Can the Palatine Tonsil be a Source of Mesenchymal Stem Cells with Immunomodulatory Property?

이윤세,박희영,임윤성,이진춘,왕수건,정진섭,이병주 대한이비인후과학회 부산,울산,경남 지부회 2012 임상이비인후과 Vol.23 No.1

Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cell types and are isolated from various other human adult and fetal tissues. Our study is to isolate palatine tonsil-derived MSCs (T-MSCs) and evaluate their differentiation potential and immunomodulatory effects, compared with bone marrow-derived MSCs (BM-MSCs). Methods:T-MSCs were isolated from human palatine tonsil. The expression of surface markers of T-MSCs was assessed by flow cytometric analysis. Differentiation potential of T-MSCs was analyzed histochemically and by the expression of lineage-related marker genes. Immunomodulatory effects were evaluated by mixed lymphocyte reactions (MLR) and mitogen proliferation assays with phytohemagglutinin (PHA). Results:T-MSCs were isolated from palatine tonsil and displayed a similar morphology to BM-MSCs. T-MSCs were negative for CD 31, CD45, CD117, HLA-DR and positive for CD44, CD73, CD90, and CD105 in flow cytometric analysis. The expression of surface phenotypes and differentiation potential of T-MSCs exhibited the similar finding as BM-MSCs. T-MSCs showed the significant inhibition of the proliferation of T cells stimulated by allergenic T cells in MLR study and by PHA stimuli in mitogen proliferation assay and also significant decrease of the secretion of TNF-α and IFN-γ (p<0.05). The HLA-G5 secretion by T-MSCs showed the significant increase in MLR and mitogen proliferation assays (p<0.05). T-MSCs do satisfy the phenotypical and functional definition of MSCs. T-MSCs also displayed immunomodulatory effects that were associated with inhibiting T-cell proliferation, decreasing soluble factors, and increasing HLA-G5 secretion in response to various stimuli. Conclusions:We propose that T-MSCs provide a good alternative for allogeneic MSCs in therapeutic applications.

두경부 편평상피세포암에 대한 최근 약제 개발과 연구 방향

이윤세 대한이비인후과학회 2019 대한이비인후과학회지 두경부외과학 Vol.62 No.9

After FDA approval of cetuximab at 2006, receptor tyrosine kinase, including an epidermalgrowth factor receptor, blocking agents have been evaluated for head and neck squamous cellcarcinoma (HNSCC). Agents targeting PI3K/Akt/mTOR, IL-6/JAK/STAT3, vascular endothelialgrowth factor receptor, and cyclin D-CDK-4/6-INK4/Rb pathway have developed. Most ofthem have failed to demonstrate better treatment outcome in recurrent and/or metastatic (R/M)HNSCC than conventional chemotherapy. Since a pivotal role of PD-1/PD-L1 pathway in immunologicaltumor microenvironment was revealed, the immune checkpoint inhibitors, includingpembrolizumab and nivolumab, have opened new paradigm of cancer treatment modality andpropagates other immune-based therapies for R/M HNSCC. Various types of combination trialsconsisting of immunotherapy with other class of immunotherapy, targeted agents, radiationtherapy, or conventional chemotherapy have been under investigation to improve treatment outcome. Biomarker studies to find an optimal candidate for the newly developed agents are accompanied. These clinical trials lead to tailored approach based on immunotherapy with precisionmedicine is expected to lead to promising results.

부식성 손상, 경부척추 손상 후 발생하는 연하장애

이윤세 대한연하장애학회 2018 대한연하장애학회지 Vol.8 No.1

Caustic injury and spinal cord injury may induce pharyngeal and esophageal dysfunction, which frequently elicit dysphagia. Among the causes of dysphagia, these types of injury are related to anatomical and functional deterioration of the peripheral muscles and nerves. Various types of chemical materials cause upper aerodigestive tract burns, which induces stricture and dysphagia by scar formation. Endoscopic evaluation within 48 hours helps to predict the occurrence of stricture and dysphagia. The extent of injury or other additional complications should be assessed by plain X-ray and comupterized tomography (CT). The prevention of stricture, perforation, and dysphagia is a mainstay of treatment to reduce morbidity, and serial dilation with esophagogram and flap reconstruction should be considered in failure cases. Cervical spine injury itself causes mucosal tears or neuromuscular dysfunction, which can occur even during corrective surgery. Perforation should be evaluated by laryngoscopy, swallowing studies, plain X-ray, and CT. Both mechanical and functional obstruction that occur during the healing process can be managed by rehabilitative or surgical approaches. Dysphagia caused by chemical burns or cervical spine injury can provoke secondary complications such as aspiration pneumonia and malnutrition. Thus, adequate evaluation and management of anatomical and functional changes are required to prevent dysphagia and further complications, as well as to increase the quality of life of patients.

인후두역류증의 진단

이윤세,이진춘 대한이비인후과학회 부산,울산,경남 지부회 2009 임상이비인후과 Vol.20 No.1

조기 후두암 환자에서 보전적 후두수술 후 음성 변화

이윤세,박정제,최승호,김상윤,남순열,Lee, Yoon-Se,Park, Jung-Je,Choi, Seung-Ho,Kim, Sang-Yoon,Nam, Soon-Yuhl 대한후두음성언어의학회 2004 대한후두음성언어의학회지 Vol.15 No.2

Objectives : The total laryngectomy for laryngeal cancer has made patients be afraid of voice loss. Early staged glottic or supraglottic cancer can be treated with conservative laryngeal surgery which preserve voice, though which was not normal voice comparing before. Voice analysis is used to evaluates objectively the quality of the voice in pre- and postoperation, 4 different types of conservative laryngeal surgery : laser cordectomy, supracticoid partial laryngectomy, vertical partial laryngectomy, and supralottic laryngectomy. Materials and Methods : The patients who received conservative laryngeal surgery(laser cordectomy : 23 cases, vertical partial laryngecotmy : 9cases, supracriocoid partial laryngectomy : 6cases, supraglottic laryngectomy : 8cases) from 1995 to 2001 in the Asan medical center. Fundamental frequency(F0), shimmer, jitter, noise to harmony ratio(NHR), maximum comfortable phonation time and subglottic pressure were used as parameters for voice analysis. Results : The patients who received laser cordectomy(shimmer : 5.26${\pm}$1.12%, jitter : 3.33${\pm}$0.42%, NHR : 0.47${\pm}$0.02, MPT : 9.32${\pm}$3.59sec) and supraglottic laryngectomy(shimmer : 4.39${\pm}$1.03%, jitter : 1.49${\pm}$0.14%, NHR : 0.51${\pm}$0.06, MPT : 8.9${\pm}$0.59sec) showed better results than other two procedures, but differed from normal value. Especially the patients who received supracricoid partial laryngectomy(shimmer : 9.23${\pm}$1.56%, jitter : 5.81${\pm}$1.23%, NHR : 5.89${\pm}$1.13, MPT : 6.3${\pm}$1.18sec, MFR : 632${\pm}$89ml/sec) had poorer quality of voice but presented fast functional recovery time, and the subjective symptom was improved as time goes by slowly. Conclusion : The appropriate conservative laryngeal surgery for each cancers and stage can preserve the acceptable voice for patients. Supracricoid partial laryngectomy for T1b glottic cancer can be used for acceptable voice despite its poor voice analysis.

Carcinoma Ex Pleomorphic Adenoma(CXPA)의 임상적 예후 인자

이윤세,현상민,임길채,최승호,김상윤,남순열 대한이비인후과학회 부산,울산,경남 지부회 2009 임상이비인후과 Vol.20 No.1

Carcinoma ex plemorphic adenoma (CXPA) originated from the salivary glands is rare but aggressive. It is known to be a high-grade carcinoma, frequently showing poor prognosis due to disease- related death and metastasis. Materials and Methods:We reviewed medical charts of patients who were diagnosed to have CXPA and received treatment from January, 1990 to July, 2008 at Asan medical center. Results: Thirty one patients (male:female=20:11) underwent surgery and/or adjuvant radiation therapy. The parotid gland was the most common site (74%). The tumors in the parotid were resected in the method of simple excision (4%), superficial parotidectomy (26%) or total parotidectomy with or without neck dissection (70%). Five year overall survival rate was 84.07%. According to site or treatment method in parotid gland originated tumor, there was no significant difference in survival rate and local control rate. Postoperative pathologic status of margin was the only factor influencing prognosis (p=0.04). Conclusion:Proper excision is a mainstay in the treatment of CXPA and preoperative evaluation of tumor extent is important. (J Clinical Otolaryngol 2009; 20:49-54)

하유리피판의 재건 실패 환자에서 보존적 치료로 치유한 2증례

이윤세,최성원,임윤성,왕수건 대한이비인후과학회 부산,울산,경남 지부회 2010 임상이비인후과 Vol.21 No.2

Since the introduction of microvascular surgery, free flap reconstruction has become an integral part of the management of head and neck cancer. Although microvascular skills and instruments have improved, failure of free flap reconstruction is inevitable and frequently leads to devastating results. Most of failed cases are caused by vascular thrombosis that is usually treated by either medical therapy or salvage reconstruction. When patient cannot run the risk of additional surgery, salvage reconstruction is not feasible. Conservative treatment after failure of free flap reconstruction would be another option in carefully selected cases. Conservative care contains medical management, watchful observation for fistula formation and frequent dressing. We reports two cases of patients who were treated with conservative management after failure of free flap reconstruction and reviewed literatures. (J Clinical Otolaryngol 2010;21:283-287)

소아 후두기관 협착의 치료

이윤세 대한후두음성언어의학회 2022 대한후두음성언어의학회지 Vol.33 No.3

Pediatric laryngotracheal stenosis occurs by either congenital or acquired causes and usually indicates subglottic stenosis. The main goals of treatment are decannulation, preserving phonation, and normal swallowing function. Various types and degrees of stenosis and combined anomalies would be the main barriers to reaching successful treatment results unless comprehensive understanding of stenosis. Multidisciplinary team approaches encompassing initial assessment, treatment, and postoperative care, are also necessary to achieve the best treatment outcome. Therapeutic approaches are divided into conservative, endoscopic, and open surgical approaches at length, which are not exclusive to each other. Here, an adequate selection of each therapeutic option and postoperative management will be introduced to achieve decannulation without leaving phonatory or swallowing complications.

울산지역 대기 중 아황산가스의 시공간적 오염특성(2005-2009)

이윤세 ( Yun Se Lee ),최성득 ( Sung Deuk Choi ),권혜옥 ( Hye Ok Kwon ),김철수 ( Chul Su Kim ),손희식 ( Hee Sik Son ),예진 ( Jin Ye ) 한국환경분석학회 2011 환경분석과 독성보건 Vol.14 No.4

We investigated the temporal and spatial distribution of SO2 measured at 14 air pollution monitoring sites in Ulsan, Korea. The levels of SO2 in Ulsan have continuously decreased since the 1990s, and a recent (2005-2009) annual average concentration of 8 ppb is lower than the national guideline of 20 ppb. However, Ulsan has higher levels of SO2 than any other mega-city in Korea. The average level of SO2 at industrial sites (13.7ppb) is 2.4 times higher than those at residential and commercial sites. According to the measurement data, meteorological condition, and former air dispersion modeling results, it was confirmed that SO2 emitted from the industrial complex was transported to the residential area by sea-land breeze and southeastern seasonal winds. For the improved air quality in Ulsan, the current environmental policy permitting the use of high-sulfur oil (<4%) should be carefully evaluated. The emission of greenhouse gases and hazardous air pollutants (HAPs) as well as SO2 from plants using the high-sulfur oil should be monitored. Furthermore, separated environmental policies for the industrial and residential areas are required for more effective pollution management.

소독제에 의한 콕시디움오오시스트의 포자형성 억제효과

이윤세,지차호 충북대학교 동물의학연구소 2005 Journal of Biomedical and Translational Research Vol.6 No.2

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