전복내장추출물의 항산화 및 human dermal fibroblasts에 대한 항피부노화 효과

이정뢰 ( Jing Lei Li ),동도 ( Tao Tong ),고두옥 ( Du Ock Ko ),정동옥 ( Dong Ok Chung ),정원철 ( Won Chul Jeong ),김지은 ( Ji Eun Kim ),강성국 ( Seong Gook Kang ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2012 한국식품저장유통학회지 Vol.19 No.4

In this study, the anti-oxidant and anti-elastase activities of four abalone viscera extracts were investigated to screen the most promising extract. This extract was further studied in terms of its anti-skin-aging properties. In the DPPH-scavenging assay, the Tris-HCl extract showed a 58.60±0.88% radical-scavenging activity, which was followed closely by the ethanol extract that had a 55.40±0.62% scavenging activity. In the anti-elastase assay, however, the ethanol extract showed the significantly highest elastase inhibition activity. Furthermore, none of the extracts had a harmful effect on the human dermal fibroblast, as revealed in the MTT assay. In the cell study, the effect of the ethanol extract at various concentrations on the human dermal fibroblast was investigated. At the 10 μg/mL concentration, the ethanol extract boosted the pro-collagen type I synthesis to 705.30±3.06 ng/mL and reduced the MMP-1 to 54.30±0.80 ng/mL, which was considered the optimum concentration. This is the first study that focused on the anti-oxidant and anti-skin-aging effects of abalone viscera extract. Its results may provide fundamental data for further study.

한국에서 생산되는 전복의영양성분 비교 분석

이정뢰 ( Jing Lei Li ),김보섭 ( Bo Sub Kim ),강성국 ( Seong Gook Kang ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2013 한국식품저장유통학회지 Vol.20 No.4

전복의 기능성과 성분에 대한 연구와 한국 전복의 지역별 성분특성에 대한 연구는 거의 없는 실정이다. 본 연구에서는 한국의 주요 전복 생산지인 완도를 중심으로 남족인 제주도와 북쪽인 흑산도 전복의 육과 내장에 대하여 일반성분, 미네랄, 아미노산, 지상산 및 콜라겐 함량을 비교 분석하였다. 전복내장 중의 수분과 단백질 함량은 전복육에 비하여 낮은 경향을 보였으나 지방과 탄수화물 함량은 더 높았다. 지역간 전복의 일반성분과 미네랄 함량은 다소 차이가 있는 것으로 나타났으며 수온이 낮은 지역일수록 지방함량이 약간 높았다. 전복내장의 구성아미노산 함량으 93.6~127.02 mg/g이었으며 이는 전복육의 평균 145 mg/g에 배해 낮은 편이었다. 반면에 전복내장의 유리아미노산 함량은 16.81~20.10 mg/g으로 전복육의 7.90~10.59 mg/g에 비하여 2배 높은 값을 보였다. 전복육의 총 구성아미노산 지역별 차이는 없으며 전복내장의 결가를 살펴볼 때 완도와 흑산도 높고 제주도 낮은 것을 나타났다. 전복육 총 유리아미노산은 완도 전복은 가장 높았으며 이어서 흑산도와 제주도순이었고 내장의 경우는 완도, 제주도와 흑산도 순으로 총 유리아미노산 함량이 낮아졌다. 전복육의 지방산조성은 불포화지방산이 약 41%로 포화지방산 약 31%에 비하여 높았으며, 전복육보다 전복내장이 높은 결과를 보였다. 콜라겐 함량은 완도 전복육에서 2.26±0.46 mg/g으로 가장 높았으며 잉서 제주도와 흑산도 순이었다. 전복내장에서는 0.20±0.03~0.37±0.15 mg/g으로 전복육보다 낮은 값을 보였다. 본 연구 결과는 향후 전복에 관한 연구에서 유용한 정보로 활용될 수 있을 것으로 기대된다. In this study, the general compositions, amino acids, fatty acids and collagen content of three abalones, which were harvested in Wando, Heuksando and Jcju Island ill Korea, were analyzed and compared. The abalone body and viscera were separated from each other and the physicochemical properties were investigated The moisture and protein contents of the viscera were lower than that of the body; while the lipid, carbohydrate, and mineral contents WCIt~ higher. TIle geneml compositions and mineral contents also presented Ilucnmnon among the three abalone groups. The amino acid content via hydrolysis in the abalone viscera ranged fmm 93.16 to 127.02 mglg. which was lower than the amino acid content of the abalone body of approximately 145 mglg. However, the free amino acids level in the abalone viscera mnged from 16.81 to 20.10 mg!g, which was higher than that of the abalone body level of approximately 7.90 to 10.59 mglg. TIle fatty acid analysis revealed that while tile saturated fatty acid percentages ill the abalone body were higher tlUU1 in the abelore viscera, the percentages of thc moroenoic and potyenolc fatty acids of the body were significantly lower. The body of the ebalore harvested in Wando contained the highest level of collagen (2.26±0.46 mglg), followed by the abalone harvested-in Jeju Island and Heuksaudo. The abalone viscera contained milch tower collagen, which ranged from O.37±O.15 mglg to O.20±O.03 mg/g. TIle results of this sndy will provide useful information 1`01" the future research of abalone. In this study, the general compositions, amino acids, fatty acids and collagen content of three abalones, which were harvested in Wando, Heuksando and Jcju Island ill Korea, were analyzed and compared. The abalone body and viscera were separated from each other and the physicochemical properties were investigated The moisture and protein contents of the viscera were lower than that of the body; while the lipid, carbohydrate, and mineral contents were higher. The general compositions and mineral contents also presented fluctuation among the three abalone groups. The amino acid content via hydrolysis in the abalone viscera ranged from 93.16 to 127.02 mg/g. which was lower than the amino acid content of the abalone body of approximately 145 mg/g. However, the free amino acids level in the abalone viscera ranged from 16.81 to 20.10 mg/g, which was higher than that of the abalone body level of approximately 7.90 to 10.59 mg/g. The fatty acid analysis revealed that while tile saturated fatty acid percentages ill the abalone body were higher tlUU1 in the abelore viscera, the percentages of the monoenoic and polyenoic fatty acids of the body were significantly lower. The body of the abalone harvested in Wando contained the highest level of collagen (2.26±0.46 mg/g), followed by the abalone harvested-in jeju Island and Heuksando. The abalone viscera contained much Iower collagen, which ranged from O.37±O.15 mg/g to O.20±O.03 mg/g. The results of this sndy will provide useful information 1`01" the future research of abalone. In this study, the general compositions, amino acids, fatty acids and collagen content of three abalones, which were harvested in Wando, Heuksando and Jcju Island ill Korea, were analyzed and compared. The abalone body and viscera were separated from each other and the physicochemical properties were investigated The moisture and protein contents of the viscera were lower than that of the body; while the lipid, carbohydrate, and mineral contents WCIt~ higher. TIle geneml compositions and mineral contents also presented Ilucnmnon among the three abalone groups. The amino acid content via hydrolysis in the abalone viscera ranged from 93.16 to 127.02 mglg. which was lower than the amino acid content of the abalone body of approximately 145 mg/g. However, the free amino acids level in the abalone viscera ranged from 16.81 to 20.10 mg/g, which was higher than that of the abalone body level of approximately 7.90 to 10.59 mg/g. The fatty acid analysis revealed that while tile saturated fatty acid percentages ill the abalone body were higher tlUU1 in the abalone viscera, the percentages of the monoenoic and polyenoic fatty acids of the body were significantly lower. The body of the abalone harvested in Wando contained the highest level of collagen (2.26±0.46 mg/g), followed by the abalone harvested-in Jeju Island and Heuksando. The abalone viscera contained milch tower collagen, which ranged from O.37±O.15 mg/g to O.20±O.03 mg/g. The results of this study will provide useful information 1`01" the future research of abalone.

고추장굴비의 품질과 저장성에 미치는 초고압처리와 감마선 조사 효과

강성국 ( Seong Gook Kang ),박난희 ( Nan Hee Park ),고두옥 ( Do Ock Ko ),이정뢰 ( Jing Lei Li ),김보섭 ( Bo Sub Kim ),박양균 ( Yang Kyun Park ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2011 한국식품저장유통학회지 Vol.18 No.1

Kochujang-gulbi, a Korean traditional food, was prepared with kochujang and freeze-dried gulbi slices. Kochujang-gulbi was treated with high hydrostatic pressure (200, 400 and 600 MPa) and gamma-irradiation (7, 10, 20 and 30 KGy) to improve its quality and shelf-life. The pH of high hydrostatic pressure and gamma-irradiation treated kochujang-gulbi samples did not significantly different compared to that of control. However, Hunter L value slightly increased, and Hunter a and b values decreased by high hydrostatic pressure and gamma-irradiation treatment. During storage, the pH and color of high hydrostatic pressure and gamma-irradiation treated kochujang-gulbi samples did not significantly changed. The VBN and TBA level decreased by the increase treatment of high hydrostatic pressure and then slowely increased during storage. The VBN and TBA level of kochujang-gulbi samples treated by the strong gamma ray emmission slowely increased during storage. In particular, gamma-irradiation treatment was very effective to sterilize microorganisms when compared to that of high hydrostatic pressure in kochujang-gulbi products. In addition, the high hydrostatic pressure and gamma-irradiation treated kochujang-gulbi samples shown significantly lower total viable cell number than control for the 20 days of storage at room temperature with retort pouch packaging.