신증후 출혈열환자의 Immunoglobulin 형성에 관한 연구

이호왕,성인화,오인석,주용규 대한바이러스학회 1987 Journal of Bacteriology and Virology Vol.17 No.1

This experiment investigated 5 classes of immunoglobulins to Hantaan virus in patients of Hemorrhagic fever with renal syndrome from immediately after onset of the disease to 2 months. The results obtained from this experiment were as follows: 1. The highest titer of IgG antibody to Hantaan virus could be demonstrated and reached to a peak between 2nd and 3rd week from onset of fever. Most of the patients showed high titers of immunofluorescent antibody between 4,096 and 16,384. 2. IgA antibody to Hantaan virus in patients of Hemorrhagic fever with renal syndrome was relatively high and reached a peak between 2nd and 3rd week from onset of the disease, and it was maintained with high titer of antibody for 2 months. Peak titers of immunofluorescent antibody were between 1,024 and 4,096. 3. IgM antibody to Hantaan virus in the patients of Hemmorrhagic fever with renal syndrome was demonstrated in all of the patients after onset of the disease and the titers of the antibody were between 256 and 4,096 for 2 months. 4. Though IgD antibody in patients of Hemorrhagic fever with renal syndrome was reached to a peak between 2nd and 3rd weeks after onset of the disease, the titers of the antibody were within 1,024 and it was demonstrated for 2 months. 5. IgE antibody in the patients of Hemorrhagic fever with renal syndrome was irregularly appeared and the increase of antibody was demonstrated in half of the patients. Above results revealed that the demonstration of IgM could be the important criteria in diagnosis of the primary infection with Hantaan virus and that the significance of increase of IgD antibodies to Hantaan virus dur- ing illness remains to be analysed.

한탄바이러스의 물리화학적 성상 - II . 온도 및 pH에 대하여 -

이호왕,백락주,성인화,곽태로 대한바이러스학회 1983 Journal of Bacteriology and Virology Vol.13 No.1

Korean hemorrhagic fever (KHF) was recognized for the first time in Korea in 1951 during the Korean War when it infected the United Nations troops but similar diseases to KHF had been reported in Russia, China and elsewhere. In 1976, Lee and Lee discovered a specific antigen of KHF and demonstrated that this antigen is the etiologic agent of KHF and perfected a serologic test for diagnosis of the disease in 1978. In 1980 the etiologic agent of KHF was named Hantaan virus after the Hantaan river. Very recently, close etiological relations have been shown by serologic means between KHF in Korea and hemorrhagic nephroso-nephritis in USSR, between KHF and nephropathia epidemica in Scandinavia, between KHF and epidemic hemorrhagic fever (EHF) in Japan and between KHF and EHF in China. The working group on Hemorrhagic fever with renal syndrome at a WHO meeting in Tokyo in February 1982 recommended that the above mentioned diseases with different names should be referred as Hemorrhagic Fever with Renal Syndrome (HFRS). Although there are several reports on the transmission, sensitivities of various animals and electron microscopic findings of Hantaan virus, no report was described on physicochemical properties of the virus. This is the first report on the effect of temperature and pH on the infectivity of Hantaan virus in Apodemus mice. 1. The diameter of Hantaan virus was approximately 90 nm. 2. Hantaan virus was inactivated rapidly at above 37C. The infectivity of virus, however, was slowly decreased but preserved for about one month at 4C. 3. The optimal pH for infectivity of Hantaan virus in Apodemus mice was 7. 6 but it was inactivated completely at below pH 5.0. 4. It was demonstrated that BSS with 0. 2% bovine albumin, pH 7. 2-7. 6, was the most stable diluent for the study of the infectivity of Hantaan virus.

조직배양한 Vero E6 세포에서의 한탄바이러스 및 서울바이러스 분리

이호왕,백락주,김금철,이용주 대한바이러스학회 1990 Journal of Bacteriology and Virology Vol.20 No.1

한국형 (韓國型) 출혈열 (出血熱) - II . 병원체 (病原體) 분리 (分離)

이호왕(李鎬汪),이평우(李平佑) 대한바이러스학회 1977 Journal of Bacteriology and Virology Vol.7 No.1

Epidemic hemorrhagic fever was recognized for the first time in Korea in 1951 and since that time it has been known as Korean hemorrhagic fever (KHF). Similar diseases to KHF have been known in Manchuria, the Soviet Union, Scandinavia, Eurasia and Japan. Many attempts bave been made to isolate the causative agent of KHF and clinically similar diseases but failed. In 1976 Lee and Lee succeeded in demonstrating an antigen in the lungs of the striped field mouse, Apodemus agrarius, which gave immunofluorescent reactions with sera of patients convalescent from KHF for the first time and named it as Korea antigen. Seventy-three strains of the etiologic agent of KHF which reacted specifically only with the convalescent sera of KHF patients were isolated from lung tissues of Apodemus agrarus coreae rodents and also same agents were isolated from acute sera of two patients in adult Apodemus. The agent was successfully propagated in Apodemus through 13 passages. Experimentally inoculated mice developed sp cific fluorescent antigen in lungs, kidneys, liver, parotid glands, bladder. The agent was neutralized with convalescent serum of KHF, chloroform sensitive, passed through 0.1p millipore filter and not sensitive to antibiotics. The results indicate that the agent is the causative agent of KHF and a small virus.

候鳥에서의 日本腦炎 바이러스에 對한 中和抗體證明

이호왕(李鎬汪),강신혁(姜信赫) 대한바이러스학회 1976 Journal of Bacteriology and Virology Vol.6 No.1

Birds were found to be rescrvoir of Japanese encephalitis virus (JEV) by isolation of the virus from birds by studies of Buescher and Scherer et al. It has been thought that birds might have relationship with overwintering mechanisms of JEV and Reeves et al succeeded in inducing artificial latent infection of Western equine encephalitis virus in hirds by inoculation of the virus, There is another hypothesis to explain the every-summer endemicity of Japanese encephalitis in the endemic area of temperate zone, that migrating birds of tropical areas carry the virus to the endemic area every spring or early summer but it was not proved. This experiment was carried out to isolate Japanese encephalitis virus and also to examine the frequencies of JE virus infection in migratory birds. It was found that about 7% of migratory birds collected in springs of Korea contained neutralizing antibodies to JEV but virus isolation was unsuccessful.

실험실 감염의 문제점

이호왕 大韓法醫學會 1985 대한법의학회지 Vol.9 No.1-2

인천시민 및 인천집쥐의 Reovirus에 대한 항체분포

이호왕,성인화,백락주,주용규,김금철 대한바이러스학회 1983 Journal of Bacteriology and Virology Vol.13 No.1

The distribution of immunofluorescent antibodies against Reovirus type 3 in the sera from 566 residents and 155 house rats collected in Incheon in the spring of 1982 was studied and the results are as follows: 1. In human, the positive rates of immunofluorescent antibodies against Reovirus type 3 were evaluated ro be about 79% in the first decade group, 75% in the second decade group, 90% in the third decade group, 93% in the fourth decade group, 97% in the fifth decade group, 99% in the above and sixth decade group and no significant difference in sex. 2. In rats, the positive rates of immunofluorescent antibodies against Reovirus type 3 were shown to be about 67% in R. norvegicus, 20% in R. rattus and no significant difference in sex. This study showed that most of the resideats in Incheon were infected by Reovirus in their childhood and two third of rats in that area were infected by Reovirus.

韓國人의 肝炎B抗原分布 關한 硏究

이호왕(李鎬汪),이용주(李容周),이평우(李平佑),백승용(白承龍),이규진(李圭振) 대한바이러스학회 1974 Journal of Bacteriology and Virology Vol.4 No.1

Acute viral hepatitis remains a rnajor worldwide public health problem. Epide'miologic and laboratory investigations indicate that 2 specific viruses are involved in this disease. The epidemiologic distinctiveness of these 2 viruses has recently been challenged. Viral hepatitis A, transrnitted primarily by the fecal-oral route, is also transmitted by the parenteral route. Viral hepatitis B occurs sporadically following parenteral inoculation of virus-infected blood products, although recent studies have deinonstrated that oral transinission can also occur. Distribution of Hepatitis B antigen (HB Ag) in Korean was investigated and the results are followings. 1. 3.1% out of 7,360 sera from paid blood donors of Korea University Woo-Suk hospital blood bank was HB Ag positive. In early stage of test, it was 5-10% but subsequently decreased gradually and was about 3% at the end of 1974. 2. The distribution of HB Ag in each age groups was investigated. The highest was in 11-20 age group (12. 7%) and 21-40 age group succeeded it. In sexual difference of it, male was twice as high as female in 11-20 age group but the difference was little in other age groups. The positive rate of antibody to HB Ag was the highest in 11-20 age group (2.7%) 3. Of 1,407 sera from out patients of Woo-Suk hospital, the distribution of HB Ag was the highest (9.8%) in the Department Gastrointestinal medicine and next was the Departments Chest surgery (9.5%) and Urology (7.8%) The distribution of hepatitis B antibody was the highest in General surgery (5.2%), Urology (4.7%) and next was Chest internal medicine (1.%) 4. The frequency of HB Ag in hlood donors from three blood banks in Seoul, positiveness was about 7% and out patients were 5.2%.

등줄쥐와 한탄바이러스의 생물학적 관계에 관한 연구

이호왕,백락주,방영남 대한바이러스학회 1984 Journal of Bacteriology and Virology Vol.14 No.1

The etiological agent of Korean hemorrhagic fever (KHF) was first discovered from lung tissue of Apodemus agrarius by Lee and Lee in 1976. In 1978, Lee et al. Had isolated the same agent from KHF patients sera and had succeeded the propagation of the virus in Apodemus agrarius. In 1981, French et al. Succeeded in the propagation of the agent in a tissue culture cell line. In 1981, the etiological agent of KHF was named Hantaan virus after the Hantaan river by Lee. The etiological relations have been shown by serologic means among KHF in Korea, hemorrhagic nephroso-nephritis in USSR, nephropathia epidemica in Scandinavia, epidemic hemorrhagic fever (EHF) in Japan and EHF in China. At a WHO meteing in Tokyo in February 1982 the working group on Hemorrhagic fever with renal syndrome recommended that the above mentioned diseases with different names be referred as Hemorrhagic fever with renal syndrome (HFRS). The natural reservoir of Hantaan virus in Korea was demonstrated as Apodemus agrari coreae. In the report of the transmission of Hantaan virus in Apodemus mice, lage amounts of virus were excreted in urine and saliva. The susceptibility of Apodemus mice to Hantaan virus was analysed by sex, age, location of collection of the mice and route of inoculation. The amount of virus in the urine excreted daily from infected Apodemus mice was calculated. 1. There was no difference in susceptibility of Apodemus mice by sex and age after inoculation of Hantaan virus. 2. The susceptibility of Jejudo mice (Apodemus agrarius jejuensis) and Chindo and Kyungkido mice (Apodemus agrarius coreae) to Hantaan virus was almost equal. 3. The most high infectivity of Hantaan virus among the different route of inoculation was by direct intralung inoculation of virus and infection occurred via intranasal and oral routes also. 4 The order of amount of virus in the organs of infected Apodemus mice by Hantaan virus was as follows; lungs, salivary glands, kidneys and liver. 5 The daily average amount of urine that was excreted by an Apodemus mouse was about 1.8 ml and contained about 20,000-40,000 Apodemus mice ID of Hantaan virus.

한탄바이러스에 대한 면역억제제 및 항혈액응고제의 작용

이호왕,백락주,성인화,이용주,홍유선 대한바이러스학회 1984 Journal of Bacteriology and Virology Vol.14 No.1

ln 1976, Lee and Lee discovered the etiologic agent of Korean hemorrhagic fever (KHF), Hantaan virus, although similar diseases to KHF had been reported as hemorrhagic nephrosonephritis in Russia, as nephropathia epidemica in Scandinavia and epidemic hemorrhagic fever in China. In 1981, French et al. Reported a succesful growth of Hantaan virus in A 549 cells which originated from alveolar epithelial cells of a human lung cancer. The growth of Hantaan virus and the appearance of antigen-antibody complex in lung tissues of A-podemus agrarius following intramuscular inoculation of the virus and cyclophosphoamide (procytox) and also the effect of anticoagulants to infectivity of Hantaan virus in Apodemus agrarius were studied. The following results were obtained. After inoculation of Hantaan virus in Apodemus agrarius, the formation of immune complex was effectively suppressed by consecutive injection of procytox intramusculary per 3-6mg three times at certain intervals. 2. The effect of anticoagulants, EDTA, Heparin and Sod. Citrate to infectivity of Hantaan virus in Apademus agrarius was not demonstrated. Among these, EDTA was the most safe anticoagulant in Hantaan virus experiment.