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CJ-50002(비브리오백신)의 랫드 및 비글개에서의 단회투여 독성시험
이성학(Sung Hak Lee),임동문(Dong Moon Lim),김달현(Dal Hyun Kim),정상보(Sang Bo Jung),박완제(Wan Je Park),이영수(Young Soo Lee) 한국독성학회 1999 Toxicological Research Vol.15 No.2
The single dose toxicity test of CJ-50002, an oral Vibrio vaccine against Vibrio vulnificus injection, was performed in Sprague Dawley (SD) rats and beagle dogs. CJ-50002 was orally administered up to 500 mg/kg in SD rats and 167 mg/kg in beagle dogs. In these experiments, there were no death and clinical changes which were related to CJ-50002. In addition, there were no significant changes between control group and treated groups in body weights and autopsy findings. In conclusion, oral LD50 of CJ-50002 is over 500 mg/kg in SD rats and over 167 mg/kg in beagle dogs.
CJ-50001 (rG-CSF) 의 골수이식모델 마우스에 대한 호중구수 회복 촉진효과
김제학(Je Hak Kim),김현수(Hyun Su Kim),김달현(Dal Hyun Kim),임동문(Dong Moon Lim),조효진(Hyo Jin Cho),김종호(Jong Ho Kim),고형곤(Hyung Kon Ko) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.4
The peripheral neutrophil recovery test was conducted to determine the efficacy of CJ-50001, a drug developed in Cheil Jedang R&D center as a recombinant granulocyte-colony stimulating factor (rG-CSF). Grasin was used as control drug. CJ-50001 and Grasin were subcutaneously administered to γ-ray irradiated mice for 21 days at a dose of 10 ㎍/kg after bone marrow transplantation and the recovery of neutrophil number was examined on the days of 9, 13, 17, and 21 after the drug administration. It was observed that the peripheral neutrophil number of the vehicle control group was recovered to the normal level on the day of 13 after the transplantation whereas the group administered with CJ-50001 and Grasin respectively, showed the normal level of peripheral neutrophil number on 9th day after the bone marrow transplantation. The number of peripheral neutrophils reached the highest level on the 21st day of drug administration, and was recovered to the normal level on the 4th day after ceasing of the drug administration (on the 25th day of the transplantation). Thus, it was presumed that CJ-50001 showed efficacy similar to Grasin on the peripheral neutrophil recovery after bone marrow transplantation.
정상 ICR mouse 및 SD rat 에서 CJ-50001 (rG-CSF) 의 단회투여후 말초호중구수의 변동 및 용량상관성
김제학(Je Hak Kim),김현수(Hyun Su Kim),김달현(Dal Hyun Kim),임동문(Dong Moon Lim),조효진(Hyo Jin Cho),이현수(Hyun Soo Lee) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.4
CJ-50001 is a recombinant granulocyte-colony stimulating factor (rG-CSF) developed by Cheil Jedang R&D Center. The effects of CJ-50001 on the increase of peripheral neutrophil count following intravenous and subcutaneous single administration at a dose of 20 ㎍/kg in normal ICR mice and SD rats, respectively, were compared with those of Grasin, a control drug. Both CJ-50001 and Grasin significantly increased the peripheral neutrophil number in four treatment groups and the maximum number of neutrophil was achieved at 12 to 18 h in rats and mice, respectively. The dose dependency test was studied for CJ-50001 only in normal mice by intravenous or subcutaneous administration. When administered i.v or s.c at the various doses in normal mice, CJ-50001 significantly increased the neutrophil number over the dose of 160 ng/kg, compared with the vehicle control group. From these results, it was concluded that CJ-50001 showed efficacy similar to Grasin in the peripheral neutrophil count increase.
새로운 유전자 재조합 기술에 의하여 생산된 Erythropoietin 의 일반약리작용
김현수(Hyun Su Kim),박관하(Kwan Ha Park),김달현(Dal Hyun Kim),이동억(Dong Eok Lee),김영훈(Young Hoon Kim),정성목(Seong Mok Jeong),임동문(Dong Moon Lim),조효진(Hyo Jin Cho),정재경(Jae Kyung Jung) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.4
The general pharmacological properties of EPO were investigated in various animals administering intravenously and in vitro system. The results were as follows. 1. Central nervous system: EPO at doses of 70, 700, 7000 U/kg showed no effect in mice on general behavior, on strychnine- an ntetrazol-induced convulsion and on acetic acid-induced writhing syndrome. The hexobarbital-induced sle g time in mice was slightly reduced by EPO at a dose of 7000 U/kg but did not change at doses of 70, 700 U/kg. The body temperature in rats was slightly decreased by EPO at doses of 700, 7,000 U/kg but the change was in normal physiological range. 2. Respiratory and cardiovascular system: EPO showed no effect on respiratory rate, blood pressure, heart rate, femoral blood flow, and electrocardiogram in anesthetized dogs at doses of 70, 700, 7000 U/kg. 3. Smooth muscle: EPO at concentrations of 70, 700 U/ml had no effect on the contractile response of isolated guinea pig ileum to histamine and acetylcholine. 4. Water and electrolytes excretion: EPO at dose above 700 U/kg increased urine volume in rats but did not affect the concentrations of Na^+, K^+, Cl^- in urine. 5. Gastrointestinal system: EPO(70, 700, 7000 U/kg) had no effect on the intestinal charcoal meal propulsion in mice. 6. Blood coagulation system: The administration of EPO(70, 700, 7000 U/kg) had no effect on the plasma prothrombin time(PT) and activated partial thromboplastin time(APTT) in mice. Platelet aggregation induced by ADP and collagen was not influenced by EPO(70 U/ml, 700 U/ml). The overall results obtained indicated that EPO exerts almost no serious pharmacological effect even at a 100-fold clinical dose(7000 U/kg).
재조합 사람 과립구 콜로니 자극인자인 CJ50001 의 중합체의 생물학적 활성과 급성독성에 관한 연구
하석훈(Suk Hoon Ha),이현수(Hyun Soo Lee),김기완(Ki Wan Kim),정종상(Jong Sang Sang),김달현(Dal Hyun Kim),임동문(Dong Moon Lim),김종호(Jong Ho Kim),조효진(Hyo Jin Cho),고형곤(Hyung Kon Ko) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.1
CJ50001 is a recombinant human granulocyte colony-stimulating factor (rHuG-CSF) that stimulates the formation of neutrophils from bone marrow stem cells. It was produced in E. coli and purified through refolding and several processes. We produced CS970125(300) using purified CJS0001 and additives in order to test the stability of CJ50001. When CS970125(300) was stored at 50℃ for more than 1 week, high molecular weight proteins were formed and those proteins were detected by non-reducing SDS-PAGE, gel filtration HPLC, and Western blot. Those proteins showed single band at the same position of CJ50001 in reducing SDS-PAGE. These data indicated that those high molecular weight proteins were the multimers of CJ50001. In biological assays, in vitro and in vivo, the multimers did not have biological activity and inhibitory action to that of CJ50001. The mutimers did not induce toxicity in mice and rats in acute toxicity test. These results suggest that if CS970125(300) containing CJ50001 is stored at 50℃, CJ50001 will be the multimers that do not have biological activity and inhibitory effect to CJ50001 and do not induce acute toxicity.