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젖소 동결수정란의 비외과적 이식에 있어서 수정란의 상태 및 이시조건이 수태율에 미치는 영향
이은송,조충호,황우석,Lee, Eun-song,Jo, Choong-ho,Hwang, Woo-suk 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.3
This study was performed to investigate the effects of stage and quality of embryo, synchrony between donor and recipient and difficulty of transfer on pregnancy rate following non-surgical transfer of frozen-thawed bovine embryos. The results were as follows; 1. The overall pregnancy rate of this experiment was 63.4% and that of heifers(73.1%) was higher than that of cows(46.7%). 2. The pregnancy rates of recipients transferred with morulae, early blastocysts and blastocysts were 50.0%, 64.7% and 71. 4%, respectively. 3. The pregnancy rate of recipients transferred with good embryos(67.9%) was higher than that of recipients transferred with fair embryos(53.8%). 4. The pregnancy rates of embryos transferred to left and right uterine horn were 63.2% and 63.6%, respectively. 5. The pregnancy rate of recipients in estrous synchrony 0(76.2%) was higher than those of recipients in synchrony -1(55.6%) and +1(44.4%). 6. The pregnancy rate of recipients transferred with 2 embryos (71. 4%) was higher than that of recipients transferred with 1 embryo(61.8%). 7. The pregnancy rate of embryos transferred to uterine tip (72.0%) was higher than that of embryos transferred to uterine base(50.0%). 8. Ease of transfer was ranked to a scale of one to three on the basis of increasing difficulty. Transfers ranked as ease score 1 accounted for 77.8% of pregnancies and had higher pregnancy rate than ease score 2(66.7%) or 3(45.5%). 9. The pregnancy rate of recipients with excellent corpus luteum(CL) (70.0%) was higher than those of recipients with good CL(61.1%) or fair CL(61.5) %. In reviewing above results, it was considered that the factors such as embryo stage, embryo quality, estrous synchrony, corpus luteum quality, transfer site within uterus, recipient's parity and ease score affected the pregnancy rate after non-surgical transfer of frozen-thawed bovine embryos.
동결액에 첨가된 macromolecule 및 EGF, FGF가 vitrification 법으로 동결한 소 수정란의 체외생존성에 미치는 영향
이은송,후쿠이 유타카,Lee, Eun-song,Fukui, Yutaka 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.2
Cryopreservation of embryos by vitrification is a simple method to preserve bovine embryos for subsequent embryo transfer, but embryonic viability after vitrification has been inconsistent and low compared with conventional slow freezing. The aim of the present study is to examine the effect of serum or serum albumin in a vitrification solution and epidermal growth factor(EGF) or fibroblast growth factor(FGF) on in vitro viability of bovine blastocysts frozen by vitrification. Bovine blastocysts were produced by in vitro maturation, fertilization of follicular oocytes and culture of embryos in a synthetic oviduct fluid medium(SOFM) containing BSA and 19 essential and nonessential amino acids. Blastocysts with excellent or good morphology were selected at 7 or 8 days after culture and utilized for vitrification. In experiment 1, blastocysts were vitrified in a solution containing semi-fetal calf serum(SFCS) or BSA(5 or 10mg/ml) and then their subsequent viabilities were examined by culturing thawed embryos in a SOFM containing BSA and 19 amino acids. Effect of EGF or FGF added to a SOFM containing polyvinyl alcohol(PVA) on the viability of vitrified-thawed blastocysts was investigated in experiment 2. BSA added at 5 or 10mg/ml to a vitrification solution showed significantly higher(p < 0.05) developmental rate to expanded and hatching blastocysts than SFCS, but there was no significant difference in the developmental rate to hatched blastocysts after thawing. Supplementation of a culture medium with EGF and/or FGF significantly increased(p < 0.05) embryo development to expanded blastocysts compared with control but showed no beneficial effect on the development to hatching or hatched blastocysts. Coculture of thawed embryos with granulosa cells in a TCM 199 containing 10% fetal calf serum(FCS) showed the highest developmental rate to expanded, hatching and hatched blastocysts among the groups tested. In conclusion, supplementation of a vitrification solution with BSA at 5mg/ml and culture of thawed blastocysts in a medium containing EGF and/or FGF can improve in vitro viability of bovine blastocysts frozen by vitrification.
이은송 ( Eun-song Lee ),백승기 ( Seung-ki Back ),성진호 ( Jin-ho Sung ),김정훈 ( Jeong-hun Kim ),이주찬 ( Joo-chan Lee ),조수진 ( Soo-jin Cho ),( Ahm Mojammal ),서용칠 ( Yong-chill ) 한국폐기물자원순환학회(구 한국폐기물학회) 2018 한국폐기물자원순환학회 추계학술발표논문집 Vol.2018 No.-
수은의 배출로부터 국민의 건강과 환경을 보호하기 위하여 국제수은협약(Minamata Convention on Mercury)이 2013년도에 채택되다. Article 11에서는 수은폐기물을 수은 오염, 함유, 구성 폐기물 등 총 3가지 종류로 구분하여 정의하고 있다. 국내 폐기물관리법에 따라 수은 폐기물은 별도 분류되고 있지 않으나, 지정폐기물로 간주되어 안정화 또는 고형화 처리 후 관리형 매립지에 최종 처분하도록 지정하고 있다. 본 연구에서는 국내 산업시설로부터 배출된 비산재 등을 대상으로 킬레이트를 이용한 안정화 처리기술에 대한 연구를 진행하였다. 대표적인 킬레이트 물질로 알려진 EDTA 0.1 mol과 염화수은 (HgCl<sub>2</sub>) 등을 안정한 형태인 황화수은 (HgS)으로 전환시키기 위해 황이 포함된 황화나트륨 (Na<sub>2</sub>S) 0.2 mol, 비산재 10g을 6시간동안 혼합하였다. 안정화 처리 후 고체와 액체를 분리시키기 위해 105 ℃에서 충분히 건조시켜 수분을 증발시켰다. 이후 킬레이트 처리된 비산재의 화학적 안정도를 평가하기 위하여 pH에 따라 5단계 용출단계로 구성된 단계별용출법을 적용하였다. 각 단계별 용매로써 증류수(1단계), 0.1M CH<sub>3</sub>COOH+0.01M HCl(2단계), 1M KOH(3단계), 12M HNO3(4단계) 및 Aqua regia(5단계)를 사용하였다. F1 단계에서 3%, F단계 약 10%, F3~5단계에서는 80~90%의 수은이 용출되었다. 이러한 사실로 보아 염화수은 (HgCl<sub>2</sub>) 등 용해도가 높은 수은화학종이 화학적으로 안정한 형태로 전환되었다고 추측된다. 본 반응의 화학적 매커니즘을 파악하기 위하여 XRD 분석을 적용하였다. 분석결과 Schuetteite (Hg<sub>3</sub>O<sub>2</sub>(SO<sub>4</sub>))라는 물질의 광물이 생성되었다. 본 광물은 결정체를 이루어 약 pH 2~3의 조건에서 일부 용출될 뿐 물리화학적으로 안정한 형태로 조사되었다. 그러므로 본 연구결과 국내 산업시설로부터 배출된 비산재를 대상으로 안정화 처리기술을 적용할 경우 염화수은 (HgCl<sub>2</sub>) 등 용해도가 높은 수은화학종을 지구상에 존재하는 광물로 전환시켜주는 Secondary Mineral 효과를 검증할 수 있었다. 단, 저농도 수은 오염 폐기물만을 대상으로 적용 가능한 기술이며 고농도의 경우 열적처리 등 수은을 제거할 수 있는 기술적용이 필요하다. 사사: 이 연구는 국립환경과학원의 “수은 유출저감을 위한 BAT 및 BEP 관리방안” 연구 용역 및 한국에너지기술평가원의 “에너지인력양성사업”에서 지원받았으며, 이에 감사드립니다.