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RISS 인기검색어
- 검색키워드
- 저자 : 이 죤스태바(E. John Staba) (검색결과 4 건)
- 무료
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인삼식물에 관한 연구 (Ⅰ) - 미국인삼 사포닌 및 그 비당체
김정연(Jung Yun Kim),이 죤스태바(E. John Staba) 한국생약학회 1973 생약학회지 Vol.4 No.4
The saponins of two- and four-year-old American ginseng plants (Panax quinquefolium L.) (Araliaceae) collected in July and September were studied. American ginseng saponins (panaquilins) differ from Korean ginseng (Panax ginseng C.A. MEYER) saponins (ginsenosides). The American ginseng saponins separated and named were panaquilins A, B, C, D, E-1, E-2, E-3, G-1, G-2, (c) and (d). One-dimensional thin-layer chromatography did not completely separate panaquilin mixture and was subject to misinterpretation. The panaquilins were more accurately separated and identified by the two-dimensional thin-layer method established. Some differences in American ginseng saponins were dependent upon the plant age, time of collection, and part extracted. The American ginseng sapogenin components are panaxadiol (panaquilins B and C), oleanolic acid (panaquilin D) and panaxatriol (panaquilin G-1). The panaquilins E-1, E-2 and E-3 mixture contained both panaxadiol and panaxatriol. The genins of panaquilins A, (c), (d) and G-2 were not identified. In addition, β-sitosterol and stigmasterol were identified from the root ether extracts.
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Studies on the Ginseng Plants (Ⅲ) Radioactive Sodium Acetate-U-C<sup>14</sup> Feeding Experiments
Jung Yun Kim(김정연),E. John Staba(이 죤스태바) 한국생약학회 1974 생약학회지 Vol.5 No.2
The radioactive compound sodium acetate-U-C<sup>14</sup> (C<sup>14</sup>-acetate) was administered to two- and four-year-old July and September American ginseng (Araliaceae, Panax quinquefolium L.) plants and cuttings. The C<sup>14</sup>-acetate uptake was approximately 99 %. The autoradiochromatograms suggest that the saponins isolated by preparative thin-layer chromatography contained impurities, especially those isolated from the leaf and stem extracts. The root and fruit methanol extracts yielded relatively pure saponins. The large amounts of panaquilin B and its proximity to panaquilin C on preparative thin-layer plates resulted in some admixing. The average concentration (% plant dry weight) of semi-purified saponins were high in the leaves (13.8 %), as compared to fruits (9.8 ,%). stems (7.9 %) and roots (6.3 %). The average percentage of C<sup>14</sup>-acetate incorporation into panaquilins was 4. 8 %. The average percentage of C<sup>14</sup>-acetate incorporation into panaquilins B and C was higher (1.40 % and 1.13 %, respectively) than that into panaquilins C, (d), G-1 and G-2 (0.75 %, 0.65 %, 0.13 % and 0.53 %, respectively). Panaquilin synthesis may be depending upon the part, collection period and age of the plant. The average percentage of C<sup>14</sup>-acetate incorporation into panaquilin B is high in roots (0.58 %) and stems (0.48 %); that into panaquilins C and (d) high in leaves (0.40 % and 0.45 %, respectively) ; and that into panaquilin E high in roots and leaves (0.55 % and 0.50 %, respectively). Panaquilin G-2 was synthesized in all parts of plants. The panaquilins appear to be biosynthesized more actively in July than September (exception-panaquilin G-1). Panaquilins B, C and G-1 may lie biosynthesized more actively in four-year-old plants ,and panaquilins (d) and E more actively in two-year-old plants. The results from expectance with , cuttings suggest that the panaquilins are synthesized de novo in the above-ground parts of ginseng plants, and that panaquilin G-1 may be synthesized de novo in the leaf. It is known from the tissue culture studies that panaquilins are produced by leaf, stem and root callus tissues and callus-root cultures of American and Korean ginseng plants. Panaquilins may actively be synthesized de novo in most any cell or organ of the ginseng plants. It was verified that C<sup>14</sup>-acetate was incorporated into the panaxadiol portions of the panaquilins of two-year-old plants (sp. act. 0.56 mμCi/㎎) and four-year-old plants (sp. act. 0.54 mμCi/㎎).
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