유색 찰옥수수의 항산화력 및 작물학적 특성

이희봉,박보영,지희정,조진웅,김석형,모은경,이미라,Lee, Hee-Bong,Park, Bo-Young,Ji, Hee-Chung,Cho, Jin-Woong,Kim, Seog-Hyung,Mo, Eun-Kyung,Lee, Mi-Ra 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

본 시험 결과를 요약하면 다음과 같다. 1. 공시교잡종의 평균 과피두께는 $37{\mu}m$로 얇은 특성을 보였는데 특히 CNU108 교잡종이 $30.3{\mu}m$로 가장 얇은 특성을 보였고 출사일수는 CNU69와 CNU202 등이 59일로 가장 빨랐으나 CNU160 교잡종은 68일로 본 교잡종들 중에서 가장 늦었다. 2. CNU70과 CNU138의 교잡종들은 높은 항산화효소(xanthin oxidase, catalase, superoxide dismutase)의 활성을 나타내었고, 특히 CNU160과 CNU193 교잡종은 각각 94.8%, 94.6% 등으로 높은 항산화력(EDA)을 나타내었다. 3. CNU109와 CNU34의 교잡종에서는 전반적으로 높은 항산화효소 활성 및 항산화력을 나타내었다. 따라서 CNU34, CNU70, CNU108, CNU138, CNU193 등의 교잡종 찰옥수수가 항산화 효과가 높은 고기능성 유색 찰옥수수인 것으로 사료되었다. This study was carried out to evaluate major agronomical characterization and phenol compound contents, Xanthine oxidase inhibitory activity (XO), Catalase activity, Superoxide dismutase activity (SOD) and 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical sacvenging activity were analyzed in colored waxy corns. The mean of stem height and ear length were 248.8 cm and 18.6 cm, respectively. The pericarp thickness in CNU108 $(30.3{\mu}m)$ was thinner than other hybrids. The period of tasseling days in CNU69 and CNU202 were very shorter than other hybrids (59 days). 100-kernel weight of CNU50 was 35.6 g and heavier than the others. The antioxidant activities such as xanthin oxidase (XO), catalase and superoxide dismutase (SOD) were higher in CNU70 and CNUI 38. Especially highest EDA (electron donating ability) in DPPH radical scavenging effect was 94.8% and 94.6% in CNU160 and CNU193, respectively. In the results, the antioxidant enzyme activity and antioxidant acticity were higher in CNU109 and CNU 34 hybrids. The hybrids, CNU34, CNU70, CNU108, CNU 138 and CNU193 may be considered higher functional color waxy corn.

Metabolism of Ginseng Saponin in Animal Body (I)

이희봉,주충노,Lee, Hee-Bong,Joo, Chung-No 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.2

인삼(Panax ginseng C.A. Meyer) 뿌리 절편을 효소원으로 사용하여 $^{14}C$-acetate로부터 제조한 방사성 인삼 사포닌을 Thin layer chromatography, High performance liquid chromatography, Autoradiography 등으로 확인하였다. 1 mg의 인삼 사포닌을 쥐에게 구경 투여하고 1시간 후, 간장으로부터의 추출물을 분석한 결과, 회수된 방사능의 약 25%가 분해되지 않은 사포닌 상태로 존재하고 있음을 확인하였다. 또한 인삼 사포닌 투여 (구경)후 시간에 따른 방사능의 변화 양상을 초사한 결과 간에서의 사포닌의 반감기는 4~6시간, 인삼 사포닌의 농도는 $10^{-5}%$ 수준으로 예측되었다. $^{14}C$-labelled ginseng saponins from $^{14}C$-acetate were prepared using the root slices of panaxginseng C.A. Meyer as an enzyme source and the products were demonstrated to be identical with the saponins extracted from the roots by thin layer chromatography, high performace liquid chromatography and autoradiography. The radioactivity of the liver was monitored on time course after the feeding of 1 mg of ginseng saponin containing $^{14}C$-labelled saponin. The extract from the liver of rats killed at one hr. after the oral administration of 1 mg of ginseng saponins containing the $^{14}C$-saponins was analyzed found that 25% of the radioactivity recovered was found as undissociated form of the saponin. It semed that the half life of the saponin might be 4~6 hrs and the concentration of the saponin would be $10^{-5}%$ level in the liver.

Incorporation of Foreign Gene with Ti Plasmid Vector System: (II) Expression of E. coli Thioredoxin Gene in Cultured Tobacco Cells.

이희봉,주충노,홍순주,김성완,임창진,김영명,Lee, Hee-Bong,Joo, Chung-No,Hong, Soon-Joo,Kim, Seong-Wan,Lim, Chang-Jin,Kim, Young-Myeong 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.4

본 연구에서는 E. coli thioredoxin 유전자를 함유하고 있는 재조합된 plasmid pKDB3의 담배세포내로의 도입을 시도하고, 도입된 thioredoxin 유전자의 발현을 조사하였다. 담배(Nicotina tabacum cv Xanthi) 세포로의 재조합 plasmid pKDB3의 도입은 담배잎 절편과 재조합 DNA 및 helper Ti plasmid pTiBo 542를 함유하고 있는 Agrobacterium tumefaciens strain A281과의 cocultivation 방법을 이용하여 행하여졌으며, 형질전환된 담배세포는 항생물질 저항성 배지에서의 callus 형성 여부로 선별되었고, 선별된 형질전환된 calli는 shoot와 root 형성을 위해 적절한 MS agar 배지에서 계속 키워졌다. 이와 같이 형질전환된 담배세포에서 완전한 식물체로 재생된 담배잎에서의 E. coli thioredoxin 유전자의 발현을 조사한 결과 thioredoxin 활성이 형질전환된 담배세포가 정상세포에 비해 9배 정도 더 큰 것으로 나타났다. 이러한 일련의 결과들은 E. coli thioredoxin 유전자가 성공적으로 담배세포에 들어가서 높은 수준으로 발현됨을 보여주고 있다. This study was performed to observe the expression of E. coli thioredoxin gene incorporated in tobacco cells. The recombinant DNA used, pKDB3, had been constructed from a Ti plasmid vector pGA658 and a bacterial plasmid pCJF4 harboring E. coli thioredoxin gene, as described in the preceding paper (Lee et al., 1988). The leaf discs of plant (N. tabacum cv Xanthi) were transformed to kanamycin resistance by the cocultivation with Agrobacterium A281 containing plasmid pKDB3. Transformed leaf discs were cultured in MS agar medium with kanamycin for callus induction. Kanamycin-resistant tobacco calli were continuously grown in MS agar medium for shoot induction, and then in MS agar medium for root induction. Expression of E. coli thioredoxin gene in the plant tissue regenerated from transformed tobacco cells was confirmed by DTNB assay. The thioredoxin activity of transformed tobacco cells was much higher (about 9 times) than that of normal tobacco cells. Our results suggest that E. coli thioredoxin gene was successfully incorporated into tobacco cells, and the incorporated bacterial gene could be expressed at a high level.

탑의 원조 인도 스투파의 형태 해석 - 인도 전역의 현장 답사를 바탕으로 -

이희봉,Lee, Hee-Bong 한국건축역사학회 2009 건축역사연구 Vol.18 No.6

This study aims to discover historical trends and change of form of all stupas in India with observation of field study that is as direct as possible, by classifying, analyzing, and synthesizing the stupas. Study of Indian stupa in Korea has a number of shortcomings since only introductory partial approach has been made in order to seek the origin of Korean pagoda. This study also aims to correct errors of stupa terminology in Chinese character committed by misinterpretation of Hindi language which was established by precedent Japanese scholars several decades ago. Piled-up stupas were totally destroyed by pagans, therefore their remains tell us only of structure, material, sizeand disposition. However remains of carved stone at torana and drum give us clues as to the original form of stupa and worshipping activity, as well as change to a more luxurious form. Many rock cave stupas of India show us both simple forms matching the ascetic age of early Buddhism and luxurious changes in Mahayanan era introducing us to statues of Buddha. Indians recovered the spheric form of 'anda,' a Hindi term meaning cosmic egg, from the hemispheric form of the piled-up stupa. Therefore we might discard the erratic term of 'bokbal', which means an upset vessel. Railings and parasols became main factors of stupa design. Carved railings around stupa became a sign of divinity. Serious worshipping activity made drums long or high and created multi-embossed stripes. Bases of circular drums of some cave stupas changed their shapes to rectangular or octagonal. Single parasols became multiparasols of affluent flowerlike curved stems on carved stupa. Multistoried, elongated and high parasols of Gandhara stupas are closely related to such factors as diverse changes of form in Indian subcontinent. Four-sided torana gate and ayaka column of the circular form of original stupas suggest the rectangular form of subsequent East Asian pagoda, and higher and wider base of Indian stupas became the origin of East Asian rectangular pagoda.

Ti plasmid vector 를 이용한 진핵세포 유전자의 도입에 관한 연구

이희봉,주충노,홍순주,김성완,임창진,김영명 ( Hee Bong Lee,Chung No Joo,Sun Joo Hong,Seong Wan Kim,Chang Jin Lim,Young Myeong Kim ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.1

DNA recombination using plasmid pGA658, a Ti plasmid vector, was tried to study the role of yeast homoserine dehydrogenase after introducing its gene into plant cells. Two recombinant plasmids with opposite orientation of the gene DNA under nos promoter of vector pGA658, pKDB1 and pKDB2, were constructed by the aid of two intermediate vectors, pUC7 and pUC119. Transformed E. coli and Agrobacterium tumefaciens were confirmed by the resistance to appropriate antibiotics and by sizing DNA restriction fragments after plasmid isolation. Assay of homoserine dehydrogenase activity showed that a little increase in its activity was detected in transformed E. coli. but no increase in transformed Agrobacterium tumefaciens, compared with its untransformed strain.

Ti Plamid Vector System 을 이용한 외래 유전자의 도입 : ( 2 ) E . coli thioredoxin 유전자의 배양된 담배세포내 발현

이희봉,주충노,홍순주,김성완,임창진,김영명 ( Hee Bong Lee,Chung No Joo,Soon Joo Hong,Seong Wan Kim,Chang Jin Lim,Young Myeong Kim ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4

This study was performed to observe the expression of E. coli thioredoxin gene incorporated in tobacco cells. The recombinant DNA used, pKDB3, had been constructed from a Ti plasmid vector pGA658 and a bacterial plasmid pCJF4 harboring E. coli thioredoxin gene, as described in the preceding paper (Lee et al., 1988). The leaf discs of plant (N. tabacum cv Xanthi) were transformed to kanamycin resistance by the cocultivation with Agrobacterium A281 containing plasmid pKDB3. Transformed leaf discs were cultured in MS agar medium with kanamycin for callus induction. Kanamycin-resistant tobacco calli were continuously grown in MS agar medium for shoot induction, and then in MS agar medium for root induction. Expression of E. coli thioredoxin gene in the plant tissue regenerated from transformed tobacco cells was confirmed by DTNB assay. The thioredoxin activity of transformed tobacco cells was much higher (about 9 times) than that of normal tobacco cells. Our results suggest that E. coli thioredoxin gene was successfully incorporated into tobacco cells, and the incorporated bacterial gene could be expressed at a high level.

Ti Plasmid Vector System 을 이용한 외래 유전자의 도입 : ( 1 ) A . tumefaciens 로의 E . coli Thioredonxin 유전자의 도입

이희봉,주충노,홍순주,김성완,임창진,김영명 ( Hee Bong Lee,Chung No Joo,Soon Joo Hong Seong Wan Kim,Chang Jin Lim,Young Myeong Kim ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4

In this part of study on the incorporation of foreign gene into plant cells, a derivative of Ti plasmid vector (pGA658), containing E. coli thioredoxin gene, was prepared and introduced into Agrobacterium tumefaciens. A recombinant plasmid, pKDB3, was constructed by transferring HindIII-BamHI DNA fragment of pCJF4, including E. coli thioredoxin gene, into HindIII-BgIII restriction sites of plasmid pGA658. By doing this, E. coli thioredoxin gene is expected to express from nos promoter of pGA658 after the incorporation into plant cells. The structure of DNAs isolated from kanamycin-resistant E. coli transformants was convinced by restriction mapping. As a preceding step before incorporation into plant cells, the recombinant plasmid pKDB3 was transformed into A. tumefaciens by freeze-thaw procedure. In Agrobacterium transformants, the expression of E. coli thioredoxin gene was positively observed, and this suggested the stable existence of the E. coli gene.

인삼 사포닌의 체내대사 ( Ⅰ )

이희봉,주충노 ( Hee Bong Lee,Chung No Joo ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.2

^(14)C-labelled ginseng saponins from ^(14)C-acetate were prepared using the root slices of panaxginseng C.A. Meyer as an enzyme source and the products were demonstrated to be identical with the saponins extracted from the roots by thin layer chromat ography, high performace liquid chromatography and autoradiography. The radioactivity of the liver was monitored on time course after the feeding of 1 ㎎ of ginseng saponin containing ^(14)-C-labelled saponin. The extract from the liver of rats killed at one hr. after the oral administration of 1 ㎎ of ginseng saponins containing the ^(14)C-saponins was analyzed found that 25 % of the radioactivity recovered was found as undissociated form of the saponin. It semed that the half life of the saponin might be 4∼6 hrs and the concentration of the saponin would be 10^(-5) % level in the liver.

상류(上流) 전통주거(傳統住居) 해남(海南) 녹우당(綠雨堂)의 해석(解釋) -거주자(居住者) 생활(生活)과 농업경영(農業經營)으로-

이희봉,이향미,Lee, Hee-Bong,Lee, Hyang-Mee 한국건축역사학회 2002 건축역사연구 Vol.11 No.1

This study has main purpose to understand and interpret the house Nok-U-dang, an upper class built in Chosun dynasty, not by outsider researcher's view but insider dweller's view. To interpret correctly, dweller's everyday life in the space and form is examined on a microscale beyond the physical space and form of the house, main object of architectural history To understand the present form exactly, the study restore traditional life in past era, 1940s. Main method of restoration is the ethnographic interview, based on cultural anthropology. Like any other upper-class house, the house has been influenced under ruling Confucian ideology in Chosun dynasty: separation of man's and woman's quarters and hierachical arrangement by generation, and worshipping ceremony for ancestor. However, it is by practical management for agricultural production that every court and building of the Noku-Dang can be explained correctly; preparing seed for sowing, tool storing, preparing and serving meal for laborers, making manure, harvesting, threshing grain, storing grain and so on. Precedent studies interpreted the house by the Confucian principle too much and made conclusion of dignity and austerity of ritual: woman's quarter, is closed and serene space. However this study shows that the space is semi-opened and composite space by agricultural works. And the Sarangchae, master's quarter, is located properly at visual center to control every agricultural activity.

Call Admission Control using Soft QoS-based Borrowing Scheme in DVB-RCS Networks

이희봉,장영민,이호진,Lee, Hee-Bong,Jang, Yeong-Min,Lee, Ho-Jin The Korean Institute of Communications and Informa 2005 韓國通信學會論文誌 Vol.30 No.2a

본 논문에서는 DVB-RCS (Digital Video Broadcast-Return Channel via Satellite) 망에서 호 수락제어 (CAC)를 위한 Soft-QoS 기반의 borrowing 기법을 제안한다. 제안된 기법은 위성 망에서 사용 가능한 자원이 부족할 때 새로운 호를 받아들이기 위해 서비스 중인 호 중 일부에서 일시적이고 공평하게 대역폭을 가져온다. 각각의 호에서 가져오는 대역폭의 양은 각 사용자의 critical bandwidth ratio에 비례하며 critical bandwidth ratio는 제안된 기법에서 이용하는 QoS 파라메타 중 하나이다. 시뮬레이션 결과는 제안된 기법이 호의 blocking 확률과 대역폭 효율 측면에서 성능의 향상이 있음을 보여준다. We propose a soft QoS-based borrowing scheme for call admission control(CAC) in DVB-RCS(Digital Video Broadcast-Return Channel via Satellite). Some of the ongoing calls temporarily and fairly release bandwidths that can be used to accommodate a new call in an overloaded satellite network. The amount of bandwidth borrowed from each call is proportional to each user's critical bandwidth ratio, one of parameters for soft QoS mechanism. Simulation results show that the proposed scheme improves the system performance in terms of call blocking probability and bandwidth utilization.