종의 치료

임상철 대한기관식도과학회 1996 大韓氣管食道科學會誌 Vol.2 No.1

Nasopharyngeal angiofibroma is rare highly vascular tumor and occurs almost exclusively in adolescent boy. This tumor is histologically benign but clinically malignant because of massive bleeding, destruction of surrounding tissue, difficulty in surgical access and recurrence. Preoperative embolization is required to decrease bleeding during operation Surgical method varies according to staging of angiofibroma. Recently, we had experienced a case of angiofibroma that was resected by transmaxillary approach after preoperative embolization.

기관절개 및 비내시경을 이용하여 치험한 기관이물 1례

임상철,조재식 대한기관식도과학회 1996 大韓氣管食道科學會誌 Vol.2 No.2

Most of bronchial foreign bodies can be removed by ventilation bronchoscopy through transoral route but sometimes, ventilation bronchoscopy through tracheostomy is helpful procedure. Recently, we have experienced a case of bronchial foreign body which could be easily removed by nasal endoscopy and Blakesley forcep instead of bronchoscopy. So we report this case with a review of literatures.

기관 및 기관지를 침범한 재발성다발연골염 1례

임상철,장일권,임성철,박경옥 대한기관식도과학회 1998 大韓氣管食道科學會誌 Vol.4 No.1

Relapsing polychondritis is a rare disesase involving any cartilaginous structure of entire body and is characterized by recurrent episode of inflammation and degeneration of cartilage and most commonly involve ear, nose, larynx, trachea, ribs, Eustachian tube, etc. Its signs and symptoms are recurrent swelling of auricle, saddle nose deformity, polyarthralgia, hoarseness and dyspnea, audiovestibular disturbance and cardiovascular abnormality, etc. Characteristic histologic findings are loss of normal basophilic staining of cartilage, perichondrial inflammatory infiltration with plamsa cells, lymphocytes and neutrophils, and finality, destruction of cartilage and replacement with scar tissue. Our case had saddle nose deformity, arthralgia, tracheal collapse, hearig loss and positive histologic finding but no auricular perichnodritis. Her major problem was airway. obstruction due to tracheal collapse. This case was diagnosed with relapsing polychondritis according to the Damiani's criteria. This case indicates that any patients complaining of airway obstruction have to be examined systemically.

황금 추출물이 CCl₄ 중독 쥐의 간장기능 회복에 미치는 영향

임상철 한국자원식물학회 2010 한국자원식물학회지 Vol.23 No.1

황금추출물의 간장기능 회복능을 알아보기 위하여, CCl₄로 간중독을 유도한 쥐에게 황금추출물을 급여한 후, 혈액성상, 혈액 내 효소활성치 및 간장 내 전염증성 cytokines의 농도변화를 검토했다. RBC, Hb 및 PCV의 값은 처리군 간에 유의한 차이를 나타내지 않았다. 백혈구 총수는 대조군과 비교하여 황금 처리군 모두가 낮은 수치를 나타내었으며, 황금추출물 첨가량이 증가함에 따라 neutrophils 및 eosinophile의 비율이 감소하고, lymphocytes 및 monocytes의 비율이 증가하였다. basophils의 구성비는 처리군 별 유의한 차이를 나타내지 않았다. Total protein 농도 및 albumin 농도는 처리군 간에 유의한 차이를 나타내지 않았다. 그러나 albumin/globulin 농도 비율은 대조군보다 황금추출물 처리군에서 높았다. GOT, GPT 및 LDH의 효소활성치들은 모두가 대조군보다 황금추출물 처리군에서 낮은 수치를 나타내었으며, 황금추출물 첨가량이 증가함에 따라 하락하는 경향을 보였다. 간장 내 IL-1β, IL-6 및 TNF-α의 농도는 대조군보다 황금추출물 처리군 모두가 낮은 값을 나타내었으며, IL-10의 농도는 대조군보다 황금추출물 처리군 모두가 높은 값을 나타내었다. 이상의 결과를 종합해 보면 황금추출물은 간장기능회복에 효과를 나타내는 기능성 물질을 내재하고 있음을 시사해 준다. The objective of present study was to investigate the effect of Baikal Skullcap (Scutellaria baicalensis Georgi) extracts on recovery of liver function in CCl₄-exposed rats. The values of RBC, Hb and PCV did not show significant difference among all treatment groups. The counts of WBC was lower in Skullcap extracts groups than in control group. The ratio of neutrophils and eosinophiles were decreased, and the ratio of lymphocytes and monocytes were increased with increased administration of Skullcap extracts dosage. The ratio of basophils was, however, not significantly different among all treatment groups. The concentration of plasma total protein and albumin showed no significant difference among all treatment groups. The ratio of albumin/globulin was higher in Skullcap extracts groups than in control group. The activities of GOT, GPT and LDH were lower in Skullcap extracts groups, compared to control group. The liver IL-1β, IL-6 and TNF-α concentration were decreased, and IL-10 was increased in Skullcap extract groups, compared to control group. Results of this study suggested that Skullcap may alleviate liver inflammatory reaction induced by liver toxicity.