소에 있어서 비외과적 방법에 의한 수정란의 채란 기술개발에 관한 연구

임경순,이용빈,정구민 ( K . S . Im,Y . B . Lee,K . M . Chung ) 한국축산학회 1983 한국축산학회지 Vol.25 No.3

These experiments were conducted to develop embryo collection by nonsurgical methods and. establish basis of embryo transfer in bovine. The recovery rate of embryos between natural and superovulated cows, the ovarian response following 3 to 4 times repeated uses of 2000 IU PMSG with some intervals, cleavage stage of embryo and expansion of uterine cervix with laminaria were studied with korean native cows. The results obtained were as follows: 1. Laminaria No. 1 was expanded into 3.2 times in diameter within 3 hrs and 4 times in diameter within 4 hrs when it was set in warm water at 38℃ and uterine cervix, respectively. After removing it from uterine cervix, foley catheters (18-22FR) were easily inserted into uterus without rectal manipulation. 2. Average numbers of corpus lutea on 9-12 days following estrus induced with 2000 IU PMSG in I, II, III and IV replications taking for 60±15, 90±15 and 150 days intervals among replications were 6.0, 10.3, 7.3 and 7.0, respectively. Accordingly, appropriate interval between replications is thought to be 3 to 4 months and can be shorter or extended according to variation of estrus cycle in the individual. 3. Ovarian response following PMSG injection was greater in fall than in summer and winter. 4. The cows showed standing estrus at average 71±2 hrs following PMSG injection and 85% of the cows showed the estrus between 3 to 4 days following PMSG injection. 5. 92.6% of the flushing fluid was recovered by flushing and 24 eggs (34.3%) among 70 corpus lutea were collected from the superovulated cows. 6. The recovery rates of embryos from I. II, III and IV replications were 50, 3.6, 8.6 and 100%, respectively. The shorter the interval between replications was, the lower the recovery rate of embryo was. 7. Six embryos (100%) were collected from the six cows in natural ovulation by nonsurgical method. 8. Cleavage stages of embryos recovered respectively on 5,7,8,10 and 15 days following estrus ware 16-cell, morula, tight morula, early hatching blastocyst and late hatching blastocyst, respectively. 9. Abnormal, non-fertilized and broken ova among 30 ova recovered were 3(10%), 2(6.7%) and 2(6.7%), respectively. 10. In case of cows in natural ovultion, embryo was not recovered in 1S fractions obtained only by flushing, but mostly recovered in 2nd to 5th factions obtained by flushing with massage of uterus. 11. The embryo recovered on the same day following estrus by PMSG showed 1½±½ difference in cleavage stage between Holstein and Korean native cows and also showed 1±½ difference between the natural and superovulated cows.

돈정액의 동결보존에 관한 연구 1 . 보존액의 조성및 동결조건이 융해후 돈정자의 생존성에 미치는 영향

임경순,정장용 ( K . S . Im,J . Y . Chung ) 한국축산학회 1978 한국축산학회지 Vol.20 No.6

In this experiment effects of straw-size, glycerol equilibration time, thawing temperature, centrifugation of semen, kinds of diluent and addition of ethylene diaminetetraacetic acid 2 Na (E.D.T.A), egg yolk, skim milk and sugar on motility and livability of frozen boar semen were studied. The results obtained from this studies were as follows: 1. The highest post-thawing livability was obtained from the diluted semen exposed to glycerol for 4 hours and from the semen thawed at 40℃ rather than at 10℃. 2. The boar semen in 0.5㎖ straw showed higher post-thawing livability than the semen in 1㎖ straw. 3. 0.2 percent of E.D.T.A and 10 to 20% of egg yolk in the diluent showed higher post-thawing livability between 10 and 20% of egg yolk in diluent. 4. The basic egg yolk tris buffer was modified in this experiment as follows: Trisarminomethane 3.078g, citric acid 1.780g, glucose 0.3g, fructose 0.3g, Glycine 0.48g, catalase 0.02g, E.D.T.A. (Na₂) 0.2g and egg yolk 20㎖ were adjusted to 100㎖ distilled water. One mg of streptomycin arid 1,000 I.U. of penicillin per ㎖ were added to this diluent. 5. The boar semen in skim milk diluent without glycine showed higher post-thawing livability than the semen in skim milk with glycine. 6. The highest post-thawing livability was obtained from the semen diluted with diluent containing 5 to 7% of skim milk and 6% of glucose. 7. The semen diluted with skim milk extender containing 3% of glucose and fructose respectively showed highest post-thawing livability. 8. The basic skim milk diluent was modified in this experiment as follows: skim milk 5g, glucose 3g, and fructose 3g were adjusted to 100㎖ distilled water.

비닐하우스 농작업자의 피로도와 주관적 신체증상에 관한 연구

임경순(Gyung-Soon Lim),김정남(Chung-Nam Kim) 한국농촌의학 지역보건학회 2003 농촌의학·지역보건 Vol.28 No.2

토끼 할구 융합배의 H-Y 항체에 의한 성감별에 관한 연구 1 . 초기배 절단에 의한 자토 생산

임경순(K . S . Im),최화식(H . S . Choi),정구민(K . M . Chung),양보석(B . S . Yang) 한국축산학회 1992 한국축산학회지 Vol.34 No.3

These experiments were carried out to examine development capacity of bisected blastomere by a simple hand method without micromanipulator from 4-cell to morula stage in rabbit. The results obtained from these experiments are as follows: 1) When rabbit embryos were bisected by hand method, both halves undamaged rate was 37.5(3/8). 65.5(33/52) and 65.2(43/66) in 4-8, 16-cell and morula stage, respectively. 2) When demi embryos were cultured, the percentage of bisected embryos developed into blastocysts was 75, 117 and 123% in 4-8, 16-cell and morula stage. respectively 3) When 30 demi-embryos from 16-cell and 70 demi-embryos from morula were transferred into 3 and 7 recipients, 3/30(10) and 6/70(8.9%) young obtained, respectively.

발정혈청 및 양수가 소 난포란의 체외성숙에 미치는 영향

임경순(K . S . Im),김형선(H . S . Kim),정구민(G . M . Chung),박연진(Y . J . Park),김현종(H . J . Kim),박광욱(K . W . Park) 한국축산학회 1993 한국축산학회지 Vol.35 No.3

These experiments were carried out to investigate the effect of addition of estrous cow serum(ECS), amniotic fluid(AF) and AF + FCS on maturation of bovine oocytes in vitro. The oocytes with cumulus cells and unfragmented cytoplasm were used. The oocytes were matured in vitro for 24 hr in a CO₂ incubator with 5% CO₂ in air at 38.5℃. The medium used for maturation was TCM 199 and Ham`s F10 supplemented with hormones and antibiotics. The results obtained are as follows : I. When the oocytes were cultured in TCM (99 medium, maturation rate of oocyte in FCS 10%. ECS 5, 10, 15 and 20% was 70.0, 74.4, 75.0, 78.1 and 58.8%, respectively. There were no significant (p$gt;0.05) difference among the treatments. 2. When the oocytes were cultured in Ham`s F10, maturation rate of oocyte in AF 0, 10, 50 and 100% was 63.0, 87.3, 63.6 and 7.8%, respectively. AF 10% showed significantly (p$lt;0.05) higher maturation rate than others. 3. When the oocytes were cultured in Ham`s F10, maturation rate of oocyte FCS + AF showed significantly (p$lt;0.05) higher maturation rate(76.9%) than FCS only (50.8%).

남북한 보건의료제도의 비교

임경순(Lim, Gyung Soon),김정남(Kim, Chung Nam),박경민(Park, Kyung Min) 한국보건간호학회 2001 韓國保健看護學會誌 Vol.15 No.1

혈장단백질이 돼지동결정자의 생존성 , 두모형태 및 수태에 미치는 영향

정용균,임경순,이용빈 ( Y . K . Chung,K . S . Im,Y . B . Lee ) 한국축산학회 1986 한국축산학회지 Vol.28 No.2

This experiment was carried out to investigate the effects of plasma proteins on the sperm survival index, acrosome morphology and fertilizing capacity of frozen-thawed boar semen. Diluted semen with modified Beltsville F_5(mBF5) containing 0.05% of Porcine Albumin (PA) was frozen into pellets of 0.10 ㎖ on Dry Ice. The pellets were transferred to liquid nitrogen for storage. For each insemination 10 ㎖ of pellets were removed from liquid nitrogen. The pellets were dumped into 100-㎖ beaker containing 45 ㎖ of modified Beltsville Thawing Solution (mBTS) at 37℃ and thawed. Each sow was inseminated twice with 8×10^9 spermatozoa per dose. The results obtained from this studies were as follows: 1. In the diluted semen with mBF_5 and BF_5 extender, the addition level of 0.05 and 0.20 percent of plasma proteins did not show any difference in the sperm survival index after first and second dilution. 2. Sperm survival index of frozen thawed semen containing 0.05% of PA was significantly higher (p$lt;0.01) than addition of any other plasma proteins in BTS and mBTS. In addition of 0.20% level of PA, sperm survival index of frozen thawed semen was significantly higher (P$lt;0.05) than any other treatments in mBTS, but was not show significant differences in BTS. 3. The percentage of normal acrosome was 88 to 94% in fresh semen of boars, but there were no significant differences among the Yorkshire, Hampshire, Duroc and Landrace. 4. In BTS, the percentage of normal acrosome of frozen thawed semen containing 0.05% or PA was significantly higher (P$lt;0.05) than any other treatments, but in addition of 0.20% level of plasma proteins did not show significant differences. 5. In mBTS, the percentages of normal acrosome of frozen thawed semen containing 0.05% of PA and BSA were significantly higher (P$lt;0.01) than any other treatments, also addition of 0.20% of PA and BSA were significantly (P$lt;0.05) different. And addition of 0.05% level of EA was significantly higher (P$lt;0.05) than PG, BG and C. 6. The percentage of normal acrosome of frozen semen thawed in mBTS was show significantly higher (P$lt;0.05) than in B`TS. 7. Pregnancy with semen frozen in mBFs containing 0.05% of pA and thawed in mBTS was 6/12 (50%) and the average litter size was 7.8 pigs.

돈정액의 동결보존에 관한 연구 1 . 동결융해가 정자두모의 형태 및 수태에 미치는 영향

정장용,임경순 ( J . Y . Chung,K . S . Im ) 한국축산학회 1979 한국축산학회지 Vol.21 No.4

In order to obtain high sperm livability after thawing of frozen boar semen, the different combinations of 1st and 2nd dilution with egg yolk tris-dilutor (E), skim milk dilutor (S) and E+S (1 : 1) were studied, Acrosome system of pre and post-frozen spermatozoa in the dilators of different combination was also studied Fertility of frozen semen in the dilators of different combination was tested. The results obtained from these studies were as follows 1. The semen in first dilator with E+S and second dilator with S showed highest post-thawing livability among the treatments. 2. The semen in 1st dilator with E+S and 2nd dilator with S showed highest livability among four diluents during 7 hrs storage at room temperature after thawing. 3, Sixty to 70% of spermatozoa were injured in acrosomic system especially as a slight injury by freezing of semen. This injury of acrsomic system was somewhat inhibited by 1st dilution with EMS and 2nd dilution with S. 4. None of the two sows inseminated with frozen semen in 1st dilutor with E and 2nd dilutor with E was pregnant, but one of the four sows inseminated with frozen semen in 1st dilutor with E+S and 2nd dilutor with S was pregnant and produced five litters with two female and three male pigs, and another three were not pregnant.

소의 품종 , 배양액의 첨가제 및 발정주기가 난포란의 체외성숙 , 수정 및 발생에 미치는 영향

정구민(K . M . Chung),임경순(K . S . Im) 한국축산학회 1988 한국축산학회지 Vol.30 No.2

This experiment was conducted to investigate the effects of breeds, medium supplements and ovarian phases on the developmental pattern of bovine follicular oocytes. Ham`s F10 powder was dissolved in the 3rd demineralized and 5th distilled water and supplemented with 10% heat inactivated fetal bovine serum (HI-FBS) or 0.4% bovine serum albumin (BSA). The medium was equihlmated for 16±2 hours in 5% CO₂ incubator and adjusted in pH 7.4-7,6 and in 280±10 mOsm. The oocytes were recovered from small antral follicles (2 to 5㎜) and matured for 24 to 26 hours at 39℃. Matured oocytes were fertilized with ejaculated sperm (1-2 x 10^6/㎖) and cultured for 4 days at 38.5℃ in 5% CO₂ in air. The cleavage rate of oocytes was higher in Korean native cattle (KNC; 22.0%, 18/82) than ins Holsteins (4.4%, 4/93). Eleven among eighty two (13.2%) of KNC oocytes developed to morula stage, but none of Holstein oocytes cleaved up to 8 cells. Of the developed ova, rate of embryos with regular blastomeres was also higher in KNC (∼65%) than in Holsteins (∼33%). Holstein oocytes were fertilized with KNC sperm to clear the reason why the developmental potential of Holstein oocytes was extremely limited. Their cleavage rate was 16.7%o (4/24) and was higher than that of Holstein oocytes fertilized with Holstein sperm. Therefore the low potential in vitro of Holstein oocytes was due to oocytes and not to sperm. Cleavage rate of KNC oocytes was higher in FBS supplemented medium (22.0%, 18/82) than in BSA supplemented medium (14.8%, 4/27). The oocytesdeveloped to morula stage in FBS-medium, but non-cleaved up to 3 cells in B SA-medium. Of the developed ova, rate of embryos with regular blastomeres is higher in FBS-medium (∼65%) than in BSA-medium (∼33%). Of the uncleaved 1-cell ova, rate of fragmental ova was significantly higher in BSA-medium (33.3%) than in FBS-medium (14.6%). By contrast, the rate of deformed ova was significantly lower in BSA-medium (7.4%) than in FBS-medium (25.0%). The cleavage rate of oocytes was not different between luteal phase and follicular phase, but the rate of embryos with regular blastomeres was higher in luteal phase than in follicular phase. In conclusion, the rates of cleavage and normal development of follicular oocytes were higher in KNC than in Holsteins, in FBS-medium than in BSA-medium, and in luteal phase than in follicular phase. The cleavage rate of oocytes was related to the rate of normal development. Immature-follicular oocytes of KNC can normally mature and develop to morula stage in Ham`s F10 supplemented with 10% FBS but that of Holsteins can not.

미성숙 및 성숙미경산우의 다배란 처리가 난소반응 , 수정란 생산과 수태에 미치는 영향

김종국(J . G . Kim),정구민(K . M . Chung),임경순(K . S . Im),이용빈(Y . B . Lee) 한국축산학회 1988 한국축산학회지 Vol.30 No.5

This experiment was carried out to investigate the effects of superovulation on the ovarian response and non-surgical recovery in immature and mature heifers. The possibility of nonsurgical recovery from the immature heifers and pragnancy of embryos transferred to mature recipients was also investigated. Total 8 heifers (S heads of Holstein, 2 heads Korean Native Cattle (KNC) and 1 head of Angus × Hereford crossbred) were devided into 2 groups by their age. Immature heifers (Group I) were 7-10 months old and mature heifers (Group II) were 12-18 months old. Heifers of Group I and Group II were superovulated with muscular injection of 1,000 or 2,000 IU Pregnant Mare`s Serum Gonadotropin (PMSG) on day 10-12 of estrous cycle followed by muscular injection of 25㎎ PGF₂α 48h later. Heifers were artifically inseminated or copulated 2 to 3 times with the interval of 12h while they were in heat. The embryos were collected non-surgically on day 6 of estrous cycle. The results obtained from this study were summarized as follows: 1. The average number of corpus lutea (CL) by the superovulation treatment was much greater in Group II (9.2) than in Group I (3.2). 2. The average number of unruptured follicles by superovulation treatment was much grater in Group II (6.8) than Group I (1.0). 3. The interval to onset estrus after PGF₂α injection was longer in Group I (44.2) than in Group II (31.8h). 4. There was no difference in the duration of estrus between Group I (32.0h) and Group II (37.5h). 5. It was possible to flush the uterus of 7 month old KNC heifers non-surgically. 6. The average number of embryos recovered was much greater in Group II (6.0) than in Group I (2.3) and the recovery rate of embryos in Group I (73.6%) was similar to that Group II (87.3%). More flushing fluid was recovered in Group I (93.0%) than in Group II (87.3%). 7. Twelve of 14 embryos recovered (87.5%) were normal in Group I, while 17 of 54 embryos (31.5%) were normal in Group II. 8. All of the normal embryos (12/12) were morula in Group I, while 82.4% (14/17) of the normal embryos were monala and 17.6%o (3/17) were blastocysts in Gooup II. 9. The estrous cycle after superovulation treatment tended to become longer with the average of 24.8 days in Group I and 24 days in Group II. Some donor heifers had cystic ovaries. 10. Six embryos were transferred to 4 recipients non-surgically. One recipient who had received 2 morula that were recovered from a 10 month old KNC-2 became pregnant and delivered a male Korean native calf of 27 Kg.