흰쥐 편도체의 Dopamine 수용체에 대한 전자현미경 자기방사법적 연구

이희래 梨花女子大學校 醫科大學 醫科學硏究所 1989 EMJ (Ewha medical journal) Vol.12 No.1

The present study was performed to identify the dopaminergic receptor sites in rat amygdala. Identification of the receptor sites at ultrastructrual level has been done by autoradiography after cerebral ventricular perfusion of ^3H-haloperidol. From 434 examples of silver grains, 5% were labeled in soma, 36% in thick dendrites, 26% in the thin branches of axons and dendrites, 21% in thick axons and terminals and 12% in synapses with synaptic vesicles. In the cases of labeled synapses, all of axon terminals contained small round vesicles and showed asymmetric contact with dendrites. By these results it may be implied that dopamine receptors in the amygdala are located in both pre-and post-synaptic sites of the asymmetric axo-dendritic synapses with small round vesicles and the axons of labeled synapses originated from the dopaminergic neurons in the midbrain.

췌관결찰때의 집토끼 Paneth 세포에 대한 전자현미경적 연구

이희래,권흥식 대한해부학회 1975 Anatomy & Cell Biology Vol.8 No.2

성장기 흰쥐 시각피질의 신경연접에 대한 도은법 및 전자현미경적 연구

이희래,Lee, Hee-Lai 한국현미경학회 1997 Applied microscopy Vol.27 No.4

These studies were performed to observe the morphological changes of synapses in the visual cortex of rat during early postnatal development. Specimens of the visual cortex were taken from rats (Sprague Dawley) at 1, 3, 7, 14 and 21 days of age, and prepared for silver impregnation and electron microscopy. The number of synapse and the length of postsynaptic thickening were increased progressively with age, especially 14 and 21 days. The number of dendritic spine was increased conspicuously on postnatal days 14-21. And asymmetic, curved and axo-spinous synapses were increased markedly at the same ages. The present findings suggest that spurt of synaptogenesis in the rat visual cortex occurs during early postnatal development, especially in second to 3rd week period and asymmetric and/or curved axo-spinous synapse is a matured form of synapse with advanced age.

흰쥐 腦 被殼내의 Dopamine 軸索終末에 대한 自記放射法的 硏究

李熙來 梨花女子大學校 韓國生活科學硏究院 1985 韓國生活科學硏究院 論叢 Vol.36 No.-

본 실험은 피각에서 dopamine 수용체와 관련있는 축삭종말을 구분하기 위하여 시도하였다. 건강한 흰쥐에 ^3H-spiroperidol을 체중 kg당 1mCi씩 미정맥에 주사하였다. 동물은 1% paraformaldehyde-2% glutaraldehyde액으로 관류고정하고 피각조직을 절취하여 2% osmium tetroxide에 후고정하였다. 조직의 초박절편은 Ilford L_4 emulsion을 입혔고 4˚C에서 12주간 노출시킨 다음 phenion developer로 현상하고 uranyl acetate와 lead citrate로 염색하여 관찰하였다. 피각의 조직에서 은입자는 축삭종말과 수상돌기에 많이 표지되었는데 특히 소형원형연접소포를 가진 축삭종말과 이들과 연접을 형성하는 수상돌기에서 높은 표지율을 나타내었다. 이러한 사실에 의하여 피각내에 존재하는 소형원형연접소포를 가진 축삭종말의 일부는 흑질-선조체로의 dopamine 신경세포에서 기원된 것이라고 생각한다. This study was performed to identify the dopaminergic axon terminals in rat putamen. In adult Sprague-Dawley rats 1mCi/kg body weight ^3H-spiroperidol as a dopamine receptor blocking agent was injected into the tail vein. For electron microscopic autoradiography, the ultrathin sections were coated with Ilford L_4, emulsion and were developed by phenidon developer. From 439 examples of silver grains 55% were located in the axon terminals, 39% in dendrites, 4% in myelinated nerve fibers and the remaining in soma. From ^3H-spiroperidol labeled grains which associated with axon terminals, about 95% were located in the axon terminal with small round vesicles. By these results it may be implied that a lot of the axon terminals with small round vesicles in the putamen originate from the dopaminergic neuron of substantia nigra.

膽囊切除 토끼에서 輸膽管 分泌異常에 依한 十二指腸 上皮細胞의 形態學的 變化

李熙來,金淳會 梨花女子大學校 韓國生活科學硏究院 1985 韓國生活科學硏究院 論叢 Vol.35 No.-

체중 2kg내외의 수토끼를 사용하여 이들을 정상군과 담낭절제군으로 구분하였고 담낭절제군은 수술 후 3주 및 7주에 희생시켰다. 정상군과 담낭절제군의 동물은 ether 마취하에 개복하고 일정한 부위의 십이지장 조직을 절취하였다. 광학현미경적 관찰은 10% 중성 formalin액에 고정, hematoxyline-eosin과 periodc acid Schiff's 반응-hematoxyline 염색을 시행한 표본으로서, 전자현미경적 관찰은 2.5% glutaraldehyde-2% paraformaldehyde으로 전고정하고 1% osmium tetroxide에 후고정하여 epon 812에 포매, uranyl acetate와 lead citrate로 염색한 표본으로 관찰한 결과 다음과 같은 소견을 얻었다. 1. 담낭 절제 후 십이지장 근위부에 분포하는 흡수세포는 첨부 세포질내에 음소포가 증가하였으며 측벽의 세포간극은 확장되었다. 2. 십이지장 근위부의 배상세포는 핵 상부 세포질에 점액질 과립이 증가하였고 과립의 배출상태도 많이 관찰되었다. 위와 같은 현상들은 담낭 절제로 인하여 담즙이 계속 유출됨으로서 흡수세포는 흡수기능이 항진되고 배상세포는 분비활동이 촉진되어 나타난 결과라고 생각된다. Effects of cholecystectomy on the functional activities of the duodenal mucus secreting cells and columnar absorptive cells were studied with healthy male rabbits, normal or cholecystectomized, each weighing about 2kg. Animals were sacrificed on the 3rd or 7th week after cholecystectomy. Tissue specimens of duodenum, embedded in paraffin or in epon, were examined by light and electron microscope, respectively. We observed that the number of pinocytotic vesicles increased in the apical cytoplasm of absorptive cells and intercellular spaces of absorptive cells became dilated in the proximal region goblet cells were found to contain large amount of mucus globules and to increase in globular discharging activity. These observations indicated that functional activities of the absorptive cells and goblet cells might be accelerated in the proximal part of duodenum by cholecystectomy.

출생후 성장과정의 흰쥐 새줄무늬체에서 신경연접의 구조적 변동

이희래,Lee, Hee-Lai 한국현미경학회 1995 Applied microscopy Vol.25 No.4

This study was performed to analyze the morphological changes of synapses during early postnatal periods. Neonatal rats were grouped by 3, 7, 14, 21, 28 and 42day old, and observed the ultrastructural pattern of the synapses in the neostriatum by transmission electron microscope. 1. The number of synapse, the length of postsynaptic thickening and the amount of synaptic vesicles markedly increase during postnatal development 2. The proportion of asymmetric and curved synapses gradually increase by developmental periods. From the above results, it is suggested that the size of synapse increase during post-natal period, and asymmetric synapse are formed from the symmetric type and curved synapse are formed from the plane type.

기니픽 뇌 미상핵의 신경세포 및 신경연접에 대한 전자현미경적 연구

이희래 梨花女子大學校 醫科大學 醫科學硏究所 1986 EMJ (Ewha medical journal) Vol.9 No.1

The caudate nucleus of the guinea pig was examined at the ultrastructural level. At least four types of neurons and seven types of synapses are distinguished. The large polygonal neurons are measured 18-25㎛ in diameter and have large amount of cytoplasm with many stacks of granular endoplasmic reticulum, concentrations of ribosomes, and rich Golgi cisternae. The medium-sized, ovoid neurons are the most frequent cells. These neurons are measured 10-15㎛ in diameter and contain a lot of organelles. The medium-sized, polygonal neurons are measured 11-18㎛ in diameter. These cells have cytoplasm with moderate amount of granular endoplasmic reticulum and Golgi cisternae. The small neurons are the least common never cells in the caudate nucleus. They have a nucleus with indentations, little Golgi apparatus and few cisternae of granular endoplasmic reticulum. The axon terminals containing small round vesicles are the most frequent type of synapses. The majority of them make asymmetric contact on dendritic spines of neurons. Some of remainders make symmetric contact with dendrites and somata and others asymmetric contact with the same structures. Few of terminals 'en passant' with small round vesicles contact on dendrites and somata. The terminals containing elongated vesicles contact symmetrically on the dendrits and somata. All of the terminals containing pleomorphic or large round vesicles make synapses in the same manner of elongated vesicle terminal.

원숭이 腦 靑斑의 電子顯微鏡的 觀察

李熙來 梨花女子大學校 韓國生活科學硏究院 1982 韓國生活科學硏究院 論叢 Vol.29 No.-

본 실험은 청반내에 분포하는 신경세포의 미세구조를 관찰하기 위하여 시도되었다. 실험동물인 다람쥐원숭이를 nembutal 마취하에 고정액인 1% paraformaldehyde와 2% glutaraldehyde(phosphate buffer pH 7.4)로 전고정하고, 2% osmiumtetroxide(phosphate buffer pH 7.4)로 후고정하여 Epon 812에 포매한 후 전자현미경 표본을 작성하여 관찰한 바 청반의 신경세포는 중형세포와 소형세포로 구분되고, 중형세포는 다시 축삭세포와 체연접의 수에 따라서 연접이 많은 세포와 적은 세포로 구분되며, 일부 축삭세포체연접은 세포체극에서 형성된다. The neuronal cell bodies of the locus coeruleus ofthe monkey (Saimiri sciureus) were investigated using electron microscopy. On the basis of size and shape of cell body, branching pattern of dendrites, distribution of cytoplasmic organelles and number of axosomatic synapses the locus coeruleus was observed three types of neuronal perikarya; medium-sized polygonal, medium-sized ovoid and small neurons. The medium-sized polygonal neurons were polygonal shapes measured to be 10~26㎛ in diameter. These neurons had thick dendrites with numerous branchings and small number of axosomatic synapses. The medium-sized ovoid neurons were ovoid or spherical shapes measured to be 13~23㎛ in diameter. All neurons of this type were characterized by a high number of axosomatic synapses. The small neurons(9~21㎛) displayed pale cytoplasm containing few organelles, Most of the axosomatic synapses make a direct contact to the soma but some of them do by somatic appendages.

절식상태 하에서 마우스 Paneth Cell의 형태학적 반응

이희래,권흥식 대한해부학회 1974 Anatomy & Cell Biology Vol.7 No.1

원숭이 뇌 청반 신경연접의 전자현미경적 관찰

이희래,김순회 梨花女子大學校 醫科大學 醫科學硏究所 1982 EMJ (Ewha medical journal) Vol.5 No.3

This study provides a description of the normal morphology of the synapses of the locus coeruleus in the squirrel monkey, Saimiri sciureus, at the electron microscopic level. The animals were perfused with 2% glutaraldehyde-1% paraformaldehyde fixative in phosphate buffer at pH 7.4. Tissues from the locus coeruleus were postfixed by 2% osmium tetroxide in phosphate buffer at pH 7.4, and dehydrated in graded ethanol and aceton, and embedded in Epon 812. The polymerization was carried out in oven at 60℃. The ultrathin sections were cut on a LKB ultratome, contrasted with uranyl acetate and lead citrate, and examined in a Siemens Elmiskop 1A electron microscope. Special attention during the observation was given to the different types of synapses. Five types of synapses with many characteristics, could be differentiated : small-round vesicle terminals(52%), pleomorphic vesicle terminals(25%), elongated vesicle terminals(16%), small-round and dense core vesicle terminals(5%) and large-round vesicle terminals(2%). Some of the axosomatic synapses with small round or pleomorphic vesicles make an indirect contact to the soma by somatic appendages.